Simultaneous determination of luteolin and luteoloside in dandelions using HPLC

Abstract

The content of the two flavonoids, luteolin and luteoloside, in dandelions was determined by high-performance liquid chromatography. A Nucleosil®100-5C18 column was used for the determination of luteolin and luteoloside concentrations. The mobile phase was a mixture of acetonitrile and water containing 0.1% formic acid (99.9:0.1, v/v). The gradient elution program was 10% to 23% acetonitrile within 60 min at a flow rate of 1.0 mL·min−1. The ultraviolet spectra were recorded at 350 nm for the identification of the two flavonoids. The contents of luteolin and luteoloside were highest in T. officinale flowers (5.817 ± 0.030 mg·g−1) and in T. coreanum leaves (6.205 ± 0.087 mg·g−1), respectively. The total content of luteolin and luteoloside in T. coreanum was about 1.3–2 times higher than that found in T. ohwianum and T. officinale.

This is a preview of subscription content, log in to check access.

Literature Cited

  1. Akashi, T., T. Furuno, T. Takahashi, and S.I. Ayabe. 1994. Biosynthesis of triterpenoids in cultured cells, and regenerated and wild plant organs of Taraxacum officinale. Phytochemistry 36:303–308.

    Article  CAS  Google Scholar 

  2. Faber, K. 1958. Dandelion-Taraxacum officinale Weber. Pharmazie 13:423–436.

    PubMed  CAS  Google Scholar 

  3. Hänsel, R., M. Kartarahardja, J.T. Huang, and F. Bohlmann. 1980. Sesquiterpenlacton-ß-D-glucopyranoside sowie ein neues Eudesmanolid aus Taraxacum officinale. Phytochemistry 19:857–861.

    Article  Google Scholar 

  4. Hartwig, U.A., C.A. Maxwell, C.M. Joseph, and D.A. Phillips. 1990. Chrysoeriol and luteolin released from Alfalfa seeds induce nod genes in Rhizobium meliloti. Plant Physiol. 92:116–122.

    PubMed  Article  CAS  Google Scholar 

  5. Ho, C., E.J. Choi, G.S. Yoo, K.M. Kim, and S.Y. Ryu. 1998. Desacety lmatricarin, an anti-allergic component from Taraxacum platycarpum. Planta Med. 64:577–578.

    PubMed  Article  CAS  Google Scholar 

  6. Hook, I., A. McGee, and M. Henman. 1993. Evaluation of dandelion for diuretic activity and variation in potassium content. Int. J. Pharmacol. 31:29–34.

    Article  Google Scholar 

  7. Hu, C. and D.D. Kitts. 2004. Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW264.7 cells. Mol. Cellul. Biochem. 265:107–113.

    Article  CAS  Google Scholar 

  8. Hu, C. and D.D. Kitts. 2005. Dandelion (Taraxacum officinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro. Phytomedicine 12:588–597.

    PubMed  Article  CAS  Google Scholar 

  9. Jung, M.J., S.S. Kang, H.A. Jung, G.A. Kim, and J.S. Choi. 2004. Isolation of flavonoids and cerebroside from the stem bark of Albizzia julibrissin. Arch. Pharm. Res. 27:593–599.

    PubMed  Article  CAS  Google Scholar 

  10. Khan, I.A. and E.A. Abourashed. 2010. Leung’s Encyclopedia of Common Natural Ingredients. Used in Food, Drugs and Cosmetics, John Wiley & Sons, New Jersey, U.S.A.

    Google Scholar 

  11. Kisiel, W. and B. Barszcz. 2000. Further sesquiterpenoids and phenolics from Taraxacum officinale. Fitoterapia 71:269–273.

    PubMed  Article  CAS  Google Scholar 

  12. Kisiel, W. and K. Michalska. 2006. Matricarin-type guaianolides from Taraxacum bessarabicum and their chemotaxonomic significance. Biochem. Syst. Ecol. 34:356–359.

    Article  CAS  Google Scholar 

  13. Lee, H.H. and S.Y. Lee. 2008. Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai and T. officinale WEB. extracts. Korean J. Medicinal Crop Sci. 16:79–85.

    Google Scholar 

  14. Markham, K.R., V.M. Chari., and T.J. Mabry. 1982. Carbon-13 NMR spectroscopy of flavonoids. In “The flavonoids: Advances in research”, Chapman & Hall, London, England.

    Google Scholar 

  15. Michalska, K. and W. Kisiel. 2003. Sesquiterpene lactones from Taraxacum obovatum. Planta Med. 69:181–183.

    PubMed  Article  CAS  Google Scholar 

  16. Shin, K.H., S.S. Kang, E.A. Seo, and S.W. Shin. 1995. Isolation of aldose reductase inhibitors from the flowers of Chrysanthemum boreale. Arch. Pharm. Res. 18:65–68.

    Article  CAS  Google Scholar 

  17. Sweeney, B., M. Vora, C. Ulbricht, and E. Basch. 2005. Evidence-based systematic review of dandelion (Taraxacum officinale) by natural standard research collaboration. J. Herb. Pharmacother. 5:79–93.

    PubMed  Google Scholar 

  18. Takasaki, M., T. Konoshima, H. Tokuda, K. Masuda, Y. Arai, K. Shiojima, and H. Ageta. 1999. Anti-carcinogenic activity of Taraxacum plant. I. Biol. Pharm. Bull. 22:602–605.

    Article  CAS  Google Scholar 

  19. Tita, B., U. Bello, P. Faccendini, R. Bartolini, and P. Bolle. 1993. Taraxacum officinale W.: Pharmacological effect of ethanol extract. Pharmacol. Res. 27:23–24.

    Google Scholar 

  20. Williams, C.A., F. Goldstone, and J. Greenham. 1996. Flavonoids, cinnamic acids and coumarins from the different tissues and medicinal preparations of Taraxacum officinale. Phytochemistry 42:121–127.

    PubMed  Article  CAS  Google Scholar 

  21. Wolbis, M., M. Królikowska, and P. Bednarek. 1993. Polyphenolic compounds in Taraxacum officinale. Acta Pol. Pharm.-Drug Res. 50:153–158.

    CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Sanghyun Lee.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lee, S., Han, S., Kim, H.M. et al. Simultaneous determination of luteolin and luteoloside in dandelions using HPLC. Hortic. Environ. Biotechnol. 52, 536 (2011). https://doi.org/10.1007/s13580-011-0214-5

Download citation

Additional key words

  • isolation
  • phytochemical
  • quantitative analysis
  • structure determination