Skip to main content

Antioxidant and nitric oxide release inhibition activities of methanolic extract from Clerodendrum cyrtophyllum Turcz

Abstract

Antioxidant and anti-inflammatory activities of methanolic extract of Clerodendrum cyrtophyllum Turcz (ECT) were investigated in the present study. ECT showed high phenolic content (223 μg·GAE−1 equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC50 = 52.74 μg·mL−1) and reduced power ability. Furthermore, ECT exhibited significant protective ability in H2O2/Fe3+ /ascorbic acid-induced DNA and protein damage. Moreover, ECT inhibited NO production by 47.15% at 0.8 mg·mL−1 in lipopolysaccharides-stimulated RAW264.7 macrophages. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis showed that the expression of iNOS and COX-2 in RAW264.7 cells was down-regulated after treatment with increasing concentration of ECT. In conclusion, ECT has shown potent antioxidant and anti-inflammatory activities could be considered as a source of health promoting antioxidant and complementary medicinal supplement.

This is a preview of subscription content, access via your institution.

Literautre Cited

  • Aremu, A.O., A.R. Ndhlala, O.A. Fawole, M.E. Light, J.F. Finnie, and J. Van Staden. 2010. In vitro pharmacological evaluation and phenolic content of ten South African medicinal plants used as anthelmintics. S. Afr. J. Bot. 76:558–566.

    Article  Google Scholar 

  • Armstrong, C., W. Leong., and G.J. Lees. 2001. Comparative effects of metal chelating agents on the neuronal cytotoxicity induced by copper (Cu+2), iron (Fe+3) and zinc in the hippocampus. Brain Res. 892:51–62.

    PubMed  Article  CAS  Google Scholar 

  • Berker, K.I., K. Güçlü, İ Tor, and R. Apak. 2007. Comparative evaluation of Fe (III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents. Talanta 72:1157–1165.

    PubMed  Article  CAS  Google Scholar 

  • Barzilai, A., S. Biton, and Y. Shiloh. 2008. The role of the DNA damage response in neuronal development, organization and maintenance. DNA Repair 7:1010–1027.

    PubMed  Article  CAS  Google Scholar 

  • Chun, S.S., D.A. Vattem, Y.T. Lin., and K. Shetty. 2005. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem. 40:809–816.

    Article  CAS  Google Scholar 

  • Cai, Y., Q. Luo, M. Sun, and K. Corke. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74:2157–2184.

    PubMed  Article  CAS  Google Scholar 

  • Dinis, T.C., V.M. Madeira, and L.M. Almeida. 1994. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315:161–169.

    PubMed  Article  CAS  Google Scholar 

  • Duh, P.D., Y.Y. Tu, and G.C. Yen. 1999. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). Lebensm. Wiss. Technol. 32:269–277.

    Article  CAS  Google Scholar 

  • Gouthamchandra, K., R. Mahmood, and H. Manjunatha. 2010. Free radical scavenging, antioxidant enzymes and wound healing activities of leaves extracts from Clerodendrum infortunatum L. Environ. Toxicol. Pharmacol. 30:11–18.

    Article  CAS  Google Scholar 

  • Hu, W., W. Shen, and M.H. Wang. 2009. Free radical scavenging activity and protective ability of methanolic extract from Duchesnea indica against protein oxidation and DNA damage. J. Food Sci. Nutr. 14:277–282.

    Article  CAS  Google Scholar 

  • Je, J.Y., P.J. Park, E.K. Kim, and C.B. Ahn. 2009. Antioxidant and angiotensin I converting enzyme inhibitory activity of Bambusae caulis in Liquamen. Food Chem. 113:932–935.

    Article  CAS  Google Scholar 

  • Li, Y., Q.C. Zhao, T. Guo, and L.J. Wu. 2008. Chemical constituents of Clerodendrum cyrtophyllum Turcz. Chin. J. Med. Chem. 85: 371–373.

    Google Scholar 

  • Li, C., H.J. Son, C. Huang, S.K. Lee, J. Lohakare, and M.H. Wang. 2010. Comparison of Crataegus Pinnatifida Bunge var. typica Schneider and C. pinnatifida Bunge fruits for antioxidant, anti-glucosidase, and anti-inflammatory activities. Food Sci. Biotechnol. 19:769–775.

    Article  CAS  Google Scholar 

  • Luthria, D.L. 2008. Influence of experimental conditions on the extraction of phenolic compounds from parsley (Petroselinum crispum) flakes using a pressurized liquid extractor. Food Chem. 107:745–752.

    Article  CAS  Google Scholar 

  • Mandel, S., T. Amit, O. Bar-Am, and M.B.H. Youdim. 2007. Iron dysregulation in Alzheimer’s disease: Multimodal brain permeable iron chelating drugs, possessing neuroprotective-neurorescue and amyloid precursor protein-processing regulatory activities as therapeutic agents. Prog. Neurobiol. 82:348–360.

    PubMed  Article  CAS  Google Scholar 

  • Martell, A.E., R.J. Motekaitis, Y. Sun, R. Ma, M.J. Welch, and T. Pajeau. 1999. New chelating agents suitable for the treatment of iron overload. Inorganica Chim. Acta. 291:238–246.

    Article  CAS  Google Scholar 

  • Mayo, J.C., D.X. Tan, R.M. Sainz, M. Natarajan, S. Lopez-Burillo, and R.J. Reiter. 2003. Protection against oxidative protein damage induced by metal-catalyzed reaction or alkylperoxyl radicals: comparative effects of melatonin and other antioxidants. Biophys. Acta. 1620:139–150.

    CAS  Google Scholar 

  • Nandita, S. and P.S. Rajini. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem. 85:611–616.

    Article  Google Scholar 

  • Nagai, H., H. Kumamoto, M. Fukuda, and T. Takahashi. 2003. Inducible nitric oxide syntheses and apoptosis-related factors in the synovial tissues of temporomandibular joints with internal derangement and osteoarthritis. J. Oral Maxillofac. Surg. 61:801–807.

    PubMed  Article  Google Scholar 

  • Osadebe, P.O. and F.B.C. Okoye. 2003. Anti-inflammatory effects of crude methanolic extract and fractions of Alchornea cordifolia leaves. J. Ethnopharmacol. 89:19–24.

    PubMed  Article  CAS  Google Scholar 

  • Song, Y.G., B. Liu, L.F. Wang, M.H. Li, and Y. Liu. 2006. Damage to the oxygen-evolving complex by superoxide anion, hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II. Photosyn. Res. 90:67–78.

    PubMed  Article  CAS  Google Scholar 

  • Scandalios, J.G. 2005. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Braz. J. Med. Biol. Res. 38:995–1014.

    PubMed  Article  CAS  Google Scholar 

  • Shu, X.S., G.H. Gao, and H.L. Yang. 2006. Anti-inflammatory and anti-nociceptive activities o Smilax china L. aqueous extract. J Ethnopharmacol. 103:327–332.

    PubMed  Article  Google Scholar 

  • SchÖneich, C. 1999. Reactive oxygen species and biological aging a mechanistic approach. Exp. Gerontol. 34:19–34.

    PubMed  Article  Google Scholar 

  • Shetty, K. 2004. Role of proline-linked pentose phosphate pathway in biosynthesis of plant phenolics for functional food and environmental applications: a review. Process Biochem. 39:789–803.

    Article  CAS  Google Scholar 

  • Soares, J.R., T.C.P. Dins, A.P. Cunha, and L.M. Ameida. 1997. Antioxidant activity of some extracts of Thymus zygis. Free Radic. Res. 26:469–478.

    PubMed  Article  CAS  Google Scholar 

  • Wanasundara, U.N. and F. Shahidi. 1998. Antioxidant and pro-oxidant activity of green tea extracts in marine oils. Food Chem. 63:335–342.

    Article  CAS  Google Scholar 

  • Wang, Y.C., Y.C. Chuang, and H.W. Hsu. 2008. The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan. Food Chem. 106:277–284.

    Article  CAS  Google Scholar 

  • Xie, Q.W., H.J. Cho, J. Calaycay, R.A. Mumford, K.M. Swiderek, T.D. Lee, A. Ding, T. Troso, and C. Nathan. 1992. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256:225–228.

    PubMed  Article  CAS  Google Scholar 

  • Yoo, H.J., H.J. Kang, H.J. Jung, K. Kim, C.J. Lim, and E.H. Park. 2008. Anti-inflammatory, anti-angigeonic and anti-nociceptive activities of Saururus chinensis extract. J. Ethnopharmacol. 120:282–286.

    PubMed  Article  Google Scholar 

  • Zhang, H.Q., C.J. Huang, and Y.S. Wang. 1993. Pharmacological studies of Clerodendrum cyrtophyllum Turcz. J. Guilin. Med. Colg. 1:11–13. (in Chinese)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Myeong-Hyeon Wang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Liu, H., Wang, L. & Wang, MH. Antioxidant and nitric oxide release inhibition activities of methanolic extract from Clerodendrum cyrtophyllum Turcz. Hortic. Environ. Biotechnol. 52, 309–314 (2011). https://doi.org/10.1007/s13580-011-0204-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-011-0204-7

Additional key words

  • 1,1-diphenyl-2-picrylhydrazyl (DPPH)
  • anti-inflammatory
  • DNA damage
  • protein damage
  • semi-quantitative RT-PCR