Skip to main content

Antioxidant and nitric oxide release inhibition activities of methanolic extract from Clerodendrum cyrtophyllum Turcz

Horticulture, Environment, and Biotechnology volume 52, pages 309–314 (2011)Cite this article

Abstract

Antioxidant and anti-inflammatory activities of methanolic extract of Clerodendrum cyrtophyllum Turcz (ECT) were investigated in the present study. ECT showed high phenolic content (223 μg·GAE−1 equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC50 = 52.74 μg·mL−1) and reduced power ability. Furthermore, ECT exhibited significant protective ability in H2O2/Fe3+ /ascorbic acid-induced DNA and protein damage. Moreover, ECT inhibited NO production by 47.15% at 0.8 mg·mL−1 in lipopolysaccharides-stimulated RAW264.7 macrophages. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis showed that the expression of iNOS and COX-2 in RAW264.7 cells was down-regulated after treatment with increasing concentration of ECT. In conclusion, ECT has shown potent antioxidant and anti-inflammatory activities could be considered as a source of health promoting antioxidant and complementary medicinal supplement.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

Literautre Cited

  • Aremu, A.O., A.R. Ndhlala, O.A. Fawole, M.E. Light, J.F. Finnie, and J. Van Staden. 2010. In vitro pharmacological evaluation and phenolic content of ten South African medicinal plants used as anthelmintics. S. Afr. J. Bot. 76:558–566.

    Article  Google Scholar 

  • Armstrong, C., W. Leong., and G.J. Lees. 2001. Comparative effects of metal chelating agents on the neuronal cytotoxicity induced by copper (Cu+2), iron (Fe+3) and zinc in the hippocampus. Brain Res. 892:51–62.

    PubMed  Article  CAS  Google Scholar 

  • Berker, K.I., K. Güçlü, İ Tor, and R. Apak. 2007. Comparative evaluation of Fe (III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents. Talanta 72:1157–1165.

    PubMed  Article  CAS  Google Scholar 

  • Barzilai, A., S. Biton, and Y. Shiloh. 2008. The role of the DNA damage response in neuronal development, organization and maintenance. DNA Repair 7:1010–1027.

    PubMed  Article  CAS  Google Scholar 

  • Chun, S.S., D.A. Vattem, Y.T. Lin., and K. Shetty. 2005. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem. 40:809–816.

    Article  CAS  Google Scholar 

  • Cai, Y., Q. Luo, M. Sun, and K. Corke. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74:2157–2184.

    PubMed  Article  CAS  Google Scholar 

  • Dinis, T.C., V.M. Madeira, and L.M. Almeida. 1994. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315:161–169.

    PubMed  Article  CAS  Google Scholar 

  • Duh, P.D., Y.Y. Tu, and G.C. Yen. 1999. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). Lebensm. Wiss. Technol. 32:269–277.

    Article  CAS  Google Scholar 

  • Gouthamchandra, K., R. Mahmood, and H. Manjunatha. 2010. Free radical scavenging, antioxidant enzymes and wound healing activities of leaves extracts from Clerodendrum infortunatum L. Environ. Toxicol. Pharmacol. 30:11–18.

    Article  CAS  Google Scholar 

  • Hu, W., W. Shen, and M.H. Wang. 2009. Free radical scavenging activity and protective ability of methanolic extract from Duchesnea indica against protein oxidation and DNA damage. J. Food Sci. Nutr. 14:277–282.

    Article  CAS  Google Scholar 

  • Je, J.Y., P.J. Park, E.K. Kim, and C.B. Ahn. 2009. Antioxidant and angiotensin I converting enzyme inhibitory activity of Bambusae caulis in Liquamen. Food Chem. 113:932–935.

    Article  CAS  Google Scholar 

  • Li, Y., Q.C. Zhao, T. Guo, and L.J. Wu. 2008. Chemical constituents of Clerodendrum cyrtophyllum Turcz. Chin. J. Med. Chem. 85: 371–373.

    Google Scholar 

  • Li, C., H.J. Son, C. Huang, S.K. Lee, J. Lohakare, and M.H. Wang. 2010. Comparison of Crataegus Pinnatifida Bunge var. typica Schneider and C. pinnatifida Bunge fruits for antioxidant, anti-glucosidase, and anti-inflammatory activities. Food Sci. Biotechnol. 19:769–775.

    Article  CAS  Google Scholar 

  • Luthria, D.L. 2008. Influence of experimental conditions on the extraction of phenolic compounds from parsley (Petroselinum crispum) flakes using a pressurized liquid extractor. Food Chem. 107:745–752.

    Article  CAS  Google Scholar 

  • Mandel, S., T. Amit, O. Bar-Am, and M.B.H. Youdim. 2007. Iron dysregulation in Alzheimer’s disease: Multimodal brain permeable iron chelating drugs, possessing neuroprotective-neurorescue and amyloid precursor protein-processing regulatory activities as therapeutic agents. Prog. Neurobiol. 82:348–360.

    PubMed  Article  CAS  Google Scholar 

  • Martell, A.E., R.J. Motekaitis, Y. Sun, R. Ma, M.J. Welch, and T. Pajeau. 1999. New chelating agents suitable for the treatment of iron overload. Inorganica Chim. Acta. 291:238–246.

    Article  CAS  Google Scholar 

  • Mayo, J.C., D.X. Tan, R.M. Sainz, M. Natarajan, S. Lopez-Burillo, and R.J. Reiter. 2003. Protection against oxidative protein damage induced by metal-catalyzed reaction or alkylperoxyl radicals: comparative effects of melatonin and other antioxidants. Biophys. Acta. 1620:139–150.

    CAS  Google Scholar 

  • Nandita, S. and P.S. Rajini. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem. 85:611–616.

    Article  Google Scholar 

  • Nagai, H., H. Kumamoto, M. Fukuda, and T. Takahashi. 2003. Inducible nitric oxide syntheses and apoptosis-related factors in the synovial tissues of temporomandibular joints with internal derangement and osteoarthritis. J. Oral Maxillofac. Surg. 61:801–807.

    PubMed  Article  Google Scholar 

  • Osadebe, P.O. and F.B.C. Okoye. 2003. Anti-inflammatory effects of crude methanolic extract and fractions of Alchornea cordifolia leaves. J. Ethnopharmacol. 89:19–24.

    PubMed  Article  CAS  Google Scholar 

  • Song, Y.G., B. Liu, L.F. Wang, M.H. Li, and Y. Liu. 2006. Damage to the oxygen-evolving complex by superoxide anion, hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II. Photosyn. Res. 90:67–78.

    PubMed  Article  CAS  Google Scholar 

  • Scandalios, J.G. 2005. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Braz. J. Med. Biol. Res. 38:995–1014.

    PubMed  Article  CAS  Google Scholar 

  • Shu, X.S., G.H. Gao, and H.L. Yang. 2006. Anti-inflammatory and anti-nociceptive activities o Smilax china L. aqueous extract. J Ethnopharmacol. 103:327–332.

    PubMed  Article  Google Scholar 

  • SchÖneich, C. 1999. Reactive oxygen species and biological aging a mechanistic approach. Exp. Gerontol. 34:19–34.

    PubMed  Article  Google Scholar 

  • Shetty, K. 2004. Role of proline-linked pentose phosphate pathway in biosynthesis of plant phenolics for functional food and environmental applications: a review. Process Biochem. 39:789–803.

    Article  CAS  Google Scholar 

  • Soares, J.R., T.C.P. Dins, A.P. Cunha, and L.M. Ameida. 1997. Antioxidant activity of some extracts of Thymus zygis. Free Radic. Res. 26:469–478.

    PubMed  Article  CAS  Google Scholar 

  • Wanasundara, U.N. and F. Shahidi. 1998. Antioxidant and pro-oxidant activity of green tea extracts in marine oils. Food Chem. 63:335–342.

    Article  CAS  Google Scholar 

  • Wang, Y.C., Y.C. Chuang, and H.W. Hsu. 2008. The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan. Food Chem. 106:277–284.

    Article  CAS  Google Scholar 

  • Xie, Q.W., H.J. Cho, J. Calaycay, R.A. Mumford, K.M. Swiderek, T.D. Lee, A. Ding, T. Troso, and C. Nathan. 1992. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256:225–228.

    PubMed  Article  CAS  Google Scholar 

  • Yoo, H.J., H.J. Kang, H.J. Jung, K. Kim, C.J. Lim, and E.H. Park. 2008. Anti-inflammatory, anti-angigeonic and anti-nociceptive activities of Saururus chinensis extract. J. Ethnopharmacol. 120:282–286.

    PubMed  Article  Google Scholar 

  • Zhang, H.Q., C.J. Huang, and Y.S. Wang. 1993. Pharmacological studies of Clerodendrum cyrtophyllum Turcz. J. Guilin. Med. Colg. 1:11–13. (in Chinese)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon, 200-701, Korea

    Huan Liu, Lan Wang & Myeong-Hyeon Wang

Authors
  1. Huan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Myeong-Hyeon Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Myeong-Hyeon Wang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Liu, H., Wang, L. & Wang, MH. Antioxidant and nitric oxide release inhibition activities of methanolic extract from Clerodendrum cyrtophyllum Turcz. Hortic. Environ. Biotechnol. 52, 309–314 (2011). https://doi.org/10.1007/s13580-011-0204-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-011-0204-7

Additional key words

  • 1,1-diphenyl-2-picrylhydrazyl (DPPH)
  • anti-inflammatory
  • DNA damage
  • protein damage
  • semi-quantitative RT-PCR