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Efficient in vitro plant regeneration from hybrid rhizomes of Cymbidium sinense seeds

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Abstract

An oriental orchid that has leaves striped with different colors is considered to be one of the valuable hybrid cultivars. To propagate this hybrid cultivar, C. sinense Willd Makino (Kumwhasan, Bosei) and C. sinense Willd Makino (Seobo) were crossed. The crossed seeds were cultivated in vitro to induce rhizome development since rhizome-mediated propagation can shorten the long-term (by at least 6 years) propagation time and overcome variations induced by seedling propagation. Knudson and/or Hyponex medium with or without natural products were used for the rhizome and shoot induction. The optimum concentration of activated charcoal (AC) was 0.025% for the best shoot induction rate in line No. 115-1 where 1 mg · L−1 of NAA and 0.5 mg · L−1 of BA were added to the Hyponex medium and in line No. 115-2 where only natural products were added to the Knudson plus Hyponex medium. However, 0.2% of AC was shown to be optimal for line No. 118, which was grown on Hyponex medium without plant growth regulators.

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Literature Cited

  • Arditti, J. 1977. Clonal propagation of orchids by means of tissue culture — a manual. In: J. Arditti (ed.). Orchid Biology, Vol. I, p. 203–293. Cornell University Press, New York.

    Google Scholar 

  • Bechtel, H., P. Cribb, and E. Launert. 1992. The manual of cultivated orchid species, 3rd ed. MIT Press, Cambridge, Massachusetts.

    Google Scholar 

  • Carlberg, I., K. Glimelius, and T. Eriksson. 1983. Improved culture ability of patato protoplasts by use of activated charcoal. Plant Cell Rep. 2:223–225.

    Article  Google Scholar 

  • Champagnat, M.M., G. Morel, and G. Gambade. 1968. Particularites morphologiques et pouvoir de regeneration de Cymbidium virescence cultive in vitro. Soc. Bot. Fr. Mem. 115:236–249.

    Google Scholar 

  • Chang, C. and W.C. Chang. 2000. Micropropagation of Cymbidium ensifolium var. misericors through callus-derived rhizomes. In Vitro Cell. Dev. Biol. Plant 36:517–520.

    Article  CAS  Google Scholar 

  • Chen, Y., X. Liu, and Y. Liu. 2005. In vitro plant regeneration from the immature seeds of Cymbidium faberi. Plant Cell Tissue Organ Cult. 81:247–251.

    Article  Google Scholar 

  • Choi, S.O., J.D. Chung, and J.H. Lee. 1996. Effect of culture media on rhizome formation and its subsequent growth from shoot-tip culture of temperate Cymbidium species. Kor. J. Plant Tissue Cult. 23:167–172.

    Google Scholar 

  • Chung, J.D., C.C. Ki, and S.S. Kim. 1983. Asymbiotic germination and plant culture of Aerides japonicum. Selection of suitable medium and culture condition for germination and effect of hyponex, peptone, and several nutrient supplements on growth of seedling. J. Kor. Soc. Hort. Sci. 25:305–312.

    Google Scholar 

  • Chung, J.D., C.K. Chun, and S.O. Choi. 1985a. Asymiotic germination of Cymbidium ensifolium. II. Effects of several supplements to the medium, pH values and light and/or dark culture periods on growth of rhizome and organogenesis from rhizome. J. Kor. Soc. Hort. Sci. 26:186–192.

    CAS  Google Scholar 

  • Chung, J.D., C.K. Chun, S.S. Kim, and J.S. Lee. 1985b. Factors affecting growth of rhizome and organogenesis of Korean Cymbidium karan. J. Kor. Soc. Hort. Sci. 26:281–288.

    Google Scholar 

  • Fridborg, G., M. Pedersen, L.E. Landstrom, and T. Eriksson. 1978. The effect of activated charcoal on tissue cultures: adsorption of metabolites inhibiting morphogenesis. Physiol. Plant 43:104–106.

    Article  CAS  Google Scholar 

  • Hasegawa, A., H. Ohashi, and M. Go. 1985. Effect of BA, rhizome length, mechanical treatment and liquid shaking culture on the shoot formation from rhizome in Cymbidium faberi Rolfe. Acta Hort. 166:25–40.

    Google Scholar 

  • Horner, M., J.A. McComb, A.J. McComb, and H.E. Street. Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J. Exp. Bot. 28:1366–1372.

  • Kano, K. 1971. Seed germination of oriental Cymbidium and their shoot tip culture. Pro. 6th World Orchid Conference. p. 133–142.

  • Kauth, P.J., T.R. Johnson, S.L. Stewart, and M.E. Kane. 2008. A classroom exercise in hand pollination and in vitro asymbiotic orchid seed germination. Plant Cell Tissue Organ Cult. 93:223–230.

    Article  Google Scholar 

  • Koebner, R.M.D. 1995. Predigestion of DNA template improves the level of polymorphism of random amplified polymorphic DNAs in wheat. Genetic Analysis: Biomolecular Engineering 12:63–67.

    Article  CAS  Google Scholar 

  • Knudson, L. 1922. Nonsymbiotic germination of orchid seeds. Bot. Gaz. 73:1–25.

    Article  Google Scholar 

  • Liu, M.S.C. 1993. Plant regeneration in cell suspension culture of sugarcane as affected by activated charcoal, medium composition and tissue culture. Taiwan Sugar. 18–25. May–June.

  • Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15:743–747.

    Article  Google Scholar 

  • Nasrallah, M.E., J.T. Barber, and D.H. Wallce. 1969. Self-incompatibility proteins in plants: Detection, genetics and possible mode of action. Heridity 24:23–27.

    Google Scholar 

  • Nishio, T. and K. Hinata. 1977. Analysis of s-specific protein in stigma of Brassica oleracea L. by isoelectric focusing. Heredity 38:391–396.

    Article  Google Scholar 

  • Okasaki, K. and K. Hinata. 1984. Analysis of S-allele and S-glycoproteins in F1hybrid varieties of Japanese radish (Raphanus stivus L.) Japan. J. Breed. 34:237–245.

    Google Scholar 

  • Owen, H., D. Wengerd, and A.R. Miller. 1991. Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method. Plant Cell Rep. 10:583–386.

    Article  CAS  Google Scholar 

  • Roberts, I.N., A.D. Stead, D.J. Ochendon, and J.G. Dickinson. 1979. A glycoprotein associated with the acquisition of the self-incompatibility system by maturing stigmas of Brassica oleracea. Planta 146:179–183.

    Article  CAS  Google Scholar 

  • Sedgley, M. 1974. Assessment of serological techniques for S-allele identification in Brassica oleracea. Euphytica 23:543–551.

    Article  Google Scholar 

  • Shimasaki, K. and S. Uemoto. 1991. Rhizome induction and plantlet regeneration of Cymbidium goeringii from flower bud cultures in vitro. Plant Cell Tissue Organ Cult. 25:49–52.

    Article  CAS  Google Scholar 

  • Teixeria, J.B., M.R. Sondahl, and E.G. Kirby. 1994. Somatic embryogenesis from immature inflorescences of oil palm. Plant Cell Rep. 13:247–250.

    Google Scholar 

  • Theander, O. and D.A. Nelson. 1988. Aqueous, high temperature transformation of carbohydrates relative to utilization of biomass. Adv. Carbhydr. Chem. Biochem. 46:73–326.

    Article  Google Scholar 

  • Thomas, T.D. 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advances 26:618–631.

    Article  PubMed  CAS  Google Scholar 

  • Weatherhead, M.A., J. Burdon, and G.G. Henshaw. 1978. Some effects of activated charcoal as an additive to plant tissue culture media. Z. Pflanzenphysiol 89:141–147.

    CAS  Google Scholar 

  • Weatherhead, M.A., J. Burdon, and G.G. Henshaw. 1979. Effects of activated charcoal as an additive plant tissue culture media. Z. Pflanzenphysiol 94:399–405.

    CAS  Google Scholar 

  • Wu, Y. 1992. Introduction to Chinese native orchids. p. 48–154. In: Y. Wu (ed.). Chinese Orchids. Forestry Publication House of China, Beijing.

    Google Scholar 

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Correspondence to Byung-Hoon Min.

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Lee, O.R., Yang, DC., Chung, HJ. et al. Efficient in vitro plant regeneration from hybrid rhizomes of Cymbidium sinense seeds. Hortic. Environ. Biotechnol. 52, 303–308 (2011). https://doi.org/10.1007/s13580-011-0187-4

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  • DOI: https://doi.org/10.1007/s13580-011-0187-4

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