Abstract
Pathogenic variants of the KCNH1 gene can cause dominant-inherited Temple–Baraitser/Zimmermann–Laband syndrome with severe mental retardation, seizure, gingival hyperplasia and nail hypoplasia. This study established an induced pluripotent stem cell (iPSC) line using urinary cells from a girl with KCNH1 recurrent/hotspot pathogenic variant c.1070G > A (p.R357Q). The cell identity, pluripotency, karyotypic integrity, absence of reprogramming virus and mycoplasma contamination, and differential potential to three germ layers of the iPSC line, named as ZJUCHi003, were characterized and confirmed. Furthermore, ZJUCHi003-derived neurons manifested slower action potential repolarization process and wider action potential half-width than the normal neurons. This cell line will be useful for investigating the pathogenic mechanisms of KCNH1 variants-associated symptoms, as well as for evaluating novel therapeutic approaches.
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This study was supported by the Fundamental Research Funds for the Central Universities of China (226–2022-00035).
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Chen, D., Su, J., Huang, X. et al. Establishment and characterization of ZJUCHi003: an induced pluripotent stem cell line from a patient with Temple–Baraitser/Zimmermann–Laband syndrome carrying KCNH1 c.1070G > A (p.R357Q) variant. Human Cell 37, 832–839 (2024). https://doi.org/10.1007/s13577-024-01031-8
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DOI: https://doi.org/10.1007/s13577-024-01031-8