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Long non-coding RNA LBX2-AS1 predicts poor survival of colon cancer patients and promotes its progression via regulating miR-627-5p/RAC1/PI3K/AKT pathway

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Abstract

Colon cancer is one of the most prevalent malignant tumors across the world. Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) take part in colon cancer development. Our study intends to explore the expression characteristics of LBX2-AS1, a novel lncRNA, in colon cancer and its underlying mechanisms. The results illustrated that LBX2-AS1 level was substantially increased in colon cancer tissues and was obviously correlated with the tumor volume and early distant metastasis of patients. Besides, overexpression of LBX2-AS1 remarkably boosted growth, proliferation, and metastasis and restrained apoptosis in colon cancer cells, whereas LBX2-AS1 knockdown produced the opposite effect. On the other hand, miR-627-5p, down-regulated in colon cancer tissues, was negatively associated with LBX2-AS1 expression. Functional experiments showed that miR-627-5p suppressed colon cancer growth. Mechanistically, LBX2-AS1, as an endogenous competitive RNA, targeted miR-627-5p and restrained its expression, while miR-627-5p targeted and negatively regulated the RAC1/PI3K/AKT axis. Collectively, this study has revealed that LBX2-AS1 is a poor prognostic factor of colon cancer and can regulate colon cancer progression by regulating the miR-627-5p/RAC1/PI3K/AKT pathway.

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Availability of data and material

The data sets used and analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by Huazhong University of Science and Technology “Double Top” Construction Project of International Cooperation (grant 540–5001540013 to Feng JP) and Wuhan Municipal Health Commission (WX19D65 to Jing Fang).

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Authors and Affiliations

  1. Department of Oncology, Wuhan Fourth Hospital, PuAi Hospital of Tongji Medical College, Huazhong University of Science and Technology, No.76 Jiefang Road, Qiaokou District, Wuhan, 430034, Hubei, People’s Republic of China

    Jing Fang, Junyuan Yang, Hui Chen, Wen Sun, Lingyun Xiang & Jueping Feng

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  1. Jing Fang
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  2. Junyuan Yang
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  3. Hui Chen
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  4. Wen Sun
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  5. Lingyun Xiang
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  6. Jueping Feng
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Contributions

Conceived and designed the experiments: JF, JF. Performed the experiments: JF, Junyuan Yang. Statistical analysis: HC, WS. Formal analysis: LX. Funding acquisition: Jing Fang, JF. Wrote the paper: JF. All authors read and approved the final manuscript.

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Correspondence to Jueping Feng.

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The authors declare that they have no competing interests.

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Our study was approved by the Ethics Review Board of Wuhan Fourth Hospital.

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Fang, J., Yang, J., Chen, H. et al. Long non-coding RNA LBX2-AS1 predicts poor survival of colon cancer patients and promotes its progression via regulating miR-627-5p/RAC1/PI3K/AKT pathway. Human Cell 35, 1521–1534 (2022). https://doi.org/10.1007/s13577-022-00745-x

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