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Enzyme-digested Colla Corii Asini (E’jiao) accelerates wound healing and prevents ultraviolet A-induced collagen synthesis decline and wrinkle formation in three-dimensional skin equivalents

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A Correction to this article was published on 19 October 2020

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Abstract

Cutaneous wound healing delay, collagen synthesis decline and wrinkle formation are the common features of skin aging. The aim of this study is to investigate repressive effects of Colla Corii Asini (CCA) (a traditional Chinese medicine which has been used for anti-aging) on hydrogen peroxide (300 µM, 2 h) and ultraviolet A (UVA) (3.2 mJ/cm2)-induced skin aging in vitro. To simulate the in vivo condition of CCA, CCA was digested by gastrointestinal enzymes and added to human gingival fibroblasts (HGF) and three dimensional (3D) skin equivalents at different concentrations. Cell viability assay showed that the enzyme-digested CCA (CCAD) exhibited significant preventive effects on hydrogen peroxide- and UVA-induced cell death. The in vitro scratch assay showed that CCAD was able to prevent hydrogen peroxide-induced wound healing delay in HGF cell sheets. Immunostaining and imaging analysis showed that CCAD could suppress UVA-reduced expression of type IV collagen and elastin in both HGF cells and the 3D skin equivalents. Using a tissue stretching system, wrinkles were formed on UVA-irradiated 3D skin equivalents. Without CCAD-treatment, the wrinkles on the skin were deep, whereas CCAD markedly reduced the depth of wrinkles. In conclusion, CCAD could protect skin cells from oxidative stress and UVA-induced harmful effects, accelerate wound healing, promote synthesis of collagen and elastin, and reduce wrinkles formation. CCAD might be developed as an anti-skin aging reagent in the cosmetic industry.

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Change history

  • 19 October 2020

    In the original version of the publication, the UVA dose and irradiation time were miscalculated.

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Acknowledgements

The present study was supported in part by a Grant-in-Aid for AntiAging Scientific Research #1517 by Japanese Center for AntiAging MedSciences, Hiroshima, Japan to LX and TaiShan Industrial Experts Program of China No. TSCY20170233 (to FL). The authors also appreciate Nathaniel Green’s proofreading.

Author information

Author notes

  1. Li Xiao and Feng Liao contributed equally to the present article.

Authors and Affiliations

  1. Department of Pharmacology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-0071, Japan

    Li Xiao

  2. National Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Liao Cheng, Shandong Province, China

    Feng Liao & Yumei Fan

  3. Faculty of Life Sciences, Prefectural University of Hiroshima, Hiroshima, Japan

    Nobuhiko Miwa

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  1. Li Xiao
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  2. Feng Liao
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  4. Nobuhiko Miwa
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by LX and FL. Some experiments were performed by YF. The first draft of the manuscript was written by LX. Nobuhiko Miwa revised the manuscript and commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Authors Feng Liao and Yumei Fan are employees of Dong-E-E-Jiao Co. Ltd.

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Correspondence to Li Xiao.

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Li Xiao and Nobuhiko Miwa have reported no potential conflict of interest relevant to this article.

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Xiao, L., Liao, F., Fan, Y. et al. Enzyme-digested Colla Corii Asini (E’jiao) accelerates wound healing and prevents ultraviolet A-induced collagen synthesis decline and wrinkle formation in three-dimensional skin equivalents. Human Cell 33, 1056–1067 (2020). https://doi.org/10.1007/s13577-020-00405-y

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