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MiR-96 promotes apoptosis of nucleus pulpous cells by targeting FRS2

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Abstract

This study aimed to investigate the molecular mechanism by which microRNA (miR)-96 regulates the progression of intervertebral disc degeneration (IDD). The expression of miR-96 in normal intervertebral discs and in IDD was detected by performing reverse transcription-quantitative PCR. CCK8 assay was applied to examine the proliferation of nucleus pulpous (NP) cells and flow cytometry was used to evaluate the cell apoptosis and cell cycle profile. In addition, the immunofluorescence analysis was employed to detect cell proliferation. The expressions of proteins were assessed by western blot analysis. TargetScan and miRDB were used to predict the target genes of miR-96. The results indicated that miR-96 expression was upregulated in IDD compared with normal intervertebral discs. Overexpression of miR-96 could significantly inhibit the proliferation of NP cells via inducing apoptosis and G1 arrest. In addition, fibroblast growth factor receptor substrate 2 (FRS2) was identified as the target of miR-96 and overexpression of FRS2 could revere the effect of miR-96 mimics in NP cells. Therefore, these findings demonstrated that miR-96 plays a critical role during the progression of IDD and miR-96 may serve as a target for the treatment of IDD.

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  • 08 September 2020

    In the original publication of the

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Funding

This study was funded by National Natural Science Foundation of China (81802190, 81772412), Shandong Medical and Health Technology Development Plan Project (2017WS033) and Youth Fund Project of the Affiliated Hospital of Qingdao University (2017).

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Authors and Affiliations

  1. Department of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China

    Xiaoxia Yang, Hengping Liu, Qingfeng Zhang, Kan Liu, Dong Yu, Yi Zhang & Zongting Shi

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  1. Xiaoxia Yang
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  2. Hengping Liu
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  3. Qingfeng Zhang
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  4. Kan Liu
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  5. Dong Yu
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Correspondence to Xiaoxia Yang.

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These authors declared no competing interests.

Ethical approval

All of the experiments were approved by the Ethics Committees of the Affiliated Hospital of Qingdao University (No. 2015006688). The informed consent forms were signed by patients or their families.

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Yang, X., Liu, H., Zhang, Q. et al. MiR-96 promotes apoptosis of nucleus pulpous cells by targeting FRS2. Human Cell 33, 1017–1025 (2020). https://doi.org/10.1007/s13577-020-00389-9

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  • DOI: https://doi.org/10.1007/s13577-020-00389-9

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