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MiR-645 regulates the proliferation and apoptosis of diffuse large B-cell lymphoma by targeting DACH1

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Abstract

Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of malignant non-Hodgkin lymphoma cases. An increasing body of evidence has indicated the critical roles of microRNAs (miRNAs) in regulating the progression of DLBCL. In this study, we found that miR-645 was up-regulated in DLBCL tissues and cell lines. Down-regulation of miR-645 significantly inhibited the proliferation, cell cycle progression and promoted the apoptosis of DLBCL cells. Experimental study identified Dachshund family transcription factor 1 (DACH1) as a target of miR-645. MiR-645 bound the 3′-untranslated region of DACH1 and reduced the expression of DACH1 in DLBCL cells. Decreased expression of DACH1 was inversely correlated with that of miR-645 in DLBCL tissues. The promoting effect of miR-645 on the proliferation of DLBCL cells was attenuated with the overexpression of DACH1. These results demonstrated the novel mechanism of miR-645 in DLBCL, which indicated miR-645 as a potential target for the diagnosis and prognostics of DLBCL.

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Acknowledgements

This work was supported by the Cangzhou Science and Technology Support Technology Project (No. 141302108).

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Authors and Affiliations

  1. The Second Hematology Department, Cangzhou Central Hospital, No. 16 Xinhua West Street, Cangzhou, 061001, Hebei, China

    Ruihuan Wang, Jie Shen, Qing Wang, Minjuan Zhang & Chunyan Liu

  2. The CDC of Xinhua District, Cangzhou, 061000, Hebei, China

    Na Su

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  1. Ruihuan Wang
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  2. Jie Shen
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  3. Na Su
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  4. Qing Wang
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  6. Chunyan Liu
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Correspondence to Ruihuan Wang.

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Wang, R., Shen, J., Su, N. et al. MiR-645 regulates the proliferation and apoptosis of diffuse large B-cell lymphoma by targeting DACH1. Human Cell 33, 1091–1098 (2020). https://doi.org/10.1007/s13577-020-00321-1

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  • DOI: https://doi.org/10.1007/s13577-020-00321-1

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