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LncRNA SNHG8 promotes proliferation and invasion of gastric cancer cells by targeting the miR-491/PDGFRA axis

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Abstract

This study aimed to investigate the effects of long non-coding small nucleolar RNA host gene 8 (SNHG8) on the proliferation and invasion of gastric cancer (GC). The GC tissues and adjacent normal tissues from 30 patients were collected. Human GC cell lines, including AGS, SGC-7901, MKN-1, and BGC-803 and normal human gastric epithelial cell line GES-1 were purchased and cultured. The Cell Counting Kit-8 and Western blots were used to access cell proliferation, and platelet-derived growth factor receptor α (PDGFRA) expression, respectively. qRT-PCR was used to evaluate the expression of SNHG8 and miRNA-491. A transwell assay was applied to evaluate cell invasion. This study illustrated that SNHG8 expression was upregulated in GC tissues, as well as in cell lines. Moreover, knockdown of SNHG8 inhibited GC cell proliferation and invasion. Significantly, we determined that miRNA-491 was not only downregulated in stomach cancer but also inhibited GC cell progression induced by SNHG8. Further investigation demonstrated that SNHG8 promoted the proliferation and invasion of GC cells by targeting the miR-491/PDGFRA axis. LncRNA SNHG8 promoted the proliferation and invasion of GC cells by targeting the miR-491/PDGFRA axis, which might provide new insight for potential therapeutic strategies for GC in the future.

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Author notes

  1. Peigen Zhang and Suting Li contributed equally to this work.

Authors and Affiliations

  1. Department of Emergency Services, Zengcheng District People’s Hospital of Guangzhou, Zengjiang Street, Zengcheng District, Guangzhou, 511300, People’s Republic of China

    Peigen Zhang & Huanyao Zhang

  2. Department of Nursing, Zengcheng District People’s Hospital of Guangzhou, Guangzhou, China

    Suting Li

  3. Department of Outpatient Internal Medicine, Zengcheng District People’s Hospital of Guangzhou, Guangzhou, China

    Zhijun Chen

  4. Department of Surgery, Zengcheng District People’s Hospital of Guangzhou, Guangzhou, China

    Yan Lu

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  1. Peigen Zhang
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  2. Suting Li
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  3. Zhijun Chen
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  4. Yan Lu
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  5. Huanyao Zhang
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Contributions

PZ and SL performed the experiments. ZC and YL analyzed the data. HZ designed the study and drafted the manuscript.

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Correspondence to Huanyao Zhang.

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Zhang, P., Li, S., Chen, Z. et al. LncRNA SNHG8 promotes proliferation and invasion of gastric cancer cells by targeting the miR-491/PDGFRA axis. Human Cell 33, 123–130 (2020). https://doi.org/10.1007/s13577-019-00290-0

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  • DOI: https://doi.org/10.1007/s13577-019-00290-0

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