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ADRB2 suppresses IL-13-induced allergic rhinitis inflammatory cytokine regulated by miR-15a-5p

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Abstract

Allergic rhinitis (AR) is a common hypersensitive disease that troubles patients a lot. Nasal epithelial cells (NECs), as the outmost protection of inhalation, play an important role in AR allergic response. Adrenoceptor beta 2 (ADRB2) is an important gene in inflammatory response, which has become the hot spot for AR development and treatment in recent years. MiR-15a-5p has been proved to be involved in AR immune response as the upstream regulator of ADRB2. Human primary NECs were isolated and stimulated by IL-13. qRT-PCR assay was used to detect the RNA level of target genes. ELISA and Western blotting were applied to detect target protein levels. Luciferase reporter assay and biotin pull-down assay were performed to test molecules interaction. ADRB2 was highly expressed in nasal mucosa of AR patients and was positively correlated with IL-13 stimulation, and knockdown of ADRB2 inhibited IL-13-induced expression of GM-CSF, eotaxin, and MUC5AC in NECs. ADRB2 was directly targeted by miR-15a-5p, and miR-15a-5p inhibited IL-13-induced expression of GM-CSF, eotaxin, and MUC5AC in NECs. ADRB2 mediated the effect of miR-15a-5p on the regulation of nasal epithelial immune responses. ADRB2 is negatively regulated by miR-15a-5p, which inhibits IL-13-induced nasal epithelial inflammatory responses.

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Funding

The study was supported by the National Natural Science Foundation of China (81670907).

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Author notes

  1. Li Wang, Qiaoying Lv and Xicheng Song contributed equally to this work.

Authors and Affiliations

  1. Department of Otorhinolaryngology Head and Neck Surgery, Yuhuangding Hospital, Yantai, 264000, Shandong, China

    Li Wang, Qiaoying Lv, Xicheng Song & Junhong Zhang

  2. Department of Orthopaedic Surgery, Yuhuangding Hospital, Yantai, 264000, Shandong, China

    Kun Jiang

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  1. Li Wang
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  2. Qiaoying Lv
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  4. Kun Jiang
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Correspondence to Li Wang, Kun Jiang or Junhong Zhang.

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Wang, L., Lv, Q., Song, X. et al. ADRB2 suppresses IL-13-induced allergic rhinitis inflammatory cytokine regulated by miR-15a-5p. Human Cell 32, 306–315 (2019). https://doi.org/10.1007/s13577-019-00259-z

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  • DOI: https://doi.org/10.1007/s13577-019-00259-z

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