Human Cell

, Volume 31, Issue 2, pp 116–126 | Cite as

Flavone protects HBE cells from DNA double-strand breaks caused by PM2.5

  • Xing Ren
  • Yong Tang
  • Jiameng Sun
  • Jianbo Feng
  • Leilei Chen
  • Huixi Chen
  • Sijing Zeng
  • Changhui Chen
  • Xinqiu Li
  • Haixia Zhu
  • Zhaojun Zeng
Research Article


Ambient air particulate matter 2.5 (PM2.5) contains many harmful components that can enter the circulatory system and produce reactive oxygen species (ROS) in body. Oxidative stress and DNA damage induced by ROS may affect any cellular macromolecule and lead to DNA double-strand breaks (DSBs). Flavonoids, widely distributed in some herbs and berries, have been proved having anti-oxidative or anti-cancer efficacy. In this study, we investigated whether Flavone, a kind of flavonoids, can protect human bronchial epithelial cells (HBE) from DSBs caused by PM2.5 and how this function is probably implemented. We found that cells exposed to PM2.5 obviously induced viability inhibition, DNA damage and part of apoptosis. However, Flavone treatment prior to PM2.5 apparently improved cell viability, and mitigated the formation of 8-hydroxy-2-deoxyguanosine, the expression of DNA damage-relative protein and cell apoptosis. Our studies demonstrated that PM2.5 induced oxidative DSBs while Flavone ameliorated the DNA damage and increased cell viability probably through influencing DNA repair mechanism of cells.


PM2.5 Flavone Double-strand breaks DNA damage repair 



This project was supported by National Natural Science Foundation of China (30600753, 81172154) and 2016 Mittal Student Innovation Project of Central South University (12700, 160230001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13577_2017_193_MOESM1_ESM.jpg (196 kb)
Supplementary material 1 (JPEG 195 kb) Fig. S1 HBE cells were exposed to flavone only at the concentration of 100 μM for 4 h, and then cultured for 24 h. (A) Cell viability was measured using CCK-8. (B) and (C) The levels of γ-H2AX were detected Immunofluorescent and Western blot. (D) and (E) The apoptosis were detected by Flow cytometry
13577_2017_193_MOESM2_ESM.jpg (18 kb)
Supplementary material 2 (JPEG 18 kb) Fig. S2 HBE cells were exposed to flavone at the different concentration for 4 h prior to 128 μg/mL PM2.5 exposure for 24 h. Western blotting was used to detect the expression of DNA damage and repair proteins


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Xing Ren
    • 1
  • Yong Tang
    • 1
  • Jiameng Sun
    • 1
  • Jianbo Feng
    • 3
  • Leilei Chen
    • 4
  • Huixi Chen
    • 4
  • Sijing Zeng
    • 1
  • Changhui Chen
    • 1
  • Xinqiu Li
    • 4
  • Haixia Zhu
    • 5
  • Zhaojun Zeng
    • 1
    • 2
  1. 1.School of Life SciencesCentral South UniversityChangshaPeople’s Republic of China
  2. 2.State Key Laboratory of Medical GeneticsCentral South UniversityChangshaPeople’s Republic of China
  3. 3.Cancer Research Institute, School of Basic Medical ScienceCentral South UniversityChangshaPeople’s Republic of China
  4. 4.Xiangya School of MedicineCentral South UniversityChangshaPeople’s Republic of China
  5. 5.The Third Hospital of XiangyaCentral South UniversityChangshaPeople’s Republic of China

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