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Circadian variation of cell proliferation in HTR-8/SVneo cell line

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Abstract

Circadian clock controls several physiological processes such as cell proliferation. Extravillous trophoblast proliferation is a tightly regulated function playing a fundamental role in maternal vessel remodeling. We recently demonstrated that clock genes Per2 and Dec1 as well as the clock-controlled genes Dbp and Vegf are rhythmically expressed in human extravillous trophoblast-derived HTR-8/SVneo cells. Analyzing the time course of HTR-8/SVneo cell proliferation, a circadian variation in cell number was found. Moreover, we showed a rhythmic expression of mRNAs for Wee1 and stathmin, two genes involved in cell cycle progression. We suggest that circadian clockwork may orchestrate the functionality of the several factors involved in the control of human trophoblast functions that are fundamental for a successfully pregnancy outcome.

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Acknowledgments

This work was supported by research grants of the University of Ferrara and MIUR (C. Bertolucci and C. Biondi). L. Lunghi was supported by a fellowship from the Fondazione della Cassa di Risparmio di Ferrara.

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Authors and Affiliations

  1. Department of Biology and Evolution, University of Ferrara, Via L. Borsari, 46, 44100, Ferrara, Italy

    Laura Lunghi, Elena Frigato, Maria Enrica Ferretti, Carla Biondi & Cristiano Bertolucci

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  1. Laura Lunghi
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  2. Elena Frigato
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  3. Maria Enrica Ferretti
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  4. Carla Biondi
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  5. Cristiano Bertolucci
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Corresponding author

Correspondence to Laura Lunghi.

Additional information

L. Lunghi and E. Frigato contributed equally to this work.

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Lunghi, L., Frigato, E., Ferretti, M.E. et al. Circadian variation of cell proliferation in HTR-8/SVneo cell line. Human Cell 24, 161–164 (2011). https://doi.org/10.1007/s13577-011-0032-1

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  • DOI: https://doi.org/10.1007/s13577-011-0032-1

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