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Chemical or enzymatic deglycosylation and germination abrogates the inhibitory activity of Cyamopsis tetragonoloba trypsin inhibitor

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Abstract

A glycosylated heat stable trypsin chymotrypsin inhibitor was isolated from Cyamopsis tetragonoloba seeds. It is being reported for the first time that deglycosylation of the inhibitor chemically by Trifluoro methane sulfonic acid or enzymatically by salivary amylase or fungal diastase abrogates the inhibitory activity indicating that glycosylation was important for inhibitory activity of the protein. Treatment with salivary amylase decreases the inhibition in in vitro digestion experiment also. Germination of Cyamopsis tetragonoloba seeds results in reduction in inhibitory activity with concomitant increase in amylase activity. It is speculated that increase in amylase during germination decreases the protease inhibitory activity by deglycosylating the inhibitor.

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Abbreviations

CTTI:

Cyamopsis tetragonoloba trypsin inhibitor

TFMS:

Trifluoro methane sulfonic acid

BAPNA:

Nα-Benzyl-L-arginine 4-nitroanilide

SDS-PAGE:

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

DEAE:

Diethylaminoethyl

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Acknowledgements

Financial assistance from University Grant Commission INDIA as National OBC fellowship (F./2017−18/NFO−2017−18-OBC-MAD−48042) to P.P .and M.B. (NFO−2018−2019-OBC-MAD−77987) is gratefully acknowledged.

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Authors and Affiliations

  1. School of Life Sciences, Devi Ahilya University, Khandwa Road Campus, 452001, Indore, India

    Preeti Patidar, Mamta Bhayal & Krishnan Hajela

  2. Department of Biotechnology IPS Academy, Rajendra Nagar, Indore, India

    Sumati Hajela

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  1. Preeti Patidar
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  2. Mamta Bhayal
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  3. Sumati Hajela
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  4. Krishnan Hajela
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Correspondence to Krishnan Hajela.

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Patidar, P., Bhayal, M., Hajela, S. et al. Chemical or enzymatic deglycosylation and germination abrogates the inhibitory activity of Cyamopsis tetragonoloba trypsin inhibitor. J. Plant Biochem. Biotechnol. 31, 961–964 (2022). https://doi.org/10.1007/s13562-022-00777-2

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  • DOI: https://doi.org/10.1007/s13562-022-00777-2

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