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A fungal mycelium elicitor efficiently improved ginsenoside synthesis during adventitious root culture of Panax ginseng

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Abstract

To select an effective and economic elicitor for the production of ginsenosides by adventitious root culture of Panax ginseng C.A. Mey, this study used mycelia of the pathogenic fungi Alternaria panax as a biotic elicitor to investigate effects of elicitor concentrations and elicitation duration on ginsenoside accumulation; the elicitation effect of mycelia and its extract as well as methyl jasmonate was also compared. The concentrations of the mycelium elicitor and elicitation duration significantly affected ginsenoside accumulation, and the maximum ginsenoside contents were obtained when 30-day-old adventitious roots were treated with 200 mg/L mycelium elicitor for 8 days. In comparison experiment, ginsenoside accumulation of mycelium elicitor was similar to that of the mycelium extract elicitor and approximately 25 mg/g DW of ginsenoside content was obtained, which was significantly higher (p < 0.05) than that of methyl jasmonate. Consequently, the mycelium was considered to be the more suitable elicitor because it can effectively improve ginsenoside accumulation with easily and simply preparing. The finding of the present study suggested that the mycelia of Alternaria panax can be used as an economic elicitor during adventitious root culture of Panax ginseng for the commercial production of ginsenosides in the future.

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Data are available by e-mail on reasonable request.

Abbreviations

AR::

Adventitious root

DW:

Dry weight

FEex:

Mycelium extract elicitor

FEmy:

Mycelium elicitor

HPLC:

High-performance liquid chromatography

MeJA:

Methyl jasmonate

MS:

Murashige and Skong medium

SD:

Standard deviation

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Funding

This work was supported by the National Natural Science Foundation of China (81960685).

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Authors and Affiliations

  1. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, China

    X. L. An, Y. Yu, M. Z. Fan, X. H. Wu, X. F. Li, X. C. Piao & M. L. Lian

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  1. X. L. An
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  2. Y. Yu
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  3. M. Z. Fan
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  4. X. H. Wu
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  5. X. F. Li
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  6. X. C. Piao
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  7. M. L. Lian
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Contributions

Conceptualization and methodology: [XLA] and [YY]; Formal analysis and investigation: [MZF] and [XHW]; Validation: [XFL]; Data curation: [XCP]; Writing-review and editing: [MLL]. All authors have read and approved the final version of the manuscript.

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Correspondence to M. L. Lian.

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An, X.L., Yu, Y., Fan, M.Z. et al. A fungal mycelium elicitor efficiently improved ginsenoside synthesis during adventitious root culture of Panax ginseng. J. Plant Biochem. Biotechnol. 31, 657–664 (2022). https://doi.org/10.1007/s13562-021-00759-w

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