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Plant growth-promoting bacteria (PGPBs) and copper (II) oxide (CuO) nanoparticle ameliorates DNA damage and DNA Methylation in wheat (Triticum aestivum L.) exposed to NaCl stress

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Abstract

Wheat is the second important cereal crop worldwide due to nutritional composition and role in meeting daily energy needs. Salinity is an abiotic stress factor that restricts crop productivity through influencing plant growth and development in arid and semi-arid regions. Nanomaterials and plant growth promoting bacteria (PGPBs) can be used in many different areas of agriculture for different purposes. In this study, changes in cytosine methylation and DNA damage levels in wheat (Triticum aestivum L.) exposed to salt stress (250 mM NaCl) were determined and possible preventive effects of copper (II) oxide nanoparticles (0, 50 and 100 mg/L; CuO-Nps > 100 nm) and plant growth promoting bacteria (no bacteria, Bacillus subtilis, Lactobacillus casei, Bacillus pumilis; PGPBs) treatments were investigated. Changes in cytosine methylation were analyzed by Coupled Restriction Enzyme Digestion-iPBS (CRED-iPBS) and genotoxic influences and genomic stability was analyzed with the aid of inter-primer binding site (iPBS) primers. Application of 250 mM NaCl remarkably increased polymorphism rate of iPBS profile. Besides, relieve effect of PGPBs with CuO-NPs was observed against adverse effect of 250 mM NaCl stress. The genomic template stability values clearly increased with PGPBs with CuO-NPs treatments, particularly Lactobacillus casei with 100 mg/L of CuO-Nps. In addition, DNA hypo-methylation was observed in all treatments. As a conclusion, PGPBs with CuO-NPs treatments showed a strong anti-genotoxic effect against NaCl stress and they could be used as an alternative molecule to alleviate genetic impairment in wheat under NaCl stress.

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Abbreviations

PGPBs:

Plant growth-promoting bacteria

PCR:

Polymerase chain reaction

CuO:

Copper (II) oxide

NP:

Nanoparticle

CRED:

Coupled Restriction Enzyme Digestion

iPBS:

Inter-primer binding site

GTS:

Genomic template stability

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Authors and Affiliations

  1. Department of Field Crops, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey

    Arash Hosseinpour, Güller Özkan & Kamil Haliloglu

  2. Crop and Horticultural Science Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Ardabil, Moghan, Iran

    Arash Hosseinpour

  3. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, 25050, Erzurum, Turkey

    Emre Ilhan

  4. Health Services Vocational School, Erzincan Binali Yıldırım University, Erzincan, Turkey

    Halil İbrahim Öztürk

  5. Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey

    Kağan Tolga Cinisli

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  1. Arash Hosseinpour
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  2. Emre Ilhan
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  3. Güller Özkan
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  4. Halil İbrahim Öztürk
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  5. Kamil Haliloglu
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  6. Kağan Tolga Cinisli
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Contributions

AH conceived and designed the experiments and worked for writing and editing of the English of this paper. TC, EI, GO, HIO and KH performed the experiments, collected and analyzed the data. EI went through literature and helped in drafting the manuscript.

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Correspondence to Emre Ilhan.

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Hosseinpour, A., Ilhan, E., Özkan, G. et al. Plant growth-promoting bacteria (PGPBs) and copper (II) oxide (CuO) nanoparticle ameliorates DNA damage and DNA Methylation in wheat (Triticum aestivum L.) exposed to NaCl stress. J. Plant Biochem. Biotechnol. 31, 751–764 (2022). https://doi.org/10.1007/s13562-021-00713-w

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