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Flavonoid production and antioxidative activity in liquid-cultured hairy roots of Apocynum venetum

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Abstract

Apocynum venetum is a traditional and popular Chinese herb used for medicine and tea, and its major bioactive components is flavonoid. This study aimed to analyze the flavonoid production and antioxidative activity of the hairy roots under 250 mL shake flask and 3 L bubble bioreactor cultivation. Agrobacterium rhizogenes strain Ar.1193 was used to induce hairy roots at the wounding site of Apocynum venetum stems, and a 69.17% genetic transformation rate was noted. Hairy roots with the most vigorous growth were selected to analyze growth and flavonoid production over one complete growth cycle (30 d). The dry weight of the roots increased tenfold, flavonoid contents peaked at 25 d under shake flask and bioreactor cultivation, and the flavonoid content of roots in the bioreactor was 43.97% higher than that of roots in the shake flask. The flavonoid content and antioxidative activity of liquid-cultured hairy roots were significantly higher than those of field-planted roots, tissue-cultured plant roots, and callus. Moreover, a total of 117 flavonoids were detected in hairy roots. The results of the present study highlight hairy roots of Apocynum venetum could serve as an alternative to whole plants and may be used for the large-scale production of flavonoid.

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Abbreviations

WPM:

Woody plant medium

LB:

Luria–Bertani

AS:

Acetosyringone

Cef:

Cefotaxime sodium

PCR:

Polymerase chain reaction

FRAP:

Ferric reducing antioxidant power

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

QQQ:

Triple quadrupole

Q TRAP:

Quadrupole-linear ion trap mass spectrometer

MRM:

Multiple reaction monitoring

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Funding

This work was supported by the Foundation of State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences (G2018-02–07), Heilongjiang Natural Science Foundation of China (C2016005).

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Authors and Affiliations

  1. College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin, 150000, China

    Lixiang Zhang & Haiyan Wang

  2. Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin, 150040, China

    Zhong Yang Yu, Ya Guang Zhan & Gui Zhi Fan

  3. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China

    Li Jiang

Authors
  1. Lixiang Zhang
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  2. Zhong Yang Yu
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  3. Haiyan Wang
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  4. Li Jiang
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  5. Ya Guang Zhan
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  6. Gui Zhi Fan
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Contributions

G-ZF and Y-GZ conceived and designed the experiments. L-XZ and H-YW performed the research. LJ and Z-YY analyzed the data. G-ZF and L-XZ wrote the paper. All authors have read and approved the final manuscript.

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Correspondence to Gui Zhi Fan.

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The authors declared that they have no conflict of interest.

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Zhang, L., Yu, Z.Y., Wang, H. et al. Flavonoid production and antioxidative activity in liquid-cultured hairy roots of Apocynum venetum. J. Plant Biochem. Biotechnol. 31, 554–560 (2022). https://doi.org/10.1007/s13562-021-00707-8

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  • DOI: https://doi.org/10.1007/s13562-021-00707-8

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