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Sequestering miR165/166 enhances seed germination in Arabidopsis thaliana under normal condition and ABA treatment

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Abstract

Seed germination is a dynamic process involving imbibition, increased metabolic activity and protrusion of a tiny plantlet rupturing the seed coat. Many genes, phytohormones like ABA and GA have been implicated in germination of Arabidopsis thaliana seeds. Although many microRNAs (miRNAs) have been shown to be differentially expressed during seed germination process, their role remains mostly unaddressed. Here we address the role of developmentally important miR165/166 in the process of seed germination. We demonstrate that the seeds of transgenic A. thaliana having target mimic-miR165/166 (eTM-miR165/166), where miR165/166 is sponged, show better germination efficiency. The seeds of this line also maintain better germination even under ABA treatment, which is a negative regulator of seed germination. Thus, our results suggest that, sequestering miR165/166 activity enhances seed germination efficiency under normal and ABA-stress condition.

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Fig. 1

Abbreviations

ABA:

Abscisic acid

GA:

Gibberellic Acid

miRNA:

MicroRNA

eTM:

Endogenous Target Mimic

HD-ZIPIII:

Class III HOMEODOMAIN LEUCINE-ZIPPER

PHB:

PHABULOSA

PHV:

PHAVOLUTA

REV:

REVOLUTA

ATHB8/15:

ARABIDOPSIS THALIANA HOMEOBOX 8/15

STTM:

Short Tandem Target Mimic

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Acknowledgements

SSD and MM acknowledge financial and infrastructural support from NIPGR. SSD acknowledges DST-Women Scientist-A (WOS-A) fellowship from the Department of Science and Technology (DST), India (WOS-A/LS-1276/2014). We thank Dr A. K. Sarkar (NIPGR) for plant materials and support. PK, AKN, SSD thank Vidyasagar University for support.

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Authors and Affiliations

  1. Department of Botany & Forestry, Vidyasagar University, Midnapore, West Bengal, India

    Shabari Sarkar Das, Asis K. Nandi & Prakash Karmakar

  2. National Institute of Plant Genome and Research, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Shabari Sarkar Das & Manoj Majee

Authors
  1. Shabari Sarkar Das
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  2. Manoj Majee
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  3. Asis K. Nandi
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  4. Prakash Karmakar
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SSD performed the experiments and wrote the manuscript. MM, PK, and AKN contributed to the designing of experiments and improvement of manuscript.

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Correspondence to Shabari Sarkar Das.

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Sarkar Das, S., Majee, M., Nandi, A.K. et al. Sequestering miR165/166 enhances seed germination in Arabidopsis thaliana under normal condition and ABA treatment. J. Plant Biochem. Biotechnol. 29, 838–841 (2020). https://doi.org/10.1007/s13562-020-00607-3

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