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Overexpression of the sweetpotato peroxidase gene swpa4 enhances tolerance to methyl viologen-mediated oxidative stress and dehydration in Arabidopsis thaliana

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Abstract

We previously reported that transgenic Arabidopsis thaliana plants overexpressing the sweet potato peroxidase gene swpa4 under the control of the cauliflower mosaic virus (CaMV) 35 s promoter showed increased levels of reactive oxygen species (ROS) and nitric oxide (NO), and higher expression of ROS and NO related genes than control plants. Here, we investigated the effect of swpa4 overexpression on the abiotic and biotic stress tolerance levels of Arabidopsis plants. Methyl viologen (MV) treatment-induced oxidative stress caused visible damage to the seedlings and rosette leaves of all Arabidopsis plants, although the symptoms were more severe in control plants than in transgenic lines. Additionally, survival rates and ion leakage showed a slight decline in transgenic lines but a more severe decline in control plants after MV treatment. Transgenic plants also showed enhanced tolerance to drought stress. Dehydration treatment, followed by rehydration, resulted in a greater change in the relative water content and lipid peroxidation of control plants than in that of transgenic lines. However, transgenic plants did not show enhanced resistance to biotic stresses such as bacterial and fungal pathogens. These results indicate that transgenic Arabidopsis plants can efficiently regulate defense levels during oxidative stress via the overexpression of swpa4.

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Abbreviations

MV:

Methyl viologen

NO:

Nitric oxide

PR:

Pathogenesis-related

ROS:

Reactive oxygen species

RWC:

Relative water contents

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2018R1A1A1A05018446), and the Systems & Synthetic Agrobiotech Center (PJ01318401), the Biogreen 21 Project for the Next Generation, Rural Development Administration, Korea.

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Authors and Affiliations

  1. Department of Biology Education, College of Education, and IALS, Gyeongsang National University, Jinju, 660-701, Korea

    Yun-Hee Kim

  2. Department of Horticulture, Gyeongnam National University of Science & Technology, JinJu, Korea

    Jeum Kyu Hong

  3. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea

    Ho Soo Kim & Sang-Soo Kwak

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  1. Yun-Hee Kim
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  2. Jeum Kyu Hong
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  3. Ho Soo Kim
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  4. Sang-Soo Kwak
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Correspondence to Yun-Hee Kim or Sang-Soo Kwak.

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Kim, YH., Hong, J.K., Kim, H.S. et al. Overexpression of the sweetpotato peroxidase gene swpa4 enhances tolerance to methyl viologen-mediated oxidative stress and dehydration in Arabidopsis thaliana. J. Plant Biochem. Biotechnol. 30, 215–220 (2021). https://doi.org/10.1007/s13562-020-00588-3

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  • DOI: https://doi.org/10.1007/s13562-020-00588-3

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