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Green synthesis of silver nanoparticles using aqueous fruit and tuber extracts of Momordica cymbalaria

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Abstract

Plant extracts have been extensively used for the green synthesis of nanoparticles as they are rich in eco-friendly bioactive compounds possessing high metal-ion reducing potential. The present study is aimed at investigating the green synthesis of silver nanoparticles (AgNPs) by using aqueous fruit and tuber extracts of Momordica cymbalaria, also to test their antibacterial and cytotoxic potential. AgNP’s formation was noticed initially by the colour change in the reaction mixture i.e., green to red, which was confirmed further through a characteristic SPR peak at 446 nm and 432 nm respectively. The AgNP’s size, elemental property, phase purity and crystallite size was assessed through SEM, EDS and X-ray diffraction spectroscopy. The AgNPs exhibited prolonged stability infer to the layering of phenolic and protein moieties, identified by FTIR spectrophotometry. Tuber and fruit extracts prepared AgNPs were tested for anti-bacterial activity against pathogenic organisms and they have shown higher bactericidal effect against Staphylococcus aureus, and E. coli in comparison to Streptococcus pyogenes and Pseudomonas aeruginosa. In vitro cytotoxicity of tuber extract AgNPs against prostate (PC-3) and breast adenocarcinoma (MDA-MB 231) cells has given lower IC50 values (72.39 and 64.03 µg/ml, respectively, than the fruit extract prepared AgNPs (85.42 and 111.74 µg/ml, respectively). The study concludes that the fruit and tuber extracts of Momordica cymbalaria can be successfully used for the synthesis of AgNPs that exhibit effective anti-bacterial as well as cytotoxic potential.

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References

  • Alsalhi MS, Devanesan S, Alfuraydi AA, Vishnubalaji R, Munusamy MA, Murugan K, Nicoletti M, Benelli G (2016) Green synthesis of silver nanoparticles using Pimpinella anisum seeds: antimicrobial activity and cytotoxicity on human neonatal skin stromal cells and colon cancer cells. Int J Nanomed 11:4439–4449

    CAS  Google Scholar 

  • Altikatoglu M, Attar A, Erci F, Cristache CM, Isildak I (2017) Green synthesis of copper oxide nanoparticles using Ocimum basilicum extract and their antibacterial activity. Fresenius Environ Bull. 26(12A/2017):7832–7837

    CAS  Google Scholar 

  • Ashok Kumar S, Ravi S, Kathiravan V, Velmurugan S (2015) Synthesis of silver nanoparticles using A. Indicum leaf extract and their antibacterial activity. Spectrochim Acta Part A Mol Biomol Spectrosc 134:34–39

    CAS  Google Scholar 

  • Banerjee P, Satapathy M, Mukhopahayay A, Das P (2014) Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess 1:3

    Google Scholar 

  • Bonev B, Hooper J, Parisot J (2008) Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother 61:1295–1301

    CAS  PubMed  Google Scholar 

  • Bunghez IR, BarbintaPatrascu ME, Badea N, Doncea SM, Popescu A, Ion RM (2012) Antioxidant silver nanoparticles green synthesized using ornamental plants. Journal of optoelectronics and advanced materials. 14(11):1016–1022

    CAS  Google Scholar 

  • Byeth N, Houri-Haddad Y, Domb A, Khan W, Hazan R (2015) Alternative antimicrobial approach: nano-antimicrobial materials. Based Complement. Altern. Med, Evid. https://doi.org/10.1155/2015/246012

    Book  Google Scholar 

  • Colgan R, Williams M (2011) Diagnosis and treatment of acute uncomplicated cystitis. Am Fam Physician 84(7):771–776

    PubMed  Google Scholar 

  • Cullity BD, Stock SR (2001) Elements of X-ray diffraction, 3rd edn. Prentice-Hall, New York

    Google Scholar 

  • Dakal TC, Kumar A, Majumdar RS, Yadav V (2016) Mechanistic basis of antimicrobial actions of silver nanoparticles. Front Microbiol 7:1831

    PubMed  PubMed Central  Google Scholar 

  • David L, Moldovan B, Vulcu A, Olenic L, Perde-Schrepler M, Fischer-Fodor E, Florea A, Crisan M, Chiorean I, Clichici S, Filip GA (2014) Green synthesis, characterization and anti-inflammatory activity of silver nanoparticles using European black elderberry fruits extract. Colloids Surf B Biointerfaces. 122:767–777

    CAS  PubMed  Google Scholar 

  • Edremitlioğlu M, Kiliç D, Oter S, Kisa U, Korkmaz A, Coşkun O, Bedir O (2005) The effect of hyperbaric oxygen treatment on the renal functions in septic rats: relation to oxidative damage. Surg Today 35(8):653–661

    PubMed  Google Scholar 

  • Elliott C (2010) The effects of silver dressings on chronic and burns wound healing. Br J Nurs 19:S32–S36

    PubMed  Google Scholar 

  • Gupta M, Tomar RS, Kaushik S, Mishra RK, Sharma D (2018) Effective antimicrobial activity of green ZnO nano particles of Catharanthus roseus. Front Microbiol 9:2030

    PubMed  PubMed Central  Google Scholar 

  • Hemalatha S, Makeswari M (2017) Green synthesis, characterization and antibacterial studies of CuO nanoparticles from Eichhornia crassipes. Rasayan J. Chem. 10(3):838–843

    CAS  Google Scholar 

  • Henglein A (1998) Colloidal silver nanoparticles: photochemical preparation and interaction with O2, CCl4, and some metal ions. Chem Mater 10(1):444–450

    CAS  Google Scholar 

  • Huh AJ, Kwon YJ (2011) Nanoantibiotics: a new paradigm for treating infectious diseases using nanomaterials in the antibiotic resistant era. J Control Release 156:128–145

    CAS  PubMed  Google Scholar 

  • Jabir NR, Tabrez S, Ashraf GM, Shakil S, Damanhouri GA, Kamal MA (2012) Nanotechnology-based approaches in anticancer research. Int J Nanomed 7:4391–4408

    CAS  Google Scholar 

  • Jain S, Saxena S, Kumar A (2014) Epidemiology of prostate cancer in India. Meta Gene. 07:007

    Google Scholar 

  • Jiang H, Moon K, Zhang Z, Pothukuchi S, Wong CP (2006) Variable frequency microwave synthesis of silver nanoparticles. J Nanopart Res 8(1):117–124

    CAS  Google Scholar 

  • Johnsona AS, Obota IB, Ukponga US (2014) Green synthesis of silver nanoparticles using Artemisia annua and Sida acuta leaves extract and their antimicrobial, antioxidant and corrosion inhibition potentials. J Mater Environ Sci 5(3):899–906

    Google Scholar 

  • Kahraman O, Binzet R, Turunc E, Dogen A, Arslan H (2018) Synthesis, characterization, antimicrobial and electrochemical activities of zinc oxide nanoparticles obtained from Sarcopoterium spinosum (L.) Spach leaf extract. Mater Res Exp. https://doi.org/10.1088/2053-1591/aad953

    Article  Google Scholar 

  • Kelkawi AHA, AbbasiKajani A, Bordbar AK (2017) Green synthesis of silver nanoparticles using Mentha pulegium and investigation of their antibacterial, antifungal and anticancer activity. IET Nanobiotechnol 11(4):370–376

    PubMed  Google Scholar 

  • Koneri R, Balraman R, Saraswati CD (2006) Antiovulatory and abortifacient potential of the ethanolic extract of roots of Momordica cymbalaria Fenzl in rats. Indian J Pharmacol. 38:111

    Google Scholar 

  • Koneri RB, Samaddar S, Ramaiah CT (2014) Anti-diabetic activity of a triterpenoid saponin isolated from Momordica cymbalaria Fenzl. Indian J Exp Biol 52(1):46–52

    CAS  PubMed  Google Scholar 

  • Kovács D, Igaz N, Keskeny C, Bélteky P, Tóth T, Gáspár R, Madarász D, Rázga Z, Kónya Z, Boros IM, Kiricsi M (2016) Silver nanoparticles defeat p53-positive and p53-negative osteosarcoma cells by triggering mitochondrial stress and apoptosis. Sci Rep 6:27902

    PubMed  PubMed Central  Google Scholar 

  • Krishnaraj C, Jagan E, Rajasekar SS, Kalaichelvan P, Mohan N (2010) Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surfaces B Bio-interfaces. 76:50–56

    CAS  Google Scholar 

  • Krishnaraj C, Muthukumaran P, Ramachandran R, Balakumaran MD, Kalaichelvan PT (2014) Acalypha indica Linn: biogenic synthesis of silver and gold nanoparticles and their cytotoxic effects against MDA-MB-231, human breast cancer cells. Biotechnol Rep 4:42–49

    CAS  Google Scholar 

  • Kumar DA, Palanichamy V, Roopan SM (2014) Green synthesis of silver nanoparticles using Alternanthera dentata leaf extract at room temperature and their antimicrobial activity. Spectrochim Acta A Mol Biomol Spectrosc. 127:168–171

    CAS  PubMed  Google Scholar 

  • Luo L-B, Yu S-H, Qian H-S, Zhou T (2005) Large-scale fabrication of flexible silver/cross-linked poly(vinyl alcohol) coaxial nanocables by a facile solution approach. J Am Chem Soc 127(9):2822–2823

    CAS  PubMed  Google Scholar 

  • Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, Kalinina NO (2014) “Green” nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae. 6(1):35–44

    CAS  PubMed  PubMed Central  Google Scholar 

  • Mani A, Lakshmi GV (2012) Bio-mimetic synthesis of silver nanoparticles and evaluation of its free radical scavenging activity. Int J Biol Pharm Res 3(4):631–633

    Google Scholar 

  • Mathers CD, Loncar D (2006) Projections of global mortality and burden of disease from 2002 to 2030. PLoS Med. 3(11):e442

    PubMed  PubMed Central  Google Scholar 

  • Mohan CS, Sasikala K, Anand T, Vengaiah PC, Krishnaraj S (2014) Green synthesis, antimicrobial and antioxidant effects of silver nanoparticles using Canthium coromandelicum leaves extract. Res J Microbiol 9(3):142–150

    Google Scholar 

  • Mohanta YK, Panda SK, Jayabalan R, Sharma N, Bastia AK, Mohanta TK (2017) Antimicrobial, antioxidant and cytotoxic activity of silver nanoparticles synthesized by leaf extract of Erythrina suberosa (Roxb.). Front Mol Biosci 4:14

    PubMed  PubMed Central  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    CAS  PubMed  Google Scholar 

  • Nabikha K, Kathiersan A, Raj M, Alikunhi N (2009) Synthesis of antimicrobial silver nanoparticles by callus and leaf extract from salt marsh plants, Sesuvium portulacastrum L. Colloids Surf Biointerfaces 79:488–493

    Google Scholar 

  • Peleg AY, Hooper DC (2010) Hospital-acquired infections due to gram-negative bacteria. N Engl J Med 362(19):1804–1813

    CAS  PubMed  PubMed Central  Google Scholar 

  • Prabhu S, Poulose EK (2012) Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int Nano Lett 2:32

    Google Scholar 

  • Prasannaraj G, Venkatachalam P (2017) Green engineering of biomolecule-coated metallic silver nanoparticles and their potential cytotoxic activity against cancer cell lines. Adv Nat Sci Nanosci Nanotechnol. 8:025001

    Google Scholar 

  • Priyaragini S, Sathishkumar SR, Bhaskararao KV (2013) Biosynthesis of silver nanoparticles using actinobacteria and evaluating its antimicrobial and cytotoxicity activity. Int J Pharm Pharm Sci. 5(2):709–712

    CAS  Google Scholar 

  • Rajendran R, Ganesan N, Balu S, Alagar S, Thandavamoorthy P, Thiruvengadam D (2015) Green synthesis, characterization, antimicrobial and cytotoxic effects of silver nanoparticles using Origanum heracleoticum L. leaf extract. Int J Pharm Pharm Sci. 7(4):288–293

    CAS  Google Scholar 

  • Rajkumar G, Abdul Rahuman A (2011) Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vector. Acta Trop 118:196–203

    Google Scholar 

  • Raju K, Balaraman R, Hariprasad A, Vinoth Kumar M, Ali A (2008) Cardioprotective effect of Momordica cymbalaria Fenzl in rats with Isoproterenol-induced myocardial injury. J Clin Diagn Res 2(1):699–705

    Google Scholar 

  • Rao BK, Kesavulu MM, Giri R, Appa Rao C (1999) Anti-diabetic and hypolipidemic effects of Momordica cymbalaria Hook. fruit powder in alloxan-diabetic rats. J Ethnopharmacol 67(1):103–109

    CAS  PubMed  Google Scholar 

  • Ray PC, Yu H, Fu PP (2009) Toxicity and environmental risks of nanomaterials: challenges and future needs. J Environ Sci Health Part C Environ Carcinog Ecotoxicol Rev 27(1):1–35

    CAS  Google Scholar 

  • Ruparelia JP, Chatterjee A, Duttagupta SP, Mukherji S (2008) Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater 2008(4):707–716

    Google Scholar 

  • Sargazi G, Afzali D, Mostafavi A, Ebrahimipou SY (2017) Ultrasound-assisted facile synthesis of a new tantalum (V). Metal-organic framework nanostructure: design, characterization, systematic study, and CO2 adsorption performance. J Solid State Chem. https://doi.org/10.1016/j.jssc.2017.03.014

    Article  Google Scholar 

  • Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS (2009) Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf B Biointerfaces. 73(2):332–338

    CAS  PubMed  Google Scholar 

  • Shah MSAS, Nag M, Kalagara T, Singh S, Manorama SV (2008) Silver on PEG-PU-TiO2 Polymer Nanocomposite Films: An Excellent System for Antibacterial Applications. Chem Mater 20:2455–2460

    CAS  Google Scholar 

  • Shah RK, Boruah F, Parween N (2015) Synthesis and characterization of ZnO nanoparticles using leaf extract of Camellia sinensis and evaluation of their antimicrobial efficacy. Int J Curr Microbiol App Sci 4(8):444–450

    CAS  Google Scholar 

  • Sharmila G, Muthukumaran C, Sandiya K, Santhiya S, Sakthi Pradeep R, Manoj Kumar N, Suriyanarayanan N, Thirumarimurugan M (2018) Biosynthesis, characterization, and antibacterial activity of zinc oxide nanoparticles derived from Bauhinia tomentosa leaf extract. J Nanostruct Chem 8:293–299

    CAS  Google Scholar 

  • Siegel RL, Miller KD, Jemal A (2016) Cancer statistics. CA Cancer J Clin 66(1):7–30

    PubMed  Google Scholar 

  • Singh P, Chaturvedi A (2015) Complementary and alternative medicine in cancer pain management: a systematic review. Indian J Palliat Care 21(1):105–115

    PubMed  PubMed Central  Google Scholar 

  • Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP (2011) Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanoparticle Res 13:2981–2988

    CAS  Google Scholar 

  • Sun W, Zhai X, Zhao L (2016) Synthesis of ZIF-8 and ZIF-67 nanocrystals with well controllable size distribution through reverse microemulsions. Chem Eng J 289:59–64

    CAS  Google Scholar 

  • Teoh F, Pavelka N (2016) How chemotherapy increases the risk of systemic candidiasis in cancer patients: current paradigm and future directions. Pathogens 5(1):6

    PubMed Central  Google Scholar 

  • Viet Quang D, Hoai Chau N (2013) The effect of hydrothermal treatment on silver nanoparticles stabilized by chitosan and its possible application to produce mesoporous silver powder. J Powder Technol 2013:1–6

    Google Scholar 

  • Wachi K, Tateda K, Yamashiro Y, Takahashi M, Matsumoto T, Furuya N, Ishii Y, Akasaka Y, Yamaguchi K, Uchida K (2005) Sepsis caused by food-borne infection with Escherichia coli. Intern Med 44(12):1316–1319

    PubMed  Google Scholar 

  • Walker B, Mouton CP (2006) Nanotechnology and nanomedicine: a primer. J Natl Med Assoc 98(12):1985–1988

    PubMed  PubMed Central  Google Scholar 

  • Xu ZP, Zeng QH, Lu GQ, Yu AB (2006) Inorganic nanoparticles as carriers for efficient cellular delivery. Chem Eng Sci 61(3):1027–1040

    CAS  Google Scholar 

  • Zhang XF, Shen W, Gurunathan S (2016) Silver nanoparticle-mediated cellular responses in various cell lines: an in vitro model. Int J Mol Sci 17(10):1603

    PubMed Central  Google Scholar 

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Acknowledgements

The author great fully acknowledge the financial assistance from University Grants Commission, New Delhi, India under the scheme of UGC-Rajiv Gandhi National Fellowship.

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  1. Department of Biochemistry, Andhra University, Visakhapatnam, 530 003, India

    Hemalatha KPJ, Srinivasulu Shantakani & Satyanarayana Botcha

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KPJ, H., Shantakani, S. & Botcha, S. Green synthesis of silver nanoparticles using aqueous fruit and tuber extracts of Momordica cymbalaria. J. Plant Biochem. Biotechnol. 30, 196–204 (2021). https://doi.org/10.1007/s13562-019-00542-y

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