Abstract
In the present study, gold nanoparticles (CRnp) has been prepared using aqueous extract of Cyperus rotundus root at room temperature, sunlight, sonication, microwave oven heating and hot air oven method. Effect of concentration variation was studied using microwave-assisted synthesis. Grayish pink colour gold nanoparticles were formed within 5 s in microwave-assisted synthesis. Preliminary phytochemical screening showed the presence of alkaloids, protein and phenolic groups. These metabolites may be the responsible for the formation of gold nanoparticles. Reduced graphene oxide (CRrGO) was prepared using refluxing method and nano composite (CRNC) was prepared using equal ratio (1:1) of CRnp and CRrGO under homogenization method. The prepared CRnp, CRrGO and CRNC were characterized by spectroscopic techniques such as UV–visible spectroscopy, FTIR, XRD, Raman spectroscopy and FESEM. Thermal stability analysis of the CRrGO was carried out using TGA. FESEM results revealed the synthesized CRnp to be spherical in nature with average diameter 15 nm. CRrGO showed flake-like structures with few layers which was further confirmed by FESEM and Raman spectroscopy. The FESEM image of CRNC clearly portrays CRnp to be strongly bound to the surface of CRrGO. Anti-bacterial activity of the synthesized CRnp, CRrGO, CRNC and CRa synthesized nanoparticles to be active against gram negative and gram positive bacteria.
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Abbreviations
- CRnp:
-
Cyperus rotundus gold nanoparticles
- CRNC:
-
Cyperus rotundus nanocomposites
- CRrGO:
-
Cyperus rotundus reduced graphene oxide
- CRa:
-
Cyperus rotundus aqueous extract
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Acknowledgements
The authors are sincerely thankful to the Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamilnadu for providing research facilities. We thank Tamilnadu Agricultural University, Coimbatore for recording Raman spectroscopy.
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Sasidharan, S., Pottail, L. Antimicrobial activity of metal and non-metallic nanoparticles from Cyperus rotundus root extract on infectious disease causing pathogens. J. Plant Biochem. Biotechnol. 29, 134–143 (2020). https://doi.org/10.1007/s13562-019-00523-1
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DOI: https://doi.org/10.1007/s13562-019-00523-1