Molecular genetic diversity analysis for heat tolerance of indigenous and exotic wheat genotypes

Abstract

Four hundred and ninety-six indigenous and exotic aestivum and durum wheat accessions were phenotyped for heat tolerance at three locations during 2013–2014 and 2014–2015 crop seasons under very late sown conditions (35–40 °C). Promising 47 heat tolerant accessions, including 15 of durum were identified. Phenotypic data were recorded for quantitative traits and genotypic using molecular markers in these 47 lines. Polymorphism of 73.7% and 71.5% was detected using 137 and 109 SSR markers in aestivum and durum accessions respectively. In Triticum aestivum 308 alleles and in T. durum 234 alleles were detected with a range of 2–6. The genetic diversity ranged from 0.099 to 0.733 with average 0.488. Genotypes were classified into four categories based on their tolerance to stress as determined from Heat susceptibility index for grain yield. Dendrograms constructed using phenological, physiological and grain traits as well as molecular data were different with respect to alignment of genotypes indicating that genotypes differed genetically as well as physiologically in heat tolerance. Moreover phylogenetic tree is better choice as compared to population structure. The wheat breeders can pyramid these diverse sources to improve heat tolerance.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3

Abbreviations

CFL:

Chlorophyll fluorescence

CMS:

Cell membrane stability

HSI:

Heat susceptibility index

References

  1. Abouzied HM, Eldemery SMM, Abdellatif KF (2013) SSR-based genetic diversity assessement in tetraploid and hexaploid wheat populations. Br Biotechnol J 3:390–404

    Article  Google Scholar 

  2. Anderson JA, Churchill GA, Autrique JE, Tanksley SD, Sorrells ME (1993) Optimizing parental selection for genetic linkage maps. Genome 36:181–186

    CAS  Article  Google Scholar 

  3. Asseng S, Ewert F, Martre P, Rötter RP, Lobell DB, Cammarano D (2015) Rising temperatures reduce global wheat production. Nat Clim Change 5:143–147

    Article  Google Scholar 

  4. Bhusal N (2016) Genetic and molecular marker analysis for heat tolerance in wheat (Triticum aestivum L. em Thell) Ph.D. thesis submitted to CCS Haryana Agricultural University, Hisar

  5. Bhusal N, Sarial AK, Sharma P, Sareen S (2017) Mapping QTLs for grain yield components in wheat under heat stress. PLoS ONE 12(12):e0189594

    Article  Google Scholar 

  6. Bhusal N, Sharma P, Sareen S, Sarial AK (2018) Mapping QTLs for chlorophyll content and chlorophyll fluorescence in wheat under heat stress. Biol Plant 62(4):721–731

    CAS  Article  Google Scholar 

  7. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Chao S, Zhang W, Akhunov E, Sherman J, Ma Y, Luo MC, Dubcovsky J (2009) Analysis of gene-derived SNP marker polymorphism in US wheat (Triticum aestivum L.) cultivars. Mol Breed 23(1):23–33

    CAS  Article  Google Scholar 

  9. Dodig D, Quarrie S, Stanković S Milijić, Denčić S (2010) Characterizing wheat genetic resources for responses to drought stress. http://www.wg-crop.icidonline.org/38doc.pdf. Accessed 10 Mar 2017

  10. Evanno G, Regnaut S, Gould J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620

    CAS  Article  Google Scholar 

  11. Fischer RA (1985) Number of kernels in wheat crops and the influence of solar radiation and temperature. J Agric Sci 105:447–461

    Article  Google Scholar 

  12. Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars I Grain yield response. Aust J Agric Res 29:897–907

    Article  Google Scholar 

  13. Garg D, Sareen S, Dalal S, Tiwari R, Singh R (2012) Heat shock protein based SNP marker for terminal heat stress in wheat (Triticum aestivum L.). Aust J Crop Sci 6(11):1516–1521

    CAS  Google Scholar 

  14. Hede AR, Skovmand B, Reynolds MP, Crossa J, Vilhelmsen AL, Stølen O (1999) Evaluating genetic diversity for heat tolerance traits in Mexican wheat landraces. Genet Resour Crop Evol 46:37–45

    Article  Google Scholar 

  15. Kumar S, Archak S, Tyagi RK, Kumar J, Vikas VK, Jacob SR et al (2016) Evaluation of 19,460 wheat accessions conserved in the Indian National Genebank to identify new sources of resistance to rust and spot blotch diseases. PLoS ONE 11(12):e0167702 pmid:27942031

    Article  Google Scholar 

  16. Liu KJ, Muse SV (2005) Power Marker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21(9):2128–2129

    CAS  Article  Google Scholar 

  17. Mason AS (2015) SSR Genotyping. In: Batley J (ed) Plant genotyping. Methods in molecular biology (methods and protocols), vol 1245. Humana Press, New York, pp 77–89

    Google Scholar 

  18. Mondal S, Singh RP, Crossa J, Huerta-Espino J, Sharma I, Chatrath R, Singh GP, Sohu VS, Mavi GS, Sukuru VSP, Kalappanavar IK, Mishra VK, Hussain M, Gautam NR, Uddin J, Barma NCD, Hakim A, Joshi AK (2013) Earliness in wheat: a key to adaptation under terminal and continual high temperature stress in South Asia. Field Crop Res 151:19–26

    Article  Google Scholar 

  19. Mondal S, Singh RP, Mason ER, Huerta-Espino J, Autrique E, Joshi AK (2016) Grain yield, adaptation and progress in breeding for early-maturing and heat-tolerant wheat lines in South Asia. Field Crop Res 192:78–85

    CAS  Article  Google Scholar 

  20. Okechukwu CE, Agbo UC, Uguru IM, Ogbonnaya CF (2016) Germplasm evaluation of heat tolerance in bread wheat in Tel Hadya, Syria. Chil J Agric Res 76(1):9–17

    Article  Google Scholar 

  21. Pagnotta MA, Mondini L, Atallah MF (2005) Morphological and molecular characterization of Italian emmer wheat accessions. Euphytica 146:29–37

    Article  Google Scholar 

  22. Perrier X, Jacquemoud-Collet JP (2006) DARwin software CIRAD (French Agricultural Research Centre for International Development). http://darwin.cirad.fr/darwin

  23. Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK (2000) The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor Appl Genet 100:584–592

    CAS  Google Scholar 

  24. Rai N, Bellundagi A, Kumar PKC, Ramya KT et al (2018) Marker-assisted backcross breeding for improvement of drought tolerance in bread wheat. Plant Breed. https://doi.org/10.1111/pbr.12605

    Article  Google Scholar 

  25. Salunkhe A, Tamhankar S, Tetali S, Zaharieva M, Bonnett D, Trethowan R (2013) Molecular genetic diversity analysis in emmer wheat (Triticum dicoccon Schrank) from India. Genet Res Crop Evol 60:165–174

    CAS  Article  Google Scholar 

  26. Sareen S, Munjal R, Singh NB, Singh BN, Verma RS, Meena BK, Shoran J, Sarial AK, Singh SS (2012) Genotype x environment interaction and AMMI analysis for heat tolerance in wheat. Cereal Res Commun 40(2):267–276

    Article  Google Scholar 

  27. Sareen S, Tyagi BS, Sarial AK, Tiwari V, Sharma I (2014) Trait analysis, diversity, and genotype x environment interaction in some wheat landraces evaluated under drought and heat stress conditions. Chil J Agric Res 74:135–142

    Article  Google Scholar 

  28. Saxena DC, Sai Prasad SV, Parashar R, Rathi I (2016) Phenotypic characterization of specific adaptive physiological traits for heat tolerance in wheat. Indian J Plant Physiol 21:318–322

    Article  Google Scholar 

  29. Sharma P, Sareen S, Saini M, Shefali (2016). Assessing genetic variation for heat stress tolerance in Indian bread wheat genotypes using morpho-physiological traits and molecular markers. Plant Genet Resour. Available on CJO2016. https://doi.org/10.1017/s1479262116000241

    Article  Google Scholar 

  30. Shpiler L, Blum A (1990) Heat tolerance for yield and its components in different wheat cultivars. Euphytica 51(3):257–263

    Article  Google Scholar 

  31. Singh N, Choudhury DR, Singh AK, Kumar S, Srinivasan K, Tyagi RK, Singh NK, Singh R (2013) Comparison of SSR and SNP markers in estimation of genetic diversity and population structure of Indian rice varieties. PLoS ONE 8(12):e84136

    Article  Google Scholar 

  32. Teklu Y, Hammer K, Huang XQ, Roder MS (2006) Analysis of microsatellite diversity in Ethiopian tetraploid wheat landraces. Genet Resour Crop Evol 53:1115–1126

    CAS  Article  Google Scholar 

  33. Varshney RK, Chabane K, Hendre PS, Aggarwal RK, Graner A (2007) Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Crop Sci 173:638–649

    CAS  Google Scholar 

  34. Vieira ML, Santini L, Diniz AL, Munhoz CDF (2016) Microsatellite markers: what they mean and why they are so useful. Genet Mol Biol 39:312–328

    Article  Google Scholar 

Download references

Acknowledgements

Financial assistance from USAID through CIMMYT, Mexico under ARCADIA—CIMMYT—USAID project Development of heat tolerant wheat for South Asia (Grant No. OAA-A-13-00001) is acknowledged. Financial assistance from Indian Council of Agricultural Research under project Development of high yielding heat tolerant wheat cultivars by utilizing molecular and physiological resources (Project no. 1007689) is acknowledged.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Sindhu Sareen.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 1270 kb)

Supplementary material 2 (DOCX 37 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sareen, S., Bhusal, N., Kumar, M. et al. Molecular genetic diversity analysis for heat tolerance of indigenous and exotic wheat genotypes. J. Plant Biochem. Biotechnol. 29, 15–23 (2020). https://doi.org/10.1007/s13562-019-00501-7

Download citation

Keywords

  • Genetic diversity
  • SSR markers
  • Cell membrane stability
  • Chlorophyll fluorescence
  • Wheat