Four hundred and ninety-six indigenous and exotic aestivum and durum wheat accessions were phenotyped for heat tolerance at three locations during 2013–2014 and 2014–2015 crop seasons under very late sown conditions (35–40 °C). Promising 47 heat tolerant accessions, including 15 of durum were identified. Phenotypic data were recorded for quantitative traits and genotypic using molecular markers in these 47 lines. Polymorphism of 73.7% and 71.5% was detected using 137 and 109 SSR markers in aestivum and durum accessions respectively. In Triticum aestivum 308 alleles and in T. durum 234 alleles were detected with a range of 2–6. The genetic diversity ranged from 0.099 to 0.733 with average 0.488. Genotypes were classified into four categories based on their tolerance to stress as determined from Heat susceptibility index for grain yield. Dendrograms constructed using phenological, physiological and grain traits as well as molecular data were different with respect to alignment of genotypes indicating that genotypes differed genetically as well as physiologically in heat tolerance. Moreover phylogenetic tree is better choice as compared to population structure. The wheat breeders can pyramid these diverse sources to improve heat tolerance.
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Cell membrane stability
Heat susceptibility index
Abouzied HM, Eldemery SMM, Abdellatif KF (2013) SSR-based genetic diversity assessement in tetraploid and hexaploid wheat populations. Br Biotechnol J 3:390–404
Anderson JA, Churchill GA, Autrique JE, Tanksley SD, Sorrells ME (1993) Optimizing parental selection for genetic linkage maps. Genome 36:181–186
Asseng S, Ewert F, Martre P, Rötter RP, Lobell DB, Cammarano D (2015) Rising temperatures reduce global wheat production. Nat Clim Change 5:143–147
Bhusal N (2016) Genetic and molecular marker analysis for heat tolerance in wheat (Triticum aestivum L. em Thell) Ph.D. thesis submitted to CCS Haryana Agricultural University, Hisar
Bhusal N, Sarial AK, Sharma P, Sareen S (2017) Mapping QTLs for grain yield components in wheat under heat stress. PLoS ONE 12(12):e0189594
Bhusal N, Sharma P, Sareen S, Sarial AK (2018) Mapping QTLs for chlorophyll content and chlorophyll fluorescence in wheat under heat stress. Biol Plant 62(4):721–731
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
Chao S, Zhang W, Akhunov E, Sherman J, Ma Y, Luo MC, Dubcovsky J (2009) Analysis of gene-derived SNP marker polymorphism in US wheat (Triticum aestivum L.) cultivars. Mol Breed 23(1):23–33
Dodig D, Quarrie S, Stanković S Milijić, Denčić S (2010) Characterizing wheat genetic resources for responses to drought stress. http://www.wg-crop.icidonline.org/38doc.pdf. Accessed 10 Mar 2017
Evanno G, Regnaut S, Gould J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620
Fischer RA (1985) Number of kernels in wheat crops and the influence of solar radiation and temperature. J Agric Sci 105:447–461
Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars I Grain yield response. Aust J Agric Res 29:897–907
Garg D, Sareen S, Dalal S, Tiwari R, Singh R (2012) Heat shock protein based SNP marker for terminal heat stress in wheat (Triticum aestivum L.). Aust J Crop Sci 6(11):1516–1521
Hede AR, Skovmand B, Reynolds MP, Crossa J, Vilhelmsen AL, Stølen O (1999) Evaluating genetic diversity for heat tolerance traits in Mexican wheat landraces. Genet Resour Crop Evol 46:37–45
Kumar S, Archak S, Tyagi RK, Kumar J, Vikas VK, Jacob SR et al (2016) Evaluation of 19,460 wheat accessions conserved in the Indian National Genebank to identify new sources of resistance to rust and spot blotch diseases. PLoS ONE 11(12):e0167702 pmid:27942031
Liu KJ, Muse SV (2005) Power Marker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21(9):2128–2129
Mason AS (2015) SSR Genotyping. In: Batley J (ed) Plant genotyping. Methods in molecular biology (methods and protocols), vol 1245. Humana Press, New York, pp 77–89
Mondal S, Singh RP, Crossa J, Huerta-Espino J, Sharma I, Chatrath R, Singh GP, Sohu VS, Mavi GS, Sukuru VSP, Kalappanavar IK, Mishra VK, Hussain M, Gautam NR, Uddin J, Barma NCD, Hakim A, Joshi AK (2013) Earliness in wheat: a key to adaptation under terminal and continual high temperature stress in South Asia. Field Crop Res 151:19–26
Mondal S, Singh RP, Mason ER, Huerta-Espino J, Autrique E, Joshi AK (2016) Grain yield, adaptation and progress in breeding for early-maturing and heat-tolerant wheat lines in South Asia. Field Crop Res 192:78–85
Okechukwu CE, Agbo UC, Uguru IM, Ogbonnaya CF (2016) Germplasm evaluation of heat tolerance in bread wheat in Tel Hadya, Syria. Chil J Agric Res 76(1):9–17
Pagnotta MA, Mondini L, Atallah MF (2005) Morphological and molecular characterization of Italian emmer wheat accessions. Euphytica 146:29–37
Perrier X, Jacquemoud-Collet JP (2006) DARwin software CIRAD (French Agricultural Research Centre for International Development). http://darwin.cirad.fr/darwin
Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK (2000) The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor Appl Genet 100:584–592
Rai N, Bellundagi A, Kumar PKC, Ramya KT et al (2018) Marker-assisted backcross breeding for improvement of drought tolerance in bread wheat. Plant Breed. https://doi.org/10.1111/pbr.12605
Salunkhe A, Tamhankar S, Tetali S, Zaharieva M, Bonnett D, Trethowan R (2013) Molecular genetic diversity analysis in emmer wheat (Triticum dicoccon Schrank) from India. Genet Res Crop Evol 60:165–174
Sareen S, Munjal R, Singh NB, Singh BN, Verma RS, Meena BK, Shoran J, Sarial AK, Singh SS (2012) Genotype x environment interaction and AMMI analysis for heat tolerance in wheat. Cereal Res Commun 40(2):267–276
Sareen S, Tyagi BS, Sarial AK, Tiwari V, Sharma I (2014) Trait analysis, diversity, and genotype x environment interaction in some wheat landraces evaluated under drought and heat stress conditions. Chil J Agric Res 74:135–142
Saxena DC, Sai Prasad SV, Parashar R, Rathi I (2016) Phenotypic characterization of specific adaptive physiological traits for heat tolerance in wheat. Indian J Plant Physiol 21:318–322
Sharma P, Sareen S, Saini M, Shefali (2016). Assessing genetic variation for heat stress tolerance in Indian bread wheat genotypes using morpho-physiological traits and molecular markers. Plant Genet Resour. Available on CJO2016. https://doi.org/10.1017/s1479262116000241
Shpiler L, Blum A (1990) Heat tolerance for yield and its components in different wheat cultivars. Euphytica 51(3):257–263
Singh N, Choudhury DR, Singh AK, Kumar S, Srinivasan K, Tyagi RK, Singh NK, Singh R (2013) Comparison of SSR and SNP markers in estimation of genetic diversity and population structure of Indian rice varieties. PLoS ONE 8(12):e84136
Teklu Y, Hammer K, Huang XQ, Roder MS (2006) Analysis of microsatellite diversity in Ethiopian tetraploid wheat landraces. Genet Resour Crop Evol 53:1115–1126
Varshney RK, Chabane K, Hendre PS, Aggarwal RK, Graner A (2007) Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Crop Sci 173:638–649
Vieira ML, Santini L, Diniz AL, Munhoz CDF (2016) Microsatellite markers: what they mean and why they are so useful. Genet Mol Biol 39:312–328
Financial assistance from USAID through CIMMYT, Mexico under ARCADIA—CIMMYT—USAID project Development of heat tolerant wheat for South Asia (Grant No. OAA-A-13-00001) is acknowledged. Financial assistance from Indian Council of Agricultural Research under project Development of high yielding heat tolerant wheat cultivars by utilizing molecular and physiological resources (Project no. 1007689) is acknowledged.
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Sareen, S., Bhusal, N., Kumar, M. et al. Molecular genetic diversity analysis for heat tolerance of indigenous and exotic wheat genotypes. J. Plant Biochem. Biotechnol. 29, 15–23 (2020). https://doi.org/10.1007/s13562-019-00501-7
- Genetic diversity
- SSR markers
- Cell membrane stability
- Chlorophyll fluorescence