Phytic acid or phytate (myo-inositol hexakisphosphate) is the principal storage indigestible form of phosphorus in different crops. It is considered as an antinutrient in human as well as animal (including fish, poultry, pig, chicken etc.) diet due to its chelating behavior of certain essential divalent minerals (Fe2+, Mg2+, Zn2+, Ca2+ etc.). The unabsorbed, indigested form of phosphorus also causes phosphate pollution in the soil by animal wastes. Phytate degrading enzymes like phytases (myo-inositol hexakisphosphate phosphohydrolase) in this regard can be very useful and also economically feasible to reduce the risk of phosphate pollution and increase the nutrient value in animal feeds at the same time. The Klebsiella phytases are suitable to use in the food industries of plant origin for their excellent thermal stability and high pH tolerance. From the present in silico investigation, it was found that Klebsiella phytases were 46–47 kDa molecular weight protein of histidine phosphatase superfamily having thermostability and alkalinity nature. This thermostability can be achieved due to possession of higher percentage of α helices and β sheets at the same time; the presence of higher aliphatic indices (range in between 88 and 91) etc. Interestingly, a strong correlation was found to be pertinent from phylogenetic studies of proteins with their cDNA among both species and strain level. Hence, the present study would be beneficial for future researchers (3D model available in Protein Model Database with acc. no.: PM0080562) to meet the demand of agricultural and industrial production of bacterial phytases particularly for agricultural farming.
Escobin-Mopera L, Ohtani M, Sekiguchi S et al (2012) Purification and characterization of phytase from Klebsiella pneumoniae 9-3B. J Biosci Bioeng 113:562–567CrossRefPubMedGoogle Scholar
Gasteiger E, Hoogland C, Gattiker A et al (2005) Protein identification and analysis tools on the ExPASy server. In: Walker JM (ed) The proteomics protocols handbook. Humana Press, New York, pp 571–607CrossRefGoogle Scholar
Gontia-Mishra I, Tiwari S (2013) Phylogenetic analysis of fungal phytases. Food Technol Biotechnol 51:313–326Google Scholar
Gontia-Mishra I, Singh VK, Tripathi N et al (2014) Computational identification, homology modelling and docking analysis of phytase protein from Fusarium oxysporum. Biologia 69:1283–1294CrossRefGoogle Scholar
Verma A, Singh VK, Gaur S (2016) Computational based functional analysis of Bacillus phytases. Comput Biol Chem 60:53–58CrossRefPubMedGoogle Scholar
Wyss M, Brugger R, Kronenberger A et al (1999) Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. Appl Environ Microbiol 65:367–373PubMedPubMedCentralGoogle Scholar