In silico structural, functional and phylogenetic analysis of Klebsiella phytases

  • Krishnendu Pramanik
  • Priyanka Pal
  • Tithi Soren
  • Soumik Mitra
  • Pallab Kumar Ghosh
  • Anumita Sarkar
  • Tushar Kanti Maiti
Original Article
  • 30 Downloads

Abstract

Phytic acid or phytate (myo-inositol hexakisphosphate) is the principal storage indigestible form of phosphorus in different crops. It is considered as an antinutrient in human as well as animal (including fish, poultry, pig, chicken etc.) diet due to its chelating behavior of certain essential divalent minerals (Fe2+, Mg2+, Zn2+, Ca2+ etc.). The unabsorbed, indigested form of phosphorus also causes phosphate pollution in the soil by animal wastes. Phytate degrading enzymes like phytases (myo-inositol hexakisphosphate phosphohydrolase) in this regard can be very useful and also economically feasible to reduce the risk of phosphate pollution and increase the nutrient value in animal feeds at the same time. The Klebsiella phytases are suitable to use in the food industries of plant origin for their excellent thermal stability and high pH tolerance. From the present in silico investigation, it was found that Klebsiella phytases were 46–47 kDa molecular weight protein of histidine phosphatase superfamily having thermostability and alkalinity nature. This thermostability can be achieved due to possession of higher percentage of α helices and β sheets at the same time; the presence of higher aliphatic indices (range in between 88 and 91) etc. Interestingly, a strong correlation was found to be pertinent from phylogenetic studies of proteins with their cDNA among both species and strain level. Hence, the present study would be beneficial for future researchers (3D model available in Protein Model Database with acc. no.: PM0080562) to meet the demand of agricultural and industrial production of bacterial phytases particularly for agricultural farming.

Keywords

Klebsiella sp. Phylogenetic comparison In silico analysis Alkaline phytase Histidine phosphatase superfamily 

Abbreviations

UniProt KB

UniProt knowledgebase

UniParc

UniProt archive

BLAST

Basic local alignment search tool

MEGA

Molecular evolutionary genetics analysis

EMBL

The European Molecular Biology Laboratory

QMEAN

Qualitative Model Energy ANalysis

Notes

Acknowledgements

Authors are thankful to Department of Science and Technology, New Delhi, Government of India for financial assistance to the first author (INSPIRE Fellow Reg. No. IF150197).

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

Supplementary material

13562_2018_445_MOESM1_ESM.docx (453 kb)
Supplementary material 1 (DOCX 453 kb)

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2018

Authors and Affiliations

  • Krishnendu Pramanik
    • 1
  • Priyanka Pal
    • 1
  • Tithi Soren
    • 1
  • Soumik Mitra
    • 1
  • Pallab Kumar Ghosh
    • 1
  • Anumita Sarkar
    • 1
  • Tushar Kanti Maiti
    • 1
  1. 1.Microbiology Laboratory, Department of BotanyBurdwan UniversityPurba BardhamanIndia

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