Extraction, purification and characterization of a novel cysteine protease from the latex of plant Vallaris solanacea
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Abstract
Plant proteases with excellent catalytical properties perform many functions in biological systems. A novel plant protease Vallaris solanacea, was identified. Its proteolytic activity was screened using the substrate casein. This protein activity was specifically inhibited by p-chloromercuribenzoate, which showed that it is a cysteine protease. Preliminary investigations such as pH effect and temperature dependence on the caseinolytic activity of crude protease were done. Stability towards temperature and pH were also evaluated. The activity curves drawn in relation to pH, temperature and stability suggested the presence of one protease in the latex of Vallaris solanacea. In the present study, separation and purification of the latex cysteine protease solanain from Vallaris solanacea to a state of near homogeneity was also done using ion exchange and size exclusion chromatography. SDS PAGE was used to determine molecular weight of the solanain (28–29 kDa). The molecular weight was confirmed as 28.9 kDa using MALDI-TOF. Purified protease was named solanain and it was further characterized. An internal tryptic fragment was identified by MALDI-TOF, and this peptide showed a homology (66% sequence similarity) with target sequence of cysteine endopeptidase from Ricinus communis.
Keywords
Latex Cysteine proteases Vallaris solanacea Caseinolytic activity Crude protease Sequence identity Ricinus communisAbbreviations
- β-ME
β-Mercapto ethanol
- TCA
Tri chloro acetate
- EDTA
Ethylene diamino tetra acetic acid
- PCMB
p-Chloromercuribenzoate
- SDS
Sodium dodecyl sulphate
- DEAE
Diethyl amino ethyl
- PMSF
Phenyl methyl sulfonyl fluoride
- FC
Folin–Ciocalteu reagent
Notes
Acknowledgements
We thank the Department of Biochemistry, Gandhi Institute of Technology and Management, for providing the necessary facilities to conduct our research.
Compliance with ethical standards
Conflict of interest
The authors have no conflicts of interest.
Ethical approval
The article is entirely a study on plants. It does not include any animals or human participants.
Supplementary material
References
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