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Biology of B. sorokiniana (syn. Cochliobolus sativus) in genomics era

  • Pushpendra K. Gupta
  • Neeraj K. Vasistha
  • Rashmi Aggarwal
  • Arun K. Joshi
Review Article
  • 150 Downloads

Abstract

Bipolaris sorokiniana (Sacc.) Shoemaker is a hemi-biotrophic fungal pathogen, which is an anamorph (teleomorph Cochlibolus sativus). It causes spot blotch, root rot and leaf spot diseases in a number of cereals including wheat, barley and other small grain cereals. In the genomics era, the fungus has been subjected to a variety of studies using molecular approaches. Correct chromosome number was determined and molecular karyotypes were prepared using contour-clamped homogeneous electric field. Molecular maps were prepared using markers like RFLPs, SSRs, RAPDs and SNPs. For this purpose, segregating progenies derived from crosses between diverse isolates of the pathogen were used. Whole genome sequencing (WGS) data was collected not only for B. sorokiniana isolates, but also for several species of Cochliobolus. Genes involved in secondary metabolism and virulence were identified from genome sequences. The WGS data has also been utilized for comparative genomics giving useful information about evolutionary trends. A brief account of this information is presented in this review.

Keywords

Spot blotch Bipolaris sorokiniana Karyotypes NRPSs VHv1 Genomics 

Abbreviations

SB

Spot blotch

CRR

Common root rot

ITS

Internal transcribed spacer

PTI

PAMP-triggered immunity

ETI

Effector-triggered immunity

ETS

Effector triggered susceptibility

NRPS

Non-ribosomal peptide synthetases

PKS

Polyketide synthases

NGS

Next generation sequencing

NBS LRR

Nucleotide-binding site leucine-rich repeat

S/TPK

Serine/threonine protein kinase

NE

Necrotrophic effector

CHEF

Contour-clamped homogeneous electric field

GTBM

Germ tube burst method

AFLP

Amplified fragment length polymorphism

HST

Host-selective toxin

Avr

Avirulence

NLS

Nuclear localization signals

SSP

Small secreted protein

EST

Expressed sequence tag

SSR

Simple sequence repeat

qRT-PCR

Quantitative real-time polymerase chain reaction

Notes

Acknowledgements

PKG was awarded a Senior Scientist position by Indian National Science Academy (INSA), during the tenure of which this study was undertaken. Thanks are due to Head, Department of Genetics and Plant Breeding, CCS University, Meerut for providing facilities and to the Department of Science and Technology, Government of India for the award of a Young Scientist Project to NKV, which facilitated this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2017

Authors and Affiliations

  • Pushpendra K. Gupta
    • 1
  • Neeraj K. Vasistha
    • 1
  • Rashmi Aggarwal
    • 2
  • Arun K. Joshi
    • 3
  1. 1.Molecular Biology Laboratory, Department of Genetics and Plant BreedingCh. Charan Singh UniversityMeerutIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia
  3. 3.The International Maize and Wheat Improvement Center (CIMMYT)New DelhiIndia

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