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Utility of SSR markers in assessing the purity and identity of pearl millet hybrids

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Abstract

In the present study, 30 pearl millet genotypes comprising 11 hybrids and their parental lines were used to examine the suitability of SSR markers for ensuring genetic purity and identity of seed lots. A set of nine informative SSR markers were identified that could be effectively used for the assessment of genetic purity of ten public sector-bred commercial pearl millet hybrids. Among the identified markers, five markers differentiated six hybrids from each other. Highly uniform SSR allelic profile was confirmed through the analysis of plant-to-plant variation within the parental lines of the hybrids using the identified markers, which further strengthens the application of these markers in maintenance and purity testing of hybrids and parental lines. The utility of identified markers for the genetic purity testing of pearl millet hybrids was demonstrated using two commercial seed lots of the hybrid, RHRBH8609 and HHB67, which revealed a contamination of A and/or B line to the extent of 3–4 %.

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Fig. 1

Abbreviations

RAPD:

Random amplified polymorphic DNA

SSR:

Simple sequence repeat

DUS:

Distinctness, uniformity and stability

CMS:

Cytoplasmic male sterility

GOT:

Grow out test

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Acknowledgments

The first author is thankful to the ICAR (Indian Council of Agricultural Research, New Delhi, India) for the grant of junior research fellowship for the master degree programme. Authors are also thankful to the respective breeders of the pearl millet hybrids for providing genetically pure seeds.

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Authors and Affiliations

  1. Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Sanjay Kumar, Malavika Dadlani & M.B. Arun Kumar

  2. Division of Germplasm Conservation, National Bureau of Plant Genetic Resources, New Delhi, 110012, India

    Sherry Rachel Jacob

  3. Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110012, India

    C. Tara Satyavathi

Authors
  1. Sanjay Kumar
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  2. Sherry Rachel Jacob
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  3. C. Tara Satyavathi
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  4. Malavika Dadlani
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  5. M.B. Arun Kumar
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Corresponding author

Correspondence to M.B. Arun Kumar.

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Kumar, S., Jacob, S.R., Tara Satyavathi, C. et al. Utility of SSR markers in assessing the purity and identity of pearl millet hybrids. J. Plant Biochem. Biotechnol. 25, 121–124 (2016). https://doi.org/10.1007/s13562-015-0329-3

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  • DOI: https://doi.org/10.1007/s13562-015-0329-3

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