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Metageographic population analysis of Colletotrichum truncatum associated with chili fruit rot and other hosts using ITS region nucleotide sequences

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Abstract

Colletotrichum truncatum is one of the most economically important fungal pathogen causing anthracnose disease in pre and post-harvest stages of many crops worldwide. Little information is available in the literature on the genetic analysis and demographic history of this fungal pathogen. In the present study nucleotide sequence data of internal transcribed spacer (ITS) region were analyzed for C. truncatum isolates infecting chili and other crops worldwide to determine a metageographic pattern of distribution and evolution of the species. Levels of differentiation (genetic distances and F ST values) among sequences of C. truncatum from 23 countries were minimal suggesting the global occurrence of a large and geographically undifferentiated population. Only 11 haplotypes were detected among 98 isolates from 24 geographically distant populations of C. truncatum. Predominant haplotype H1 which occupied a central position in the median joining network was inferred to be ancestral haplotype as it was detected at a high frequency and was shared by multiple populations. Phylogeographic pattern of the species with worldwide presence and predominance of single haplotype suggests human mediated dispersal through domestication and introduction of host plants in different parts of the world, and might have played a significant role in structuring the populations of this devastating pathogen.

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Abbreviations

rDNA:

Ribosomal deoxyribonucleic acid

ITS:

Internal transcribed spacer

MEGA:

Molecular evolutionary genetics analysis

cTAB:

Cetyl trimethylammonium bromide

DNA:

Deoxyribonucleic acid

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Acknowledgments

The corresponding author is grateful to the Department of Science and Technology, Govt. of India, for financial assistance. We would like to thank the anonymous reviewers for their critical comments and suggestions for the improvement of the manuscript.

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Author notes

  1. Chandra S. Prabhakar

    Present address: Department of Entomology, Bihar Agricultural University, Sabour, Bhagalpur, 813210, India

Authors and Affiliations

  1. Molecular Plant Pathology Laboratory, Department of Plant Pathology, CSK HP Agricultural University, Palampur, 176 062, Himachal Pradesh, India

    Abhishek Katoch & Prem N. Sharma

  2. Department of Entomology, CSK HP Agricultural University, Palampur, 176 062, Himachal Pradesh, India

    Chandra S. Prabhakar

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  1. Abhishek Katoch
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  2. Chandra S. Prabhakar
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Correspondence to Prem N. Sharma.

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Katoch, A., Prabhakar, C.S. & Sharma, P.N. Metageographic population analysis of Colletotrichum truncatum associated with chili fruit rot and other hosts using ITS region nucleotide sequences. J. Plant Biochem. Biotechnol. 25, 64–72 (2016). https://doi.org/10.1007/s13562-015-0310-1

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