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Responses of sweet potato peroxidases to sodium nitroprusside-mediated nitric oxide

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Abstract

To ascertain the response of sweetpotato peroxidases (PODs) to nitric oxide (NO), we treated the leaves of sweet potato with the NO generator sodium nitroprusside (SNP) and the NO scavenger carboxyl-PTIO (cPTIO). Exogenous application of more than 5 mM SNP caused damage to sweetpotato leaves at 24 h after treatment. The accumulation of NO in leaves was positively correlated with the SNP dose. The specific activity of PODs in sweet potato leaves was markedly increased by treatment with greater than 1 mM SNP for 24 h, whereas POD activity and accumulated NO content decreased to low levels by treatment with cPTIO. Expression analysis of POD genes in response to treatment with SNP and cPTIO revealed that major stress-inducible acidic genes, such as swpa1, swpa2, swpa3, and swpa4, were specifically regulated. These results indicate that increased NO levels in sweet potato leaves are closely linked to an improved defense capability mediated by stress-inducible PODs.

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Fig. 1
Fig. 2

Abbreviations

cPIO:

2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

POD:

Peroxidase

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

SNP:

Sodium nitroprusside

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Acknowledgments

This work was supported by grants from the National Center for GM Crops (PJ008097) and the Systems & Synthetic Agrobiotech Center (PJ008119), Biogreen21 Project for Next Generation, Rural Development Administration, Korea, and the KRIBB initiative program.

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Authors and Affiliations

  1. Department of Biology Education, College of Education, and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-Daero, Jinju, 660-701, Republic of Korea

    Yun-Hee Kim

  2. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yusong-gu, Daejeon, 305-806, Republic of Korea

    Jae Cheol Jeong, Haeng-Soon Lee & Sang-Soo Kwak

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  1. Yun-Hee Kim
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  2. Jae Cheol Jeong
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  3. Haeng-Soon Lee
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  4. Sang-Soo Kwak
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Correspondence to Sang-Soo Kwak.

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Kim, YH., Jeong, J.C., Lee, HS. et al. Responses of sweet potato peroxidases to sodium nitroprusside-mediated nitric oxide. J. Plant Biochem. Biotechnol. 23, 440–443 (2014). https://doi.org/10.1007/s13562-014-0272-8

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  • DOI: https://doi.org/10.1007/s13562-014-0272-8

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