Skip to main content

Association of an allele-specific marker with dehydration stress tolerance in foxtail millet suggests SiDREB2 to be an important QTL

Abstract

Dehydration-responsive element binding (DREB) genes assist in improving stress tolerance of plants by activating the expression of several stress-responsive genes. Therefore, development of functional markers for useful alleles utilizing DREB genes is crucial for crop improvement strategies. Earlier we reported a synonymous single nucleotide polymorphism (SNP) associated with dehydration tolerance at 558th bp (an A/G transition) in the SiDREB2 gene of foxtail millet (Setaria italica L.) and developed an allele-specific marker (ASM) for SiDREB2. In the present study, we validated this ASM using a set of 122 foxtail accessions, of which 45 were investigated in an earlier study. The QTL associated with SiDREB2 contributed to ~20 % of the total phenotypic variation (PV) for relative water content (RWC) and this signified the importance of this QTL for dehydration tolerance in foxtail millet.

This is a preview of subscription content, access via your institution.

Fig. 1

Abbreviations

DREB:

Dehydration Responsive Element Binding

SNP:

Single Nucleotide Polymorphism

ASM:

Allele-Specific Marker

SMR:

Single Marker Regression

RWC:

Relative Water Content

QTL:

Quantitative Trait Loci

PEG:

Poly Ethylene Glycol

LP:

Lipid Peroxidation

References

  • Anderson JR, Asp T, Lu YC, Kloiber-Maitz M, Ouzonova M, Luebberstedt T (2009) Development and mapping of gene-tagged SNP markers in laccases of maize (Zea mays L.). Plant Breed 128:423–425

    Article  Google Scholar 

  • Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aus J Biol Sci 15:413–428

    Google Scholar 

  • Garcés-Claver A, Fellman SM, Gil-Ortega R, Jahn M, Arnedo-Andrés MS (2007) Identification, validation and survey of a single nucleotide polymorphism (SNP) associated with pungency in Capsicum spp. Theor Appl Genet 115:907–916

    PubMed  Article  Google Scholar 

  • Hussain SS, Kayani MA, Amjad M (2011) Transcription factors as tools to engineer enhanced drought tolerance in plants. Am Inst Chem Eng 27:297–306

    Google Scholar 

  • Kearsey MJ, Hyne V (1994) QTL analysis: a simple marker regression approach. Theor Appl Genet 89:698–702

    CAS  PubMed  Article  Google Scholar 

  • Lata C, Sahu PP, Prasad M (2010) Comparative transcriptome analysis of differentially expressed genes in foxtail millet (Setaria italica L.) during dehydration stress. Biochem Biophys Res Commun 393:720–727

    CAS  PubMed  Article  Google Scholar 

  • Lata C, Bhutty S, Bahadur RP, Majee M, Prasad M (2011) Association of an SNP in a novel DREB2-like gene SiDREB2 with stress tolerance in foxtail millet [Setaria italica (L.)]. J Exp Bot 62:3387–3401

    CAS  PubMed  Article  Google Scholar 

  • Lata C, Prasad M (2012) Validation of an allele-specific marker associated with dehydration stress tolerance in a core set of foxtail millet accessions. Plant Breed. doi:10.1111/j.1439-0523.2012.01983.x

  • Lata C, Prasad M (2011) Role of DREBs in regulation of abiotic stress responses in plants. J Exp Bot 62:4731–4748

    CAS  PubMed  Article  Google Scholar 

  • Li YM (1997) Drought-resistant mechanism and genetic expression of foxtail millet. In: Li YM (ed) Foxtail millet breeding. Agricultural Press, Beijing, pp 433–434

    Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    CAS  PubMed  Google Scholar 

  • Price A, Steele K, Townend J, Gorham J, Audebert A, Jones M, Courtois B (1999) Mapping roots and shoot traits in rice: experience in UK, IRRI and WARDA. In: Ito O, O’Toole JC, Hardy B (eds) Genetic improvement of rice for water-limited environments. IRRI, Philippines, pp 257–273

    Google Scholar 

  • Rajalakshmi S, Parida A (2012) Halophytes as a source of genes for abiotic stress tolerance. J Plant Biochem Biotech 21:63–67

    CAS  Article  Google Scholar 

  • Smart RE, Bingham GE (1974) Rapid estimates of relative water content. Plant Physiol 53:258–260

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Witcombe JR, Hollington PA, Howarth CJ, Reader S, Steele KA (2008) Breeding for abiotic stresses for sustainable agriculture. Phil Trans R Soc B 363:703–716

    CAS  PubMed  Article  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273

    CAS  PubMed Central  PubMed  Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the Department of Biotechnology (Grant No. BT/PR9851/AGR/02/521/2007), Govt. of India, New Delhi and core grant from the National Institute of Plant Genome Research, New Delhi, India. Dr. Charu Lata acknowledges Department of Science & Technology, Govt. of India for the INSPIRE Faculty award [IFA-11LSPA-01]. We thank Dr. Swarup K Parida and Mr. Mehanathan Muthamilarasan, NIPGR, New Delhi for critically reading the manuscript. We are thankful to NBPGR, Hyderabad and UAS, GKVK, Bangalore, India for providing the seed materials.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Manoj Prasad.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table S1

Description of 122 foxtail millet accessions along with the level of RWC at control and 24 h duration of dehydration stress, respectively; their allelic information and group position obtained by structure analysis. (DOC 334 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lata, C., Prasad, M. Association of an allele-specific marker with dehydration stress tolerance in foxtail millet suggests SiDREB2 to be an important QTL. J. Plant Biochem. Biotechnol. 23, 119–122 (2014). https://doi.org/10.1007/s13562-013-0193-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13562-013-0193-y

Keywords

  • Allele-specific marker
  • Dehydration stress
  • Foxtail millet
  • Marker-aided breeding
  • SiDREB2
  • Single marker regression
  • Single nucleotide polymorphism