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Journal of Plant Biochemistry and Biotechnology

, Volume 23, Issue 1, pp 119–122 | Cite as

Association of an allele-specific marker with dehydration stress tolerance in foxtail millet suggests SiDREB2 to be an important QTL

  • Charu Lata
  • Manoj PrasadEmail author
Short Communication

Abstract

Dehydration-responsive element binding (DREB) genes assist in improving stress tolerance of plants by activating the expression of several stress-responsive genes. Therefore, development of functional markers for useful alleles utilizing DREB genes is crucial for crop improvement strategies. Earlier we reported a synonymous single nucleotide polymorphism (SNP) associated with dehydration tolerance at 558th bp (an A/G transition) in the SiDREB2 gene of foxtail millet (Setaria italica L.) and developed an allele-specific marker (ASM) for SiDREB2. In the present study, we validated this ASM using a set of 122 foxtail accessions, of which 45 were investigated in an earlier study. The QTL associated with SiDREB2 contributed to ~20 % of the total phenotypic variation (PV) for relative water content (RWC) and this signified the importance of this QTL for dehydration tolerance in foxtail millet.

Keywords

Allele-specific marker Dehydration stress Foxtail millet Marker-aided breeding SiDREB2 Single marker regression Single nucleotide polymorphism 

Abbreviations

DREB

Dehydration Responsive Element Binding

SNP

Single Nucleotide Polymorphism

ASM

Allele-Specific Marker

SMR

Single Marker Regression

RWC

Relative Water Content

QTL

Quantitative Trait Loci

PEG

Poly Ethylene Glycol

LP

Lipid Peroxidation

Notes

Acknowledgments

This study was supported by the Department of Biotechnology (Grant No. BT/PR9851/AGR/02/521/2007), Govt. of India, New Delhi and core grant from the National Institute of Plant Genome Research, New Delhi, India. Dr. Charu Lata acknowledges Department of Science & Technology, Govt. of India for the INSPIRE Faculty award [IFA-11LSPA-01]. We thank Dr. Swarup K Parida and Mr. Mehanathan Muthamilarasan, NIPGR, New Delhi for critically reading the manuscript. We are thankful to NBPGR, Hyderabad and UAS, GKVK, Bangalore, India for providing the seed materials.

Supplementary material

13562_2013_193_MOESM1_ESM.doc (334 kb)
Supplementary Table S1 Description of 122 foxtail millet accessions along with the level of RWC at control and 24 h duration of dehydration stress, respectively; their allelic information and group position obtained by structure analysis. (DOC 334 kb)

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2013

Authors and Affiliations

  1. 1.National Research Centre on Plant BiotechnologyNew DelhiIndia
  2. 2.National Institute of Plant Genome Research (NIPGR)New DelhiIndia

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