Association of an allele-specific marker with dehydration stress tolerance in foxtail millet suggests SiDREB2 to be an important QTL
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Dehydration-responsive element binding (DREB) genes assist in improving stress tolerance of plants by activating the expression of several stress-responsive genes. Therefore, development of functional markers for useful alleles utilizing DREB genes is crucial for crop improvement strategies. Earlier we reported a synonymous single nucleotide polymorphism (SNP) associated with dehydration tolerance at 558th bp (an A/G transition) in the SiDREB2 gene of foxtail millet (Setaria italica L.) and developed an allele-specific marker (ASM) for SiDREB2. In the present study, we validated this ASM using a set of 122 foxtail accessions, of which 45 were investigated in an earlier study. The QTL associated with SiDREB2 contributed to ~20 % of the total phenotypic variation (PV) for relative water content (RWC) and this signified the importance of this QTL for dehydration tolerance in foxtail millet.
KeywordsAllele-specific marker Dehydration stress Foxtail millet Marker-aided breeding SiDREB2 Single marker regression Single nucleotide polymorphism
Dehydration Responsive Element Binding
Single Nucleotide Polymorphism
Single Marker Regression
Relative Water Content
Quantitative Trait Loci
Poly Ethylene Glycol
This study was supported by the Department of Biotechnology (Grant No. BT/PR9851/AGR/02/521/2007), Govt. of India, New Delhi and core grant from the National Institute of Plant Genome Research, New Delhi, India. Dr. Charu Lata acknowledges Department of Science & Technology, Govt. of India for the INSPIRE Faculty award [IFA-11LSPA-01]. We thank Dr. Swarup K Parida and Mr. Mehanathan Muthamilarasan, NIPGR, New Delhi for critically reading the manuscript. We are thankful to NBPGR, Hyderabad and UAS, GKVK, Bangalore, India for providing the seed materials.
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