Abstract
Direct differentiation of shoot buds in Coffea dewevrei was evident from the seedling shoots with collar region and also from collar region end of hypocotyl segments in presence of 40 μM AgNO3, 8.88 μM of BA and 2.85 μM of IAA. Apart from this, shoot end of hypocotyl explants mainly supported yellow friable callus or somatic embryos. Subsequent transfer to the same medium induced secondary somatic embryogenesis. The collar region of the hypocotyl explants not only showed direct organogenesis by producing 1–3 shoots per explant and also able to produce globular somatic embryos and embryogenic yellow friable callus. Similarly direct somatic embryogenesis along with yellow friable embryogenic callus formation on 1/2 strength MS medium comprising 1.47 μM IAA, 2.22 μM BA and 40 μM AgNO3 was noticed from cut portion of in vitro leaf and stalk of regenerated plants. The microshoots rooted well upon subculturing onto the same medium in 6 weeks and showed 60 % survival in green house and resumed growth upon hardening.
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Abbreviations
- AgNO3 :
-
Silver nitrate
- BA:
-
Benzyladenine
- IAA:
-
Indole-3-acetic acid
- MS:
-
Murashige and Skoog
- RH:
-
Relative humidity
References
Ashihara H, Crozier A (2001) Caffeine: a well known but little mentioned compound in plant science. Trends Plant Sci 6:407–41
Beyer EM (1976) A potent inhibitor of ethylene action in plant. Plant Physiol 58:268–271
Carvalho A, Monaco LC (1967) Genetic relationship of selected Coffea species. Ciencia e Cultura 19:151–165
Chevalier A (1947) Les cafeeirs du globe. III. Systematique des cafeires et faux-cefeiers, maladies et insects nuisibles. Paul Lechevalier (ed.), Paris, 356p.
Cho UH, Kasha K (1989) Ethylene production and embryogenesis from anther cultures of barley (Hordeum vulgare). Plant Cell Rep 8:415–417
Cho UH, Kasha K (1992) Relationship of senescence to androgenesis in blarley (Hordeum vulgare L. cv. Klages). J of Plant Physiol 139:299–302
Duncan DR, Williams ME, Zehr B, Widholm JM (1985) The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes. Planta 165:322–332
Ganesh SD, Sreenath HL (1996) Silver nitrate enhanced shoot development in cultured apical shoot buds of Coffea arabica Cv. Cauvery (S.4347). J of Pl Crops 24:577–580
Giridhar P, Indu EP, Vijaya Ramu D, Ravishankar GA (2003) Effect of silver nitrate on in vitro shoot growth of Coffee. Tropical Sci 43:144–146
Giridhar P, Indu EP, Vinod K, Chandrashekar A, Ravishankar GA (2004) Direct somatic embryogenesis from Coffea arabica L and Coffea canephora P ex Fr. under the influence of ethylene action inhibitor- silver nitrate. Acta Physiol Planta 26:299–305
Giridhar P, Obul Reddy B, Ravishankar GA (2001) Silver nitrate influences in vitro shoot multiplication and root formation in Vanilla planifolia Andr. Curr Sci 81:1166–1170
Kong L, Yeung C (1994) Effects of ethylene and ethylene inhibitors on white spruce somatic embryo maturation. Plant Sci 104:71–80
Lopes CR, Monaco LC (1979) Chemotaxonomic studies of some species of the genus Coffea. J of Plant Crops 7:6–14
Mazzafera P, Crozier A, Magalhães AC (1991) Caffeine metabolism in Coffea arabica and other species of coffee. Phytochem 30:13913–3916
Mazzafera P (1993) 7-methyl xanthine is not involved in caffeine catabolism in Coffea dewevrei. J Agric Food Chem 41:1541–1543
Murashige M, Skoog TA (1962) Revised medium for rapid growth and bioassay of tobacco tissue cultures. Physiol Plantarum 15:473–497
Pestana MC, Lacorte C, De Freitas VG, Oliveira DE, Mansur E (1999) In vitro regeneration of peanut (Arachis hypogaea L) through organogenesis: Effect of culture temperature and silver nitrate. In vitro Cell and Dev Biol -Plant 35:214–216
Reddy BO, Giridhar P, Ravishankar GA (2001) In vitro rooting of Decalepis hamiltonii Wight and Arn., an endangered shrub by auxins and root promoting agents. Curr Sci 81:1479–1481
Singh SK, Meghwal PR, Sharma HC, Singh SP (2002) Direct shoot organogenesis on hypocotyl explants from in vitro germinated seedlings of Psidium guajava L. cv. Allahabad Safeda. Sci Horticult 95:213–221
Sonia PK, Gulati A, Dahiya S (1998) Direct Organogenesis in Hypocotyl Cultures of Tamarindus indica. Biol Planta 41:331–337
Sridevi V, Giridhar P, Simmi PS, Ravishankar GA (2010) Direct shoot organogenesis on hypocotyl explants with collar region from in vitro seedlings of Coffea canephora Pierre ex. Frohner cv. CxR and Agrobacterium tumefaciens mediated transformation. Pl Cell Tiss and Org Cult 101:339–347
Vinod K, Giridhar P, Ravishankar GA (2009) AgNO3 - a potential regulator of ethylene activity and plant growth modulator. Electronic J of Biotechnol 12:2
Vinod K, Madhava Naidu M, Ravishankar GA (2006a) Developments in Coffee biotechnology- in vitro plant propagation and crop improvement. Pl Cell Tiss Organ Cult 87:49–65
Vinod K, Satyanarayana KV, Sarala Itty S, Indu EP, Giridhar P, Chandrasekar A, Ravishankar GA (2006b) Stable transformation and direct regeneration in Coffea canephora P ex. Fr. by Agrobacterium rhizogenes mediated transformation without hairy-root phenotype. Plant Cell Rep 25:214–222
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Authors are grateful to Department of Science and Technology, Government of India, New Delhi for financial support.
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Sridevi, V., Giridhar, P. In vitro shoot growth, direct organogenesis and somatic embryogenesis promoted by silver nitrate in Coffea dewevrei . J. Plant Biochem. Biotechnol. 23, 112–118 (2014). https://doi.org/10.1007/s13562-012-0186-2
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DOI: https://doi.org/10.1007/s13562-012-0186-2