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Isolation and characterization of an AP2/ERF-type drought stress inducible transcription factor encoding gene from rice

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Abstract

A genomic DNA fragment of 964 bp corresponding to AP2/ERF family transcription factor was isolated from drought tolerant Oryza sativa L. cv. ‘N-22’ (AP2/ERF-‘N-22’) and Oryza sativa L. cv. ‘Teipei 309’ (AP2/ERF-jap). The cDNA sequence of AP2/ERF-‘N-22’ (732 bp) was amplified using gene specific primers. The gene contains two exons and a single intron. The deduced protein of the AP2/ERF-‘N-22’ contains a potential nuclear localization signal, a possible regulation domain and an AP2 DNA binding domain of 60 amino acids. Northern and RT-PCR analysis showed that transcript of AP2/ERF-‘N-22’ accumulates in response to WDS and the Southern analysis indicated the presence of single copy of AP2/ERF-‘N-22’ gene in Oryza sativa genome. Phylogenetic analysis of AP2/ERF family revealed that AP2/ERF-‘N-22’ belongs to group Va along with SHN clade of AP2/ERF protein which activates wax biosynthesis. A higher epicuticular wax content was observed at decreased RWC and positively correlated with AP2/ERF-‘N-22’ expression.

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Abbreviations

AP2:

Apetala

WDS:

Water deficit stress

ERF:

Ethylene responsive factor

DRE/CRT:

Drought responsive element/C-repeat element

EREBP:

Ethylene responsive element binding protein

ABA:

Abscisic acid

RT-PCR:

Reverse transcriptase PCR

EREB:

Ethylene responsive element binding protein

AP:

APETALA

ABA:

Abscisic acid

DREB:

Drought responsive element binding protein

RWC:

Relative water content

SHN/WIN:

Shine/wax inducer

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Author notes

  1. Sangita Yadav

    Present address: Division of Biochemistry, NBPGR, New Delhi, 110012, India

Authors and Affiliations

  1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012, India

    Ibandalin Mawlong, Kishwar Ali, Devika Kurup & Aruna Tyagi

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  1. Ibandalin Mawlong
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  2. Kishwar Ali
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  3. Devika Kurup
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  4. Sangita Yadav
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Correspondence to Aruna Tyagi.

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Mawlong, I., Ali, K., Kurup, D. et al. Isolation and characterization of an AP2/ERF-type drought stress inducible transcription factor encoding gene from rice. J. Plant Biochem. Biotechnol. 23, 42–51 (2014). https://doi.org/10.1007/s13562-012-0185-3

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  • DOI: https://doi.org/10.1007/s13562-012-0185-3

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