Journal of Plant Biochemistry and Biotechnology

, Volume 22, Issue 4, pp 434–440 | Cite as

Biosynthesis, characterization and antibacterial activity of silver nanoparticles by aqueous Annona squamosa L. leaf extract at room temperature

  • U. B. Jagtap
  • V. A. Bapat
Original Article


Silver nanoparticles (AgNPs) have gained great interest in nanotechnology, biotechnology and medicine. The green synthesis of nanoparticles has received an increasing attention because of it’s maximize efficiency and minimize health and environmental hazards as compared to other conventional chemical synthesis. In this study, we reported biosynthesis of AgNPs by aqueous Annona squamosa L. leaf extract and its characterization by UV-visible spectroscopy (UV–vis), Field emission gun scanning electron microscopy (FEG-SEM), X-ray energy dispersive spectroscopy (EDX), Transmission electron microscopy (TEM), Selected-area electron diffraction (SAED) and Fourier transform infra-red spectroscopy (FTIR). The results indicated that AgNPs formed were spherical in shape with size ranging from 14 to 40 nm with an average diameter 28.47 nm. Furthermore, it was observed that the AgNPs exhibited an antibacterial activity against different Gram positive and Gram negative microorganisms. Our report confirmed that the ALE is a very good eco-friendly and nontoxic bioreductant for the synthesis of AgNPs and opens up further opportunities for fabrication of antibacterial drugs, medical devices and wound dressings.


Annona squamosa L. Antibacterial activity Green synthesis Nanomedicine Silver nanoparticles 



Silver Nitrate


Silver Nanoparticles


Annona squamosa L. Leaf Extract


X-ray Energy Dispersive Spectroscopy


Fourier Transform Infrared Spectroscopy




Selected Area Electron Diffraction


Field Emission Gun Scanning Electron Microscopy


Transmission Electron Microscopy


UV-Visible Spectroscopy



UBJ thanks Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing SRF and VAB thanks Indian National Science Academy, New Delhi, India, for Senior Scientist Fellowship. We acknowledge the staffs SAIF, IIT-Mumbai, for FEG-SEM and TEM analysis.


  1. Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A (2010) Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B 81:81–86CrossRefGoogle Scholar
  2. Babu SA, Prabu HG (2011) Synthesis of AgNPs using the extract of Calotropis procera flower at room temperature. Mater Lett 65:1675–1677CrossRefGoogle Scholar
  3. Bakar NHHA, Ismail J, Bakar MA (2007) Synthesis and characterization of silver nanoparticles in natural rubber. Mater Chem Phys 104:276CrossRefGoogle Scholar
  4. Bankar A, Joshi B, Kumara AR, Zinjarde S (2010) Banana peel extract mediated novel route for the synthesis of silver nanoparticles. Colloids Surf A 368:58–63CrossRefGoogle Scholar
  5. Bar H, Bhui DK, Sahoo GP, Sarkar P, De SP, Misra A (2009a) Green synthesis of silver nanoparticles using latex of Jatropha curcas. Colloids Surf A 339:134–139CrossRefGoogle Scholar
  6. Bar H, Bhui DK, Sahoo GP, Sarkar P, Pyne S, Misra A (2009b) Green synthesis of silver nanoparticles using seed extract of Jatropha curcas. Colloids Surf A 348:212–216CrossRefGoogle Scholar
  7. Chaloupka K, Malam Y, Seifalian AM (2010) Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol 28:580–588PubMedCrossRefGoogle Scholar
  8. Cos P, Vlietinck AJ, Berghe DV, Maes L (2006) Anti-infective potential of natural products: how to develop a stronger in vitro ‘proof-of-Concept’. J Ethnopharmacol 106:290–302PubMedCrossRefGoogle Scholar
  9. Croes S, Stobberingh EE, Stevens KNJ, Knetsch MLW, Koole LH (2011) Antimicrobial and anti-thrombogenic features combined in hydrophilic surface coatings for skin-penetrating catheters. Synergy of co-embedded silver particles and heparin. ACS Appl Mater Interfaces 3:2543–2550PubMedCrossRefGoogle Scholar
  10. Dankovich TA, Gray DG (2011) Bactericidal paper impregnated with silver nanoparticles for point-of-use water treatment. Environ Sci Technol 45:1992–1998PubMedCrossRefGoogle Scholar
  11. Eby DM, Luckarift HR, Johnson GR (2009) Hybrid antimicrobial enzyme and silver nanoparticle coatings for medical instruments. ACS Appl Mater Interfaces 1:1553–1560PubMedCrossRefGoogle Scholar
  12. Elumalai EK, Prasad TNVKV, Hemachandran J, Therasa VS, Thirumalai T, David E (2010) Extracellular synthesis of silver nanoparticles using leaves of Euphorbia hirta and their antibacterial activities. J Pharmaceutical Sci Res 2:549–554Google Scholar
  13. Fayaz AM, Balaji K, Girilal M, Kalaichelvan PT, Venkatesan R (2009) Mycobased synthesis of silver nanoparticles and their incorporation into sodium alginate films for vegetable and fruit preservation. J Agric Food Chem 57:6246–6252CrossRefGoogle Scholar
  14. Huang Y, Li X, Liao Z, Zhang G, Liu Q, Tang J, Peng Y, Liu X, Luo Q (2007) A randomized comparative trial between acticoat and SD-Ag in the treatment of residual burn wounds, including safety analysis. Burns 33:161–166PubMedCrossRefGoogle Scholar
  15. Huang J, Zhan G, Zheng B, Sun D, Lu F, Lin Y, Chen H, Zheng Z, Zheng Y, Li Q (2011) Biogenic silver nanoparticles by Cacumen platycladi extract: synthesis, formation mechanism, and antibacterial activity. Ind Eng Chem Res 50:9095–9106CrossRefGoogle Scholar
  16. Jiranek WA, Hanssen AD, Greenwald AS (2006) Antibiotic-loaded bone cement for infection prophylaxis in total joint replacement. J Bone Joint Surg 88:2487–2500PubMedCrossRefGoogle Scholar
  17. Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K (2011) Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity. Spectrochimica Acta A 79:594–598CrossRefGoogle Scholar
  18. Kelly FM, Johnston JH (2011) Colored and functional silver nanoparticle wool fiber composites. ACS Appl Mater Interfaces 3:1083–1092PubMedCrossRefGoogle Scholar
  19. Kora AJ, Sashidhar RB, Arunachalam J (2010) Gum kondagogu (Cochlospermum gossypium): a template for the green synthesis and stabilization of silver nanoparticles with antibacterial application. Carbohydr Poly 82:670–679CrossRefGoogle Scholar
  20. Kumar A, Vemula PK, Ajayan PM, John G (2008) Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil. Nature Mater 7:236–241CrossRefGoogle Scholar
  21. Kumar V, Yadav SC, Yadav SK (2010) Syzygium cumini leaf and seed extract mediated biosynthesis of silver nanoparticles and their characterization. J Chem Technol Biotechnol 85:1301–1309CrossRefGoogle Scholar
  22. Lara HH, Ayala-Nuñez NV, Ixtepan-Turrent L, Rodriguez-Padilla C (2010) Mode of antiviral action of silver nanoparticles against HIV-1. J Nanobiotechnology 8:1PubMedCrossRefGoogle Scholar
  23. Li Y, Leung P, Song QW, Newton E (2006) Antimicrobial effects of surgical masks coated with nanoparticles. J Hospital Infection 62:58–63CrossRefGoogle Scholar
  24. Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L, Zhang Q (2007) Green synthesis of silver nanoparticles using Capsicum annuum L. extract. Green Chem 9:852–858CrossRefGoogle Scholar
  25. Liaw C-C, Wu T-Y, Chang F-R, Wu Y-C (2010) Historic perspectives on annonaceous acetogenins from the chemical bench to preclinical trials. Planta Med 76:1390–1404PubMedCrossRefGoogle Scholar
  26. Lukman AI, Gong B, Marjo CE, Roessner U, Harris AT (2011) Facile synthesis, stabilization, and anti-bacterial performance of discrete Ag nanoparticles using Medicago sativa seed exudates. J Colloid Interface Sci 353:433–444PubMedCrossRefGoogle Scholar
  27. Mock JJ, Barbic M, Smith DR, Shultz DA, Shultz S (2002) Shape effect in plasmon resonance of individual colloidal silver nanoparticles. J Chem Phys 116:6755–6759CrossRefGoogle Scholar
  28. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346–2353PubMedCrossRefGoogle Scholar
  29. MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M (2011) Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B 85:360–365CrossRefGoogle Scholar
  30. Mulvihill MJ, Beach ES, Zimmerman JB, Anastas PT (2011) Green chemistry and green engineering: a framework for sustainable technology development. Annu Rev Environ Resour 36:271–293CrossRefGoogle Scholar
  31. Nabikhan A, Kandasamy K, Raj A, Alikunhi NM (2010) Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L. Colloids Surf B 79:488–493CrossRefGoogle Scholar
  32. Narayanan KB, Sakthivel N (2011) Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Adv Colloid Interface 69:59–79CrossRefGoogle Scholar
  33. Njagi EC, Huang H, Stafford L, Genuino H, Galindo HM, Collins JB, Hoag GE, Suib SL (2011) Biosynthesis of iron and silver nanoparticles at room temperature using aqueous Sorghum bran extracts. Langmuir 27:264–271PubMedCrossRefGoogle Scholar
  34. Philip D (2010) Green synthesis of gold and silver nanoparticles using Hibiscus rosasinensis. Physica E 42:1417–1424CrossRefGoogle Scholar
  35. Prasad TNVKV, Elumalai EK, Savithramma N (2010) Production of biogenic silver nanoparticles using Boswellia ovalifoliolata stem bark. Digest J Nanomater Biostruct 5:369–372Google Scholar
  36. Prathna TC, Chandrasekaran N, Raichur AM, Mukherjee A (2011) Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids Surf B 82:152–159CrossRefGoogle Scholar
  37. Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83PubMedCrossRefGoogle Scholar
  38. Salunkhe RB, Patil SV, Patil CD, Salunke B (2011) Larvicidal potential of silver nanoparticles synthesized using fungus Cochliobolus lunatus against Aedes aegypti (Linnaeus, 1762) and Anopheles stephensi Liston (Diptera; Culicidae). Parasitol Res 109:823–831PubMedCrossRefGoogle Scholar
  39. Sanpui P, Murugadoss A, Durga Prasad PV, Ghosh SS, Chattopadhyay A (2008) The antibacterial properties of a novel chitosan-Ag-nanoparticle composite. Inter J Food Microbiol 124:142–146CrossRefGoogle Scholar
  40. Sastry M, Mayyaa KS, Bandyopadhyay K (1997) pH dependent changes in the optical properties of carboxylic acid derivatized silver colloid particles. Colloids Surf A 127:221–222CrossRefGoogle Scholar
  41. Sathishkumar M, Sneha K, Yun YS (2010) Immobilization of silver nanoparticles synthesized using Curcuma longa tuber powder and extract on cotton cloth for bactericidal activity. Bioresour Technol 101:7958–7965PubMedCrossRefGoogle Scholar
  42. Saxena A, Tripathi RM, Singh RP (2010) Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Digest J Nanomater Biostruct 5:427–432Google Scholar
  43. Shankar SS, Ahmad A, Sastry M (2003) Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol Progr 19:1627–1631CrossRefGoogle Scholar
  44. Shankar SS, Rai A, Ahmad A, Sastry M (2004) Rapid synthesis of Au, Ag, and bimetallic Au Core–Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 275:496–502PubMedCrossRefGoogle Scholar
  45. Shrivastava S, Bera T, Singh SK, Singh G, Ramachandrarao P, Dash D (2009) Characterization of antiplatelet properties of silver nanoparticles. ACS Nano 3:1357–1364PubMedCrossRefGoogle Scholar
  46. Vigneshwaran N, Ashtaputre NM, Varadarajan PV, Nachane RP, Paralikar KM, Balasubramanya RH (2007) Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. Mater Lett 61:1413–1418CrossRefGoogle Scholar
  47. Vilchis-Nestor AR, Sanchez-Mendieta V, Camacho-Lopez MA, Gomez-Espinosa RM, Camacho-Lopez MA, Arenas-Alatorre JA (2008) Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Mater Lett 62:3103–3105CrossRefGoogle Scholar
  48. Virkutyte J, Varma RS (2011) Green synthesis of metal nanoparticles: Biodegradable polymers and enzymes in stabilization and surface functionalization. Chem Sci 2:837CrossRefGoogle Scholar
  49. Vlachou E, Chipp E, Shale E, Wilson YT, Papini R, Moiemen NS (2007) The safety of nanocrystalline silver dressings on burns: a study of systemic silver absorption. Burns 33:979–985PubMedCrossRefGoogle Scholar
  50. Xing ZC, Chae WP, Baek JY, Choi MJ, Jung Y, Kang IK (2010) In vitro assessment of antibacterial activity and cytocompatibility of silver-containing PHBV nanofibrous scaffolds for tissue engineering. Biomacromolecules 11:1248–1253PubMedCrossRefGoogle Scholar
  51. Yang JY, Huang CY, Chuang SS, Chen CC (2007) A clinical experience of treating exfoliative wounds using nanocrystalline silver-containing dressings (acticoat1). Burns 33:93–797CrossRefGoogle Scholar

Copyright information

© Society for Plant Biochemistry and Biotechnology 2012

Authors and Affiliations

  1. 1.Department of BiotechnologyShivaji UniversityKolhapurIndia

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