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Journal of Plant Biochemistry and Biotechnology

, Volume 22, Issue 4, pp 409–413 | Cite as

Total phenolics, flavonoids and antioxidant activity of Saptarangi (Salacia chinensis L.) fruit pulp

  • J. J. Chavan
  • U. B. Jagtap
  • N. B. Gaikwad
  • G. B. Dixit
  • V. A. Bapat
Original Article

Abstract

Salacia chinensis L. has various beneficial properties including antidiabetic and antioxidant activity. The S. chinensis fruit pulp (SCFP) was extracted with four different solvents (Methanol, ethanol, acetone and water) and was screened for total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (AOA). The AOA was assessed by evaluating the 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and metal chelating assay. Methanolic SCFP extract exhibited the highest phenolic content (3.20 ± 0.12 mg GAE/g FW) whereas, ethanolic extract showed highest flavonoid content (0.31 ± 0.68 mg RE/g FW). The methanolic extract possesses highest antioxidant activity towards DPPH (92.44 %), FRAP (1.939 O.D) and metal chelating activity (74.16 %). AOA (DPPH and FRAP) was significantly correlated with TPC. The results indicated that SCFP is a good natural source of antioxidant compounds for use in food and pharmaceutical industry.

Keywords

DPPH radical FRAP radical Metal chelating Salacia chinensis Total flavonoids Total phenolic content 

Abbreviations

DPPH

1 1-diphenyl-2-picrylhydrazyl

FRAP

Ferric reducing antioxidant power

GAE

Gallic acid equivalent

SCFP

Salacia chinensis fruit pulp

TFC

Total flavonoid content

TPTZ

2 4, 6-tripyridyl-s-triazine

References

  1. Benzie IF, Strain JJ (1996) Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76PubMedCrossRefGoogle Scholar
  2. Chang C, Yang M, Wen H, Chern J (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182Google Scholar
  3. Dinis T, Madeira V, Almeida T (1994) Action of phenolic derivatives (acetaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and peroxyl radical scavengers. Arch Biochem Biophys 315:161–165PubMedCrossRefGoogle Scholar
  4. Govindaraj Y, Melanaphuru V, Agrahari V, Gupta S, Nema RK (2009) Genotoxicity studies of Mangiferin isolated from Salacia chinensis Linn. Acad J Plant Sci 2:199–204Google Scholar
  5. Guha S, Ghosal S, Chattopadhyay U (1996) Antitumor, immunomodulatory and anti-HIV effect of mangiferin, a naturally occurring glucosylxanthone. Chemotherapy 42:443PubMedCrossRefGoogle Scholar
  6. Harris GG, Brannan RG (2009) A preliminary evaluation of antioxidant compounds, reducing potential and radical scavenging of pawpaw (Asimina tribloba) fruit pulp from different stages of ripeness. LWT - Food Sci Technol 42:275–279CrossRefGoogle Scholar
  7. Hossain MA, Rahman SMM (2011) Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Res Int 44:672–676CrossRefGoogle Scholar
  8. Jagtap UB, Panaskar SN, Bapat VA (2010) Evaluation of antioxidant capacity and phenol content in Jackfruit (Artocarpus heterophyllus Lam.) fruit pulp. Plant Foods Hum Nutr 65:99–104PubMedCrossRefGoogle Scholar
  9. Naoki Y, Kengo M, Masaki K, Yasuhiro Y, Toshiya K, Zheng Q, Akira H, Johji Y, Hideki M (2001) The inhibitory effects of mangiferin, a naturally occurring glucosyl-xanthone, in bowel carcinogenesis of male F344 rats. Cancer Lett 163:163CrossRefGoogle Scholar
  10. Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16:144–158Google Scholar
  11. Sofidiya MO, Odukoya OA, Familoni OB, Inya-Agha SI (2006) Free radical scavenging activity of some Nigerian medicinal plant extracts. Pak J Biol Sci 9:1438–1441CrossRefGoogle Scholar
  12. Vaidya ADB, Devasagayam TPA (2007) Current status of herbal drugs in India: an overview. J Clin Biochem Nutr 41:1–11PubMedCrossRefGoogle Scholar
  13. Yoshikawa M, Nishida N, Shimoda H, Takada M, Kawahara Y, Matsuda H (2001) Polyphenol constituents from Salacia species: quantitative analysis of mangiferin with alpha-glucosidase and aldose reductase inhibitory activities. Yaku Zas 121:371–378CrossRefGoogle Scholar
  14. Zhou K, Yu L (2004) Antioxidant properties of bran extracts from Trego wheat grown at different locations. J Agri Food Chem 52:1112–1117CrossRefGoogle Scholar

Copyright information

© Society for Plant Biochemistry and Biotechnology 2012

Authors and Affiliations

  • J. J. Chavan
    • 1
    • 3
  • U. B. Jagtap
    • 1
    • 2
  • N. B. Gaikwad
    • 1
  • G. B. Dixit
    • 1
  • V. A. Bapat
    • 1
    • 2
  1. 1.Department of BotanyShivaji UniversityKolhapurIndia
  2. 2.Department of BiotechnologyShivaji UniversityKolhapurIndia
  3. 3.P.G. Department of BotanyY.C. Institute of ScienceSataraIndia

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