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Jasmonates modulate the promotion effects induced by SNP on root development of wheat under osmotic stress through lipoxygenase activation

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Abstract

We investigated promotion effects of exogenous sodium nitroprusside (SNP) on wheat seedling (Triticum aestivum L.) lateral root (LR) and root hair development, and the relationship between endogenous jasmonate (JA) production and activity changes of lipoxygenase (LOX) isoenzymes under osmotic stress generated by 15 % PEG-6000. Our results showed that 25 or 50 μM SNP could significantly increase LR length and number whether or not the seedlings were under PEG stress. When 50 μM cPTIO, 50 μM SHAM or 50 μM NDGA was supplemented, the promotion effects of SNP were blocked. SNP could also induce the production of endogenous JAs in roots, and 25 μM SNP induced the maximum JA content. The effect of SNP on JA production could also be blocked by adding cPTIO, SHAM or NDGA. Furthermore, the activity of lipoxygenase (LOX) in roots was affected by SNP; the maximal activity of LOX also occurred in the roots treated by 25 μM SNP under PEG stress, or 50 μM SNP without PEG stress. LOX isoenzymes in roots were detected by electrophoresis; the results showed that 25 μM SNP could noticeably increase the activities of LOXII and LOXIII under PEG stress. Our results suggest that, under osmotic stress generated by PEG, the promotion effects of exogenous SNP on wheat LR and root hair development could be mediated by endogenous JAs through LOX activation.

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Abbreviations

LR:

Lateral root

NO:

Nitric oxide

PEG:

Polyethylene glycol

SNP:

Sodium nitroprusside

cPTIO:

2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

JAs:

Jasmonates

SHAM:

Salicylhydroxamic acid

NDGA:

Nordihydroguaiaretic acid

LOX:

Lipoxygenase

DMSO:

Dimethylsulfoxide

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Acknowledgements

This research is supported by National Natural Science Foundation of China (30871461 and 31000363). We are very grateful to Prof. Kai Xia for his assistance in the measuring of endogenous JAs, and Prof. Qingya Wang for his taking pictures of wheat roots.

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, People’s Republic of China

    Xianye Wang, Ning Wang, Qi Rui, Peng Zhang & Langlai Xu

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  1. Xianye Wang
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  2. Ning Wang
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  3. Qi Rui
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  4. Peng Zhang
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  5. Langlai Xu
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Correspondence to Langlai Xu.

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Xianye Wang and Ning Wang contributed equally to this work

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Wang, X., Wang, N., Rui, Q. et al. Jasmonates modulate the promotion effects induced by SNP on root development of wheat under osmotic stress through lipoxygenase activation. J. Plant Biochem. Biotechnol. 22, 295–303 (2013). https://doi.org/10.1007/s13562-012-0158-6

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  • DOI: https://doi.org/10.1007/s13562-012-0158-6

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