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Studies on the in vitro regenerative competence of aerial roots of two horticultural important Cymbidium species

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Abstract

In the present study aerial roots were successfully used as explants source for in vitro propagation of Cymbidium aloifolium and Cymbidium iridioides. Aerial roots of ~5–6 week old from axenic cultures were cultured on MS medium adjuncts with different additives. In C. aloifolium within 20 days of culture ~60% of explants responded positively on MS medium containing sucrose (3%, w/v) and Kn (3 μM) and formed PLBs. While in C. iridioides ~50% root explants responded positively on medium enriched with sucrose (3%), AC (0.1%) and IAA (3 μM) after 40 days of culture. The shoot buds/PLBs converted into plantlets when maintained on regeneration medium. Of the three basal media tested, MS medium supported optimum regeneration and culture proliferation in both the species. In C. aloifolium ~12 shoot buds developed on medium nourished with sucrose (3%) and BA (3 μM) but in C. iridioides optimum regeneration was achieved when medium supplemented with sucrose (3%), CW (15%), CH (100 mg L−1) and ~20 shoot buds formed per subculture. The well rooted plantlets were acclimatized for 3–4 week in 1/10th MS salt solution containing sucrose (1%), charcoal pieces, brick pieces and chopped mosses as support under normal laboratory conditions. The hardened plants were transferred to potting mix where 80 and 75% of transplants survived in C. aloifolium and C. iridioides respectively after 2 months of transfer.

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Abbreviations

BA:

Benzyl adenine

2,4-D:

2,4 dichlorophenoxy acetic acid

IAA:

Indole 3 acetic acid

Kn:

Kinetin

NAA:

α-naphthalene acetic acid

PLBs:

Protocorm-like bodies

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Acknowledgement

Authors are thankful to the Vice Chancellor, Nagaland University for the basic facilities for the present study and UGC fellowship to Miss. Aolemla Pongener.

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Authors and Affiliations

  1. Department of Botany, Nagaland University, Headquarters: Lumami, Lumami, 798627, Nagaland, India

    Chitta Ranjan Deb & Aolemla Pongener

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  1. Chitta Ranjan Deb
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  2. Aolemla Pongener
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Correspondence to Chitta Ranjan Deb.

Electronic supplementary materials

Below is the link to the electronic supplementary material.

Supplementary Table 1

Effects of PGRs on culture initiation from aerial roots (5–6 wk old) of C. iridioides from in vitro source (DOC 24 kb)

Supplementary Table 2

Effects of different PGRs on plant regeneration and mass multiplication of C. aloifolium* and C. iridioides** (DOC 25 kb)

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Deb, C.R., Pongener, A. Studies on the in vitro regenerative competence of aerial roots of two horticultural important Cymbidium species. J. Plant Biochem. Biotechnol. 21, 235–241 (2012). https://doi.org/10.1007/s13562-011-0099-5

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