Skip to main content
SpringerLink
Log in

Cold-induced dehydrins from Poncirus trifoliata localized in the nucleus

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Cold regulated dehydrins, COR11, COR19 and the C-terminal part of the COR11 genes from Poncirus trifoliata were amplified and cloned into the pCAMBIA1301 binary vector as fusion with beta-glucuronidase (GUS) reporter gene to determine functionality of the bipartite nuclear localization signal (NLS). When these plasmids and the control plasmids were bombarded into the onion epidermal cells, the expression of the GUS was located in the nucleus and that the COR genes are able to localize GUS in the nucleus indicating that the previously proposed NLS of these genes are functional. Deletion studies showed that the C-terminal half of the COR11 is sufficient for the nuclear localization of the fusion GUS reporter gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Asghar R, Fenton RD, DeMason DA, Close TJ (1994) Nuclear and cytoplasmic localization of maize embryo and akurone dehydrin. Protoplasma 177:87–94

    Article  CAS  Google Scholar 

  • Baker J, Steele C, Dure L (1988) Sequence and characterization of six lea proteins and their genes from cotton. Plant Mol Biol 11:277–291

    Article  CAS  Google Scholar 

  • Borovskii GB, Stupnikova IV, Antipina AI, Downs CA, Voinikov VK (2000) Accumulation of dehydrin-like-proteins in the mitochondria of cold-treated plants. J Plant Physiol: 797–800

  • Borovskii GB, Stupnikova IV, Antipina AI, Voinikov VK (2002) Accumulation of dehydrins and ABA-inducible proteins in wheat seedlings during low-temperature acclimation. Russian J Plant Physiol: 49

  • Cai QY, Moore GA, Guy CL (1995) An unusual group-2 LEA gene family in citrus responsive to low-temperature. Plant Mol Biol 29:11–23

    Article  PubMed  CAS  Google Scholar 

  • Campbell SA, Close TJ (1997) Dehydrins: genes, proteins, and associations with phenotypic traits. New Phytologist 137:61–74

    Article  CAS  Google Scholar 

  • Choi DW, Zhu B, Close TJ (1999) The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo. Theor Appl Genet 98:1234–1247

    Article  CAS  Google Scholar 

  • Close TJ (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant 97:795–803

    Article  CAS  Google Scholar 

  • Close TJ (1997) Dehydrins: a commonality in the response of plants to dehydration and low temperature. Physiol Plant 100:291–296

    Article  CAS  Google Scholar 

  • Danyluk J, Perron A, Houde M, Limin A, Fowler B, Benhamou N, Sarhan F (1998) Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat. Plant Cell 10:623–638

    Article  PubMed  CAS  Google Scholar 

  • Goday JA, Jensen AB, Culianezmacia FA, Alba MM, Figueras M, Serratosa J, Torrent M, Pages M (1994a) The maize abscisic acid responsive protein Rab17 is located in the nucleus and interacts with nuclear localization signals. Plant Cell 6:351–360

    Article  PubMed  CAS  Google Scholar 

  • Goday JA, Lunar R, Torres-Schumann S, Moreno RRJ, Pintor-Toro JA (1994b) Expression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants. Plant Mol Biol 26:1921–1934

    Article  Google Scholar 

  • Hara M, Terashima S, Fukaya T, Kuboi T (2003) Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco. Planta 217:290–298

    PubMed  CAS  Google Scholar 

  • Houde M, Daniel C, Lachapelle M, Allard F, Laliberte S, Sarhan F (1995) Immunolocalization of freezing tolerance associated proteins in the cytoplasm and nucleoplasm of wheat crown tissues. Plant J 8:583–593

    Article  PubMed  CAS  Google Scholar 

  • Ismail AM, Hall AE, Close TJ (1999) Purification and partial characterization of a dehydrin involved in chilling tolerance during seedling emergence of cowpea. Plant Physiol 120:237–244

    Article  PubMed  CAS  Google Scholar 

  • McLean BG, Eubanks S, Meagher RB (1990) Tissue-specific expression of divergent actins in soybean root. Plant Cell 2:335–344

    Article  PubMed  CAS  Google Scholar 

  • Monroy AF, Castonguay Y, Laberge S, Sarhan FL, Vezina PR, Dhindsa S (1993) A new cold-induced alfalfa gene is associated with enhanced hardening at subzero temperature. Plant Physiol: 873–879

  • Mtwisha L, Brandt W, McCready S, Lindsey GG (1998) HSP 12 is a LEA-like protein in Saccharomyces cerevisiae. Plant Mol Biol 37:513–521

    Article  PubMed  CAS  Google Scholar 

  • Mundy JN, Chua H (1988) Abscisic-acid and water stress induce the expression of a novel rice gene. EMBO J 7:2279–2286

    PubMed  CAS  Google Scholar 

  • Neven LG, Haskell DW, Hofig A, Li QB, Guy CL (1993) Characterization of a spinach gene responsive to low temperature and water stress. Plant Mol Biol 21:291–305

    Article  PubMed  CAS  Google Scholar 

  • Rinne PLH, Kaikuranta PLM, van der Plas CL, van der Schoot HW (1999) Dehydrins in cold-acclimated apices of birch (Betula pubescens Ehrh.): production, localization and potential role in rescuing enzyme function during dehydration. Planta 209:377–388

    Article  PubMed  CAS  Google Scholar 

  • Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol Biol 50:571–599

    Article  PubMed  CAS  Google Scholar 

  • Wisniewski M, Balsamo R, Close TJ, Artlip T, Arora R, Yu XM, Griffith M (1999) Purification, immunolocalization, cryoprotective, and antifreeze activity of PCA60: a dehydrin from peach (Prunus persica). 105:600–608

Download references

Author information

Authors and Affiliations

  1. Department of Agricultural Biotechnology, Faculty of Agriculture, Suleyman Demirel University, Isparta, Turkey, 32260

    Mehtap Şahin-Çevik

  2. Department of Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32611, USA

    Gloria A. Moore

Authors
  1. Mehtap Şahin-Çevik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gloria A. Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mehtap Şahin-Çevik.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Şahin-Çevik, M., Moore, G.A. Cold-induced dehydrins from Poncirus trifoliata localized in the nucleus. J. Plant Biochem. Biotechnol. 21, 134–139 (2012). https://doi.org/10.1007/s13562-011-0074-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13562-011-0074-1

Keywords

Search

Navigation