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Regeneration of pineapple (Ananas comosus L.) plant through somatic embryogenesis

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Abstract

An efficient protocol for a complete plant regeneration by somatic embryogenesis was developed with Smooth Cayenne pineapple (Ananas comosus L.). Previous works showed that this species is responsive to somatic embryogenesis. In the present work the influence of components of culture medium in the induction, development and conversion of somatic embryos was investigate in order to establish a somatic embryogenesis protocol. Nodular callus (83.67%) was initiated from leaf explants of young plants on CIM3 medium. The highest frequency (37.6%) of embryogenic callus induction was obtained from 4-week-old calluses on EIM3 medium supplemented with 3.0 mg/l picloram. The highly organized callus induction and the development of somatic embryos were achieved after the transfer of callus clumps onto EIM3 medium containing 1.0 mg/l BAP + 0.1 mg/l NAA. The frequency of somatic embryo formation was of 39.5 ± 2.45 embryos per callus. Up to 97% of the somatic embryos were converted into complete plants within 4 week on MSB medium with 1.0 mg/l BAP + 0.05 mg/l GA3 + 500 mg/l glutamine. The continuation of the elongation of the shoots occurred on this medium). Shoots obtained from all the above methods were rooted in MSB medium with activated charcoal. Complete plantlets were transferred onto specially made polyethylene bags containing soil mixture and transferred to the greenhouse. Survival rate of the plantlets under ex vitro conditions was 98% and maximum average number of plantlets (80 ± 0.6). The well-developed plantlets were transferred to an open field where the plants produced normal fruits.

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Abbreviations

BAP:

6-benzylaminopurine

GA3 :

Gibberellic acid

IBA:

Indole-3-butyric acid

KIN:

Kinetin

MS:

Murashige and Skoog

NAA:

Naphthalene acetic acid

picloram:

4-amino-3,5,6-trichloropicolinic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

References

  • Arnold SV, Sabala I, Bozhkov P, Dyachok J, Filonova L (2002) Developmental pathways of somatic embryogenesis. Plant Cell Tiss Organ Cult 69:233–249

    Article  Google Scholar 

  • Chakroun A, Jemmali A, Ben Hamed K, Abdelli C, Druart P (2007) Effet du nitrate d’ammonium sur le développement et l’activité des enzymes anti-oxydantes du fraisier (Fragaria x ananassa L.) micropropagé. Biotechnol Agron Soc Environ 11(2):89–95

    CAS  Google Scholar 

  • Dal Vesco LL, Guerra MP (2001) The effectiveness of nitrogen sources in Feijoa somatic embryogenesis. Plant Cell Tiss Organ Cult 64:19–25

    Article  CAS  Google Scholar 

  • Daquinta MA, Cisneros A, Rodriguez Y, Escalona M, Perez MC, Luna I, Borroto CG (1997) Somatic embryogenesis in pineapple (Ananas comosus L.) Merr.) In: Martin-Prevel P, Hugon R (eds) Proceeding of the Second International Pineapple Symposium. Acta Hort 425:251–257

  • Devi YS, Mujib A, Kundu SC (1997) Efficient regenerative potential from long term culture of pineapple. Phytomorphology 47:255–259

    Google Scholar 

  • Dublin P, Enjalric F, Lardet L, Carron MP, Trolinder N, Pannetier C (1991) Estate crops. In: Debergh PC, Zimmerman RH (eds) Micropropagation technology and application. Kluwer, Dordrecht, pp 337–361

    Google Scholar 

  • Filner P (1966) Regulation of nitrate reductase in cultured tobacco cells. Biochem Biophys Acta 118:299–310

    PubMed  CAS  Google Scholar 

  • Firoozabady E, Moy Y (2004) Regeneration of pineapple plants via somatic embryogenesis and organogenesis. In Vitro Cell Dev Biol Plant 40:67–74. doi:10.1079/IVP2003494

    Google Scholar 

  • Firoozabady E, Heckert M, Gutterson N (2006) Transformation and regeneration of pineapple. Plant Cell Tiss Organ Cult 84:1–16. doi:10.1007/s11240-005-1371-y

    Article  Google Scholar 

  • Fuentes-Cerda CFJ, Monforte-Gonzalez M, Mendez-Zeel M, Rojas-Herrera R, Loyola-Vargas VM (2006) Modification of the embryogenic response of Coffea arabica by the nitrogen source. Biotechnol Lett 23:1341–1343

    Article  Google Scholar 

  • Fukunaga Y, King J (1982) The effect of L-amino acid on the growth and nitrate reductase activity in cultured cells of Datura innoxia. Plant Sci Lett 24:45–54

    Article  CAS  Google Scholar 

  • Gangopadhyay G, Roy SK, Basu GS, Mukherjee KK (2009) Agrobacterium-mediated genetic transformation of pineapple var. Queen using a novel encapsulation-based antibiotic selection technique. Plant Cell Tiss Organ Cult 97:295–302

    Article  CAS  Google Scholar 

  • George EF (1993) Plant Propagation by tissue culture: Part I: the technology. Exegetics Ltd, Edington, pp 273–291

    Google Scholar 

  • Gona KK, Omid K (2008) Picloram-induced somatic embryogenesis in leaves of strawberry (Fragaria ananassa L.). Acta Biol Cracov Bot 50:69–72

    Google Scholar 

  • Gonzalez-Arnao MT, Panta A, Roca WM, Escobar RH, Engelmann F (2008) Development and large-scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops. Plant Cell Tiss Organ Cult 92:1–13

    Article  Google Scholar 

  • Greer MS, Kovalchuk I, Eud F (2009) Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestiva. New Biotechnol 26:44–52

    Article  CAS  Google Scholar 

  • Ikram UH (2005) Callus proliferation and somatic embryogenesis in cotton (Gossypium hirsutum L.). Afr J Biotechnol 4:206–209

    Google Scholar 

  • Ikram UH, Yusuf Z (2004) Effect of nitrates on embryo induction efficiency in cotton (Gossypium hirsutum L.). Afr J Biotechnol 6:319–323

    Google Scholar 

  • Jain A, Rout GR, Raina SN (2002) Somatic embryogenesis and plant regeneration from callus cultures of Phlox paniculta Linn. Sci Hortic 94:137–143

    Article  CAS  Google Scholar 

  • Le Van TH, Takamura T, Tanaka M (2004) Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid. Plant Sci 166:1443–1449. doi:10.1016/j.plantsci.2004.01.023

    Article  Google Scholar 

  • Manoj KR, Akhtar N, Jaiswal VS (2007) Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local. Sci Hortic 113:129–133. doi:10.1016/j.scienta.2007.02.010

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) Revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nomura K, Komamime A (1995) Physiological and biochemical aspect of somatic embryogenesis. Curr Plant Sci Biotechnol Agric 20:249–265

    CAS  Google Scholar 

  • Odutayo O, Akinrimisi FB, Ogunbosoye I, Oso RT (2005) Multiple shoot induction from embryo derived callus cultures of cowpea (Vigna unguiculata L.) Walp. Afr J Biotechnol 4(11):1214–1216

    CAS  Google Scholar 

  • Rahman KW, Ahmed MB, Rahman MM, Amin MN, Hossain MS, Ahmed R (2005) Large scale plant regeneration in vitro from leaf derived callus cultures of pineapple [Ananas comosus (L.) Merr. cv. Giant Kew]. Int J Bot 1(2):128–132

    Article  Google Scholar 

  • Samson NP, Campa C, Le Gal L, Noirot M, Thomas G, Lokeswari TS, de Kochko A (2006) Effect of primary culture medium composition on high frequency somatic embryogenesis in different Coffea species. Plant Cell Tiss Organ Cult 86:37–45

    Article  Google Scholar 

  • Selvaraj N, Vasudevan A, Manickavasagam M, Ganapathi A (2006) in vitro organogenesis and plant formation in cucumber. Biol Plant 50(1):123–126

    Article  CAS  Google Scholar 

  • Smith MK, Ko HL, Hamill SD, Sanewski GM (2002) Pineapple Transformation: managing somaclonal variation. Acta Hortic 575:69–74

    Google Scholar 

  • Sripaoraya S, Marchant R, Brian PJ, Davey MR (2003) Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.). In Vitro Cell Dev Biol Plant 39:450–454. doi:10.1079/IVP2003445

    Article  Google Scholar 

  • Stamp JA, Henshaw GG (1987) Somatic embryogenesis from clonal leaf tissues of cassava. Ann Bot 59:445–450

    CAS  Google Scholar 

  • Thiruvengadam M, Mohamed SV, Yang CH, Jayabalan N (2006) Development of an embryogenic suspension culture of bitter melon (Momordica charantia L.). Sci Hortic 109:123–129. doi:10.1016/j.scienta.2006.03.012

    Article  CAS  Google Scholar 

  • Thomas DT (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631

    Article  PubMed  CAS  Google Scholar 

  • Wu YJ, Huang XL, Chen QZ, Li XJ, Engelmann F (2007) Induction and cryopreservation of embryogenic cultures from nucelli and immature cotyledon cuts of mango (Mangifera indica L. var Zihua). Plant Cell Rep 26:161–168

    Article  PubMed  CAS  Google Scholar 

  • Yadava R, Chawla HS (2002) Role of genotypes, growth regulators and amino acids on callus induction and plant regeneration from different developmental stages of inflorescence in wheat. Indian J Genet Plant Breed 62(1):55–60

    CAS  Google Scholar 

  • Zaidi MA, Narayanan M, Sardana R, Taga I, Postel S, Johns R, McNulty M, Mottiar Y, Mao J, Loi E, Altosaar I (2006) Optimizing tissues culture media for efficient transformation of different indica rice genotype. Agron Res 4(2):563–575

    Google Scholar 

  • Zhang CX, Li Q, Kong L (2007) Induction, development and maturation of somatic embryos in Bunge’s pine (Pinus bungeana Zucc. ex Endl.). Plant Cell Tiss Organ Cult 91:273–280

    Article  Google Scholar 

Download references

Acknowledgments

Authors are thankful to the National Centre for Agronomic Research of Ivory Coast for supplying pineapple suckers. We are grateful to Dr. Alison Pawlus for critically reading the manuscript.

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Authors and Affiliations

  1. UFR des Sciences et Technologie des Aliments, Laboratoire de Technologie Alimentaire, Université d’Abobo-Adjamé, 02 BP 801, Abidjan, 02, Côte d’Ivoire

    Edwige Sopie Yapo & Patrice Kouame

  2. UFR des Sciences de la Nature, Laboratoire de Biologie et Amélioration des Productions Végétales, Université d’Abobo-Adjamé, 02 BP 801, Abidjan, 02, Côte d’Ivoire

    Edwige Sopie Yapo, Tanoh Hilaire Kouakou, Mongomaké Kone & Justin Yatty Kouadio

  3. Groupe d’Etude des Substances Végétales à Activités Biologiques, EA 3675, Laboratoire de Mycologie et Biotechnologie Végétale, UFR Pharmacie, Université de Bordeaux 2/Institut des Sciences de la Vigne, et du Vin—CS 50008, 210, Chemin de Leysotte, 33882, Villenave d’Ornon, France

    Tanoh Hilaire Kouakou & Jean-Michel Merillon

Authors
  1. Edwige Sopie Yapo
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  2. Tanoh Hilaire Kouakou
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  3. Mongomaké Kone
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  4. Justin Yatty Kouadio
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  5. Patrice Kouame
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  6. Jean-Michel Merillon
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Correspondence to Tanoh Hilaire Kouakou.

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Yapo, E.S., Kouakou, T.H., Kone, M. et al. Regeneration of pineapple (Ananas comosus L.) plant through somatic embryogenesis. J. Plant Biochem. Biotechnol. 20, 196–204 (2011). https://doi.org/10.1007/s13562-011-0046-5

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  • DOI: https://doi.org/10.1007/s13562-011-0046-5

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