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Coat protein deletion mutation of Mungbean yellow mosaic India virus (MYMIV)

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Abstract

Mungbean yellow mosaic India virus (MYMIV) causing yellow mosaic disease in grain legumes belongs to the genus Begomovirus of the family Geminiviridae. The virus has a bipartite genome comprising two circular single stranded DNA components (DNA A and DNA B) and is transmitted by the whitefly, Bemisia tabaci Genn. DNA A encodes for coat protein on the viral strand and for replication associated proteins and transcription activator proteins in the complementary strand. The coat protein plays key role in whitefly transmission. In the present communication, the regions in coat protein that govern whitefly transmission and systemic spread were mapped by constructing deletion mutations. Results showed that inoculation with viral constructs having N′ terminal deletion of 75 and 150 amino acids affected systemic spread and pathogenicity in cowpea, mungbean and blackgram plants contrasting to French bean which developed symptoms similar to wild type. Assembly of particles and whitefly transmission were not seen in all the mutations. However there was minimum, six folds increase in viral DNA levels in French bean plants inoculated with N′ terminal deletions.

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Abbreviations

MYMIV:

Mungbean yellow mosaic India virus

SLCV:

Squash leaf curl virus

ORF:

Open reading frame

CP:

Coat protein

PTR:

Partial tandem repeat

CTAB:

Cetyl trimethyl ammonium bromide

NBT:

Nitroblue tetrazolium

BCIP:

5-Bromo-4-chloro-3’-indolyl phosphate

ISEM:

Immunosorbent electron microscopy

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Acknowledgement

The financial support given by Indian Council of Agricultural Research “ICAR network project on transgenic in crops, cotton and soybean, Code no.21–25” Government of India is gratefully acknowledged. We are thankful to Dr RK. Jain, Division of Plant Pathology and Dr HS. Gupta, Director, Indian Agricultural Research Institute, New Delhi-110012, India, for providing necessary facilities. We are also grateful to the scientists and staff at National Phytotron Facility, Indian Agricultural Research Institute, New Delhi-110012, India for their guidance and support to grow plants under controlled conditions.

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Authors and Affiliations

  1. Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Q. M. I. Haq, P. Jyothsna & V. G. Malathi

  2. Department of Biotechnology, Jamia Millia Islamia (A Central University), Jamia Nagar, New Delhi, 110025, India

    Q. M. I. Haq & Arif Ali

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  1. Q. M. I. Haq
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  2. P. Jyothsna
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  3. Arif Ali
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  4. V. G. Malathi
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Correspondence to V. G. Malathi.

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Haq, Q.M.I., Jyothsna, P., Ali, A. et al. Coat protein deletion mutation of Mungbean yellow mosaic India virus (MYMIV). J. Plant Biochem. Biotechnol. 20, 182–189 (2011). https://doi.org/10.1007/s13562-011-0044-7

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  • DOI: https://doi.org/10.1007/s13562-011-0044-7

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