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Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants


Jatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant, however, oil and deoiled cake are toxic. A non-toxic variety of J. curcas is reported from Mexico. The present investigation explores the effects of different plant growth regulators (PGRs) viz. 6-benzyl aminopurine (BAP) or thidiazuron (TDZ) individually and in combination with indole-3-butyric acid (IBA), on regeneration from in vitro and field-grown mature leaf explants, in vitro and glasshouse-grown seedlings cotyledonary leaf explants of non-toxic J. curcas. In all the tested parameters maximum regeneration efficiency (81.07%) and the number of shoot buds per explants (20.17) was observed on 9.08 μM TDZ containing Murashige and Skoog’s (MS) medium from in vitro cotyledonary leaf explants. The regenerated shoot buds were transferred to MS medium containing 10 μM kinetin (Kn), 4.5 μM BAP and 5.5 μM α-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with 2.25 μM BAP and 8.5 μM IAA. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA and NAA for four days followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg/l activated charcoal. The rooted plants could be established in soil with more than 90% survival rate.

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Fig. 1





Indole-3-acetic acid


Indole-3-butyric acid




Murashige and Skoog


Naphthaleneacetic acid




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The authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi for financial assistance and Prof. K. Becker, Department of Aquaculture Systems and Animal Nutrition, University of Hohenheim, Stuttgart, Germany for providing Mexican non-toxic J. curcas seeds. Statistical data analysis support from Dr. Keyur Shah and Dr. Amritpal Singh, Assistant professor, Anand Agricultural University, Anand is thankfully acknowledged.

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Correspondence to Nitish Kumar.

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Kumar, N., Vijay Anand, K.G. & Reddy, M.P. Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants. J. Plant Biochem. Biotechnol. 20, 125–133 (2011).

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  • Non-toxic Jatropha curcas
  • Leaf
  • Plant growth regulator
  • Regeneration