Analysis of transgene(s) (psy+crtI) inheritance and its stability over generations in the genetic background of indica rice cultivar Swarna
- 143 Downloads
Rice the major staple food crop which feeds more than half of the world’s population but, lacks pathway to synthesize and accumulate provitamin A in endosperm therefore rice eaters particularly children, and pregnant women suffer due to vitamin A deficiency. The pathway for provitamin A synthesis in rice endosperm has been engineered and transgenic rice lines have yellow endosperm, called ‘Golden Rice’. The present study aimed at studying the inheritance of transgene(s) in six transgenic events of ‘Golden Rice’ and transfer of provitamin A trait from transgenic lines to a widely grown mega rice variety Swarna. The events E1, R1 and W1 showed normal Mendelian inheritance in F2, BC1F1 and BC1F2 generations. The event W1 was studied in BC1F3 as well and showed normal Mendelian inheritance of 3:1. The inheritance pattern in L1 event in BC1F1 and BC1F2 showed normal Mendelian inheritance following expected ratio 1:1 and 3:1 respectively. The two events G1 and T1 showed distorted segregation in BC1F2 and BC2F2 respectively in Swarna genetic background. In G1 event, transgene inheritance showed segregation distortion in BC1F2 in favour of transgene negative plants. In T1 event, inheritance followed expected Mendelian segregation in BC1F1, BC2F1 and BC2F2, generations. However, when tested against co-dominant inheritance 1:2:1 pattern in BC2F2, segregation distortion was observed with less than the expected transgene homozygotes. While against 3:1 ratio, it showed the expected segregation pattern in BC2F2 generation. Segregation distortion probably due to differential transmission of transgene positive/negative gametes through either/both parents which needs further study.
KeywordsProvitamin A Transgenic event Phytoene synthase (psy) Phytoene desaturase (crtI) Transgene inheritance Segregation distortion
Vitamin A Deficiency
Geranyl geranyl Pyrophosphate
Polymerase chain reaction
National Phytoron Facility
Indian Agricultural Research Institute
National Bureau of Plant Genetic Resources
The first author sincerely acknowledges the financial assistance he received from Central Scientific and Industrial Research (CSIR) in the form of Senior Research Fellowship for conducting his doctoral research. This work was carried out as a part of the Network Project on Golden rice funded by Department of Biotechnology, Government of India.
- Burkhardt PK, Beyer P, Wunn J, Kloti A, Armstrong GA, Schledz M, von Lintig V, Potrykus I (1997) Transgenic rice (Oyza stiva) endosperm expressing daffodil (Nacissus psecudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J 11:1071–1078PubMedCrossRefGoogle Scholar
- Christian P, West KP Jr, Khatry SK, Kimbrough-Pradhan E, LeClerq SC, Katz J, Shrestha SR, Dali SM, Sommer A (2000) Night blindness during pregnancy and subsequent mortality among women in Nepal: effects of vitamin A and β-carotene supplementation. Am J Epidemiol 152:542–547PubMedCrossRefGoogle Scholar
- Nandakumar N, Tyagi NK, Balram M, Chikkappa GK, Aprajita G, Mohapatra T, Prabhu KV, Singh AK (2008) Molecular analysis of transgene (psy) inheritance in a golden rice line developed in the genetic background of IR64. J Plant Biochem Biotech 17(2):127–132Google Scholar
- Satoto Sulistyowati Y, Hartanac A, Slamet-Loedin IH (2008) The segregation pattern of insect resistance genes in the progenies and crosses of transgenenic Rojolele Rice. Indonesian Journal of Agricultural Science 9(2):35–43Google Scholar