In recent years the mechanism of action of LLLT has been established. Neira et al.  examined the effect of LLLT at 635 nm with 10 mW intensity on human adipose tissue taken from lipectomy samples. Tissue samples were irradiated for 6 min and compared with non-irradiated samples. Utilizing scanning and transmission electron microscopy (SEM and TEM) more than 180 images were collected, and revealed that 99% of the cellular content including fat was released from the adipocyte, a phenomenon not observed within the control samples. Further, TEM images of the adipose tissue were taken at 60,000× and revealed the formation of transitory pore and complete deflation of adipocytes . Over this effect, LLLT at 635 nm has biochemical activity on adipocytes. The biochemical effects of low-level laser energy laser on adipose tissue appears to be mediated through an effect on cytochrome C oxidase, a large mitochondrial membrane enzyme that facilitates the transfer of electrons from cytochrome C to oxygen. In detail, LLLT stimulates the mitochondria in adipocytes to increase adenosine triphosphate (ATP) synthesis with subsequent up regulation of cAMP. The increase of cAMP stimulates cytoplasmic lipase, which converts triglycerides into fatty acids and glycerol that can pass through pores formed in the cell membrane . Following the application of low laser energy, fatty acids, glycerol, and triglycerides pass across the membrane through these transitory pores and into extracellular space resulting in complete adipocytes deflation.
Training using vibration platforms is a method of neuromuscular activation utilized for the development of strength and power. These vibrations transmit themselves through the body, causing a notable stimulation of sensorial receptors, principally the neuromuscular spindles. Recent studies have demonstrated that brief exposures to this type of treatment causes an acute increase in the muscular capacity of the subject, in both the upper  and lower  extremities. It has also been observed to produce an increase in the consumption of oxygen (O2), of cardiac frequency (FC) and of the subjective perception of strength (RPE), similar to that produced with moderate resistance training .
In this study, we have tested the efficacy of a combination treatment with LLLT and vibration therapy for the treatment of localized adiposity and fibrous cellulite. Indeed, we have been able to demonstrate good results for the two pathological conditions, both at subjective and objective level.
Histopathological analysis has confirmed a significant reduction of the thickness of the cutaneous fat in the skin area treated with LLLT, with good preservation of the anatomical structures. In fact connective tissue, blood vessels and nerves had no observable damage by hematoxylin and eosin and Van Gieson trichromic staining techniques.
Ecographic evaluation confirmed the results of the histological exam, showing a measurable reduction of the fat thickness.
LLLT is a treatment able to increase the adipocyte metabolism with the consequent mobilization of the intracellular lipids. However, if not adequately metabolized, the lipids tend to re-set down invalidating the long-term effects of the treatment, especially in the absence of a correct lifestyle. Our data suggest that the combination of LLLT with vibration therapy is able to stimulate a metabolic and muscle stimulation, that are efficient in the consumption of the energy derived from the catabolism of the lipids mobilized by LLLT. Furthermore, the vibration therapy has also a strengthening effect on the skin.
The purpose of the study was to emphasize the effectiveness and safety of the combination of the two methods: LLLT and oscillating vibration technology. The sense of this combination arises from an attempt to improve the already satisfactory results of LLLT alone, as demonstrated in the literature, and to facilitate the use of mobilized free fatty acids following the adipocyte “depletion” induced by laser lipolysis. The oscillating vibration technology is not able to directly induce lipolysis, but provokes exclusively the basic processes involved in the pathogenetic mechanism of localized adiposity and cellulite . Furthermore, it is able to stimulate the enzymatic processes involved in energy metabolism and to stimulate endothelial function and muscle due to stimulation of alpha motor neurons, resulting in an increase of muscle activity  and stimulation of O2 consumption, and of the basal metabolic rate . Thus, in patients subjected to the combined therapy, such are those involved in this study, it was not essential to do physical exercise or follow a low-calorie/low-fat diet, in order to ensure the effectiveness and safety of the treatment.
Finally, blood tests have demonstrated the safety of the treatment. Indeed, all patients showed a slight decrease in VLDL and triglycerides. It is reasonable to hypothesize that the combination of LLLT with vibration therapy is able to induce an increase of the basal metabolism, which in turn could promote the consumption of the lipids mobilized from the fat tissue. Finally, we have never detected an increase in GOT and GPT levels, thus excluding any deleterious effect on the hepatic metabolism.
The relevant limitations of this study include: (1) metabolic data presented are sufficient to exclude toxic effects of the treatment, but are not enough to perfectly determine the biochemical events involved in the increase of the basal metabolism, that could be responsible, at least in part, for the effects on cellulite reduction; and (2) only one biopsy has been analyzed to investigate at the morphological effects of the treatment. This causes a limitation in the interpretation of the results.
In conclusion, the combination between LLLT and vibration therapy is an effective treatment for resolution of localized adiposity and fibrous cellulite. Moreover, the action of Vibro Light apparatus is safe, effective, and well tolerated as a procedure for body contouring. In particular, the study suggests that the combination treatment between LLLT and vibration therapy, using Vibro Light, could be an ideal non-invasive alternative to conventional liposuction for patients who require only small or moderate removal of adipose tissue or cellulite, and above all for those patients that refuse changes in their lifestyle through hyperproteic diet or appropriate physical activities. Considering the limitations of the study, further studies including a higher number of patients are required in order to better define the metabolic consequences of this treatment and the histological effects.