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Application of Electron Spin Resonance Spectroscopy in Bone Regeneration Studies

  • General and Applied Physics
  • Published:
Brazilian Journal of Physics Aims and scope Submit manuscript

Abstract

Electron spin resonance (ESR) is a spectroscopic technique that detects unpaired electrons, such as the ones present in free radicals. When a calcified tissue is irradiated, free radicals are generated, like the CO2−. This paper presents a preliminary study on the application of this spectroscopic technique for monitoring bone mineralization processes during the bone repair in animal model, induced by polyurethane in two forms, as an occlusive membrane and as a bone grafting. In the first experiment, the ESR results and microscopic analysis demonstrate centripetal bone growth and advanced stage of bone regeneration in the treated group. In the second experiment, the ESR spectrum of collected bone pieces presents the superposition of the spectrum of the biomaterial and the radical CO2−. The separation of the components was made using spectral simulation, and the percentage of mature bone tissue in the defect region was determined through the comparison with the spectrum of the native bone. In both experiments, ESR provided results consistent with microscopic analysis, demonstrating the feasibility of the technique for monitoring bone regeneration.

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References

  1. M. Murshed, Cold Spring Harb. Perspect. Med. 8, 1 (2018)

    Article  Google Scholar 

  2. L. J. Raggatt, N. C. Partridge, 285, 25103 (2010)

  3. J. R. Cameron, J. Sorenson, Science (80-. ). 142, 230 (1963)

  4. P.D. Miller, Bone 104, 4 (2017)

    Article  Google Scholar 

  5. P. Fattibene, F. Callens, Appl. Radiat. Isot. 68, 2033 (2010)

    Article  Google Scholar 

  6. F. Callens, R. Verbeeck, P. Matthys, L. Martens, E. Boesman, Calcif. Tissue Int. 41, 124 (1987)

    Article  Google Scholar 

  7. S. Mascarenhas, A. Hasegawa, K. Takeshita, Bull Am Phys Soc 18, 579 (1973)

    Google Scholar 

  8. A. Kinoshita, O. Baffa, S. Mascarenhas, PLoS One 13, e0192444 (2018)

  9. IAEA, Use of Electron Paramagnetic Resonance Dosimetry with Tooth Enamel for Retrospective Dose Assessment (TECDOC Series, 2002). https://www-pub.iaea.org/MTCD/Publications/PDF/te_1331_web.pdf. Accessed 25 Jan 2022

  10. A. Kinoshita, F. Braga, C.F.O. Graeff, O. Baffa, Appl. Radiat. Isot. 54, 269 (2001)

    Article  Google Scholar 

  11. M. Ikeya, J. Miyajima, S. Okajima, Jpn. J. Appl. Phys. 23, L697 (1984)

    Article  ADS  Google Scholar 

  12. C. Fainstein, Brazilian. J. Phys. 36, 90 (2006)

    Google Scholar 

  13. M. Ikeya, New applications of electron spin resonance: Dating, dosimetry and microscopy (World Scientific, New Jersey, USA, 1993)

    Book  Google Scholar 

  14. S. Mascarenhas, O.B. Filho, M. Ikeya, Am. J. Phys. Anthropol. 59, 413 (1982)

    Article  Google Scholar 

  15. L. dos S. Avilla, A. M. Graciano Figueiredo, A. Kinoshita, C. Bertoni-Machado, D. Mothé, L. Asevedo, O. Baffa, V. H. Dominato, Quat. Int. 305, 85 (2013)

  16. R. L. Azevedo, V. K. Asfora, D. S. Mützenberg, D. Cisneiros, H. L. Sullasi, A. M. Kinoshita, P. L. Guzzo, A. R. Skinner, O. Baffa, A.-M. Pessis, H. J. Khoury, Quat. Int. 556, (2020)

  17. A. Kinoshita, L. Figuty, O. Baffa, Brazilian. J. Phys. 36, 93 (2006)

    Google Scholar 

  18. A. Kinoshita, H. L. Sullasi, V. K. Asfora, R. L. Azevedo, P. Guzzo, N. Guidon, A. G. F. Maria, H. Khoury, A.-M. Pessis, O. Baffa, An. Acad. Bras. Cienc. 88, (2016)

  19. A. Kinoshita, A. R. Skinner, N. Guidon, E. Ignacio, G. D. Felice, C. D. a Buco, S. Tatumi, M. Yee, A. M. G. Figueiredo, O. Baffa, J. Hum. Evol. 77C, 187 (2014)

  20. S. Watanabe, W. E. F. Ayta, H. Hamaguchi, N. Guidon, E. S. La Salvia, S. Maranca, O. Baffa Filho, J. Archaeol. Sci. 30, 351 (2003)

  21. R. P. Lopes, J. C. Pereira, A. Kinoshita, M. Mollemberg, F. Barbosa, O. Baffa, J. South Am. Earth Sci. 101, 102605 (2020)

  22. R. P. Lopes, J. C. Pereira, S. R. Dillenburg, S. H. Tatumi, M. Yee, A. M. G. Figueiredo, A. Kinoshita, O. Baffa, J. South Am. Earth Sci. 100, 102566 (2020)

  23. J. A. McGovern, M. Griffin, D. W. Hutmacher, Dis. Model. Mech. 11, (2018)

  24. R.I. Falacho, P.J. Palma, J.A. Marques, M.H. Figueiredo, F. Caramelo, I. Dias, C. Viegas, F. Guerra, Molecules 26, 1339 (2021)

    Article  Google Scholar 

  25. Y. Li, S.-K. Chen, L. Li, L. Qin, X.-L. Wang, Y.-X. Lai, J. Orthop. Transl. 3, 95 (2015)

    Google Scholar 

  26. V.M.R. Barros, A.L. Rosa, M.M. Beloti, G. Chierice, J. Biomed. Mater. Res. Part A 67A, 235 (2003)

    Article  Google Scholar 

  27. O.C.M. Pereira-Júnior, S.C. Rahal, P. Iamaguti, S.L. Felisbino, P.T. Pavan, L.C. Vulcano, J. Biomater. Appl. 21, 283 (2007)

    Article  Google Scholar 

  28. P. Gentile, V. Chiono, C. Tonda-Turo, A.M. Ferreira, G. Ciardelli, Biotechnol. J. 6, 1187 (2011)

    Article  Google Scholar 

  29. C. Dahlin, E. Sandberg, P. Alberius, A. Linde, Int. J. Oral Maxillofac. Surg. 23, 237 (1994)

    Article  Google Scholar 

  30. D. D. Bosshardt, R. K. Schenk, in 20 Years Guid. Bone Regen. Implant Dent., ed. by D. Buser, 2nd ed. (Quintessence, Chicago, 2010), pp. 15–46

  31. F.J. O’Brien, Mater. Today 14, 88 (2011)

    Article  Google Scholar 

  32. T. P. T. De Sousa, M. S. T. Da Costa, R. Guilherme, W. Orcini, L. de A. Holgado, E. M. Varize Silveira, O. Tavano, A. G. Magdalena, S. A. Catanzaro-Guimarães, A. Kinoshita, Polimeros 28, 246 (2018)

  33. G. C. Belmonte, S. A. Catanzaro-Guimarães, T. P. T. De Sousa, R. S. Carvalho, A. Kinoshita, G. O. Chierici, Polimeros 23, (2013)

  34. R. R. C. Da Costa, F. R. B. De Almeida, A. A. X. Da Silva, S. M. Domiciano, A. F. C. Vieira, Polimeros 29, (2019)

  35. I. Chakraborty, K. Chatterjee, Biomacromol 21, 4639 (2020)

    Article  Google Scholar 

  36. O. Baffa, J.R. Olivieri, S. Mascarenhas, O.R. Nascimento, Brazilian J. Med. Biol. Res. 16, 396 (1983)

    Google Scholar 

Download references

Acknowledgements

The authors are grateful to Professor Sergio Mascarenhas to whom we dedicate this article and who, through his pioneering work in the 1970s on physical aspects of hard tissues, such as bones and teeth, which resulted in several articles, theses and dissertations, drew attention to this area of interdisciplinary research.

Funding

This work received funding from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Grants 03/09505–6, 07/06720–4 & 2013/07699–0, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Grants 304107/2019–0 and 309186/2020–0 and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) código 001.

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Authors and Affiliations

  1. Centro Universitário Sagrado Coração, Unisagrado, Bauru, 17.011-160, São Paulo, Brazil

    Gisele Janaina Blasioli, Leandro Andrade Holgado & Tatiana Peixoto Telles de Sousa

  2. Universidade Do Oeste Paulista, UNOESTE, Presidente Prudente, 19067-175, São Paulo, Brazil

    Lucas Henrique Pereira Silva & Angela Kinoshita

  3. Instituto Federal de São Paulo, Presidente Epitácio, SP, São Paulo, Brazil

    Lucas Henrique Pereira Silva

  4. FOB-Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, 17012-90, São Paulo, Brazil

    Sergio Augusto Catanzaro Guimarães

  5. Departamento de Física, FFCLRP, Universidade São Paulo, Ribeirão Preto, 14049-900, São Paulo, Brazil

    Oswaldo Baffa

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  1. Gisele Janaina Blasioli
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  2. Lucas Henrique Pereira Silva
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  3. Leandro Andrade Holgado
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  6. Oswaldo Baffa
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  7. Angela Kinoshita
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Correspondence to Angela Kinoshita.

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The authors declare that they have no conflict of interest with any organization regarding the material discussed in this manuscript. This work was partially supported by the research funding agencies as mentioned and it was conducted in accordance with ethical academic standards and also respecting ethical guidance for animal studies. The preliminary version of this work was presented at the 18th International Conference on Medical Physics (April 17-20, 2011 Porto Alegre - RS - Brazil) and an abstract was published.

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Blasioli, G.J., Silva, L.H.P., Holgado, L.A. et al. Application of Electron Spin Resonance Spectroscopy in Bone Regeneration Studies. Braz J Phys 52, 51 (2022). https://doi.org/10.1007/s13538-022-01060-4

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