Abstract
The current study aimed to develop a potential wound dressing using vitamin B12-loaded polycaprolacton/gelatin nanofibrous scaffold. In order to produce wound dressings, 1000 mcg of vitamin B12 was added to polycaprolacton/gelatin solution and the nanofibrous scaffolds were fabricated through electrospinning method. The obtained scaffolds were studied regarding their hydrophobicity, microstructure, amount of water absorption, water vapor permeability, tensile strength, release test, and cellular proliferation assay. In vitro studies revealed that the incorporation of vitamin b12 into polycaprolacton/gelatin scaffolds could significantly augment L929 cells proliferation at 1 and 3 days post-seeding. However, there was not statistically significant difference between Vitamin B12-containing and polymer-only scaffolds in tensile strength study, surface wettability measurement, water vapor transmission test, the capacity for water absorption, and nanofiber’s diameter. Both vitamin containing and free dressings were applied on the full-thickness excisional wound in rat model to compare their healing potential. Our results showed that after 14 days, vitamin B12 containing dressing could significantly enhance wound closure compared to vitamin B12 free scaffolds (92.27 ± 6.84% vs. 64.62 ± 2.96%). Furthermore, histopathological examinations showed significantly greater epithelial thickness in polycaprolacton/gelatin/vitamin B12 group compared to other experimental groups. This preliminary study suggest potential applicability of the proposed dressing to treat skin wounds in clinic.
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References
Stojic M, et al. Skin tissue engineering. In: García-Gareta E, editors. Biomaterials for skin repair and regeneration. Amsterdam: Elsevier; 2019, p. 59-99.
Qu J, et al. Degradable conductive injectable hydrogels as novel antibacterial, anti-oxidant wound dressings for wound healing. Chem Eng J. 2019;362:548–60.
Zahedi P, et al. A review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages. Polym Adv Technol. 2010;21(2):77–95.
Bognitzki M, et al. Nanostructured fibers via electrospinning. Adv Mater. 2001;13(1):70–2.
Subbiah T, et al. Electrospinning of nanofibers. J Appl Polym Sci. 2005;96(2):557–69.
Zhao R, et al. Electrospun chitosan/sericin composite nanofibers with antibacterial property as potential wound dressings. Int J Biol Macromol. 2014;68:92–7.
Gamez E, et al. Antimicrobial electrospun polycaprolactone-based wound dressings: an in vitro study about the importance of the direct contact to elicit bactericidal activity. Adv Wound Care. 2019;8(9):438–51.
Xue J, et al. Bioinspired multifunctional biomaterials with hierarchical microstructure for wound dressing. Acta Biomater. 2019;100:270–9.
Heidari M, et al. Smart electrospun nanofibers containing PCL/gelatin/graphene oxide for application in nerve tissue engineering. Mater Sci Eng C. 2019;103:109768.
Gil-Castell O, et al. Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation. Mater Sci Eng C. 2020;107:110296.
Bahcecioglu G, et al. Hydrogels of agarose, and methacrylated gelatin and hyaluronic acid are more supportive for in vitro meniscus regeneration than three dimensional printed polycaprolactone scaffolds. Int J Biol Macromol. 2019;122:1152–62.
Hu Y, et al. Electrospun gelatin/PCL and collagen/PCL scaffolds for modulating responses of bone marrow endothelial progenitor cells. Exp Therap Med. 2019;17(5):3717–26.
Kannaiyan J, et al. Fabrication of electrospun polycaprolactone/gelatin composite nanofibrous scaffolds with cellular responses. Am J Nano Res Appl. 2019;7(2):11–20.
Mendonça N, et al. Plasma vitamin B12, supplementation and mortality. J Gerontol Ser A. 2019;74(1):138–8.
Rathod RS, et al. Maternal omega-3 fatty acid supplementation to a vitamin B12 deficient diet normalizes angiogenic markers in the pup brain at birth. Int J Dev Neurosci. 2015;43:43–9.
Aroni K, et al. Skin hyperpigmentation and increased angiogenesis secondary to vitamin B12 deficiency in a young vegetarian woman. Acta Derm Venereol. 2008;88(2):191–2.
Ehterami A, et al. In vitro and in vivo study of PCL/COLL wound dressing loaded with insulin-chitosan nanoparticles on cutaneous wound healing in rats model. Int J Biol Macromol. 2018;117:601–9.
Sood A, Granick MS, Tomaselli NL. Wound dressings and comparative effectiveness data. Adv Wound Care. 2014;3(8):511–29.
Salehi M, et al. Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled-releasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit. J Biomed Mater Res Part B Appl Biomater. 2018;106(4):1463–76.
Yu Y, et al. Vitamin metal-organic framework-laden microfibers from microfluidics for wound healing. Mater Horiz. 2018;5(6):1137–42.
Mohammed BM, et al. Vitamin C promotes wound healing through novel pleiotropic mechanisms. Int Wound J. 2016;13(4):572–84.
Starr NJ, et al. Enhanced vitamin C skin permeation from supramolecular hydrogels, illustrated using in situ ToF-SIMS 3D chemical profiling. Int J Pharm. 2019;563:21–9.
Batchelor R, et al. (–)-Riboflavin (vitamin B2) and flavin mononucleotide as visible light photo initiators in the thiol-ene polymerisation of PEG-based hydrogels. Polym Chem. 2017;8(6):980–4.
Li H, et al. Electrospun gelatin nanofibers loaded with vitamins A and E as antibacterial wound dressing materials. RSC Adv. 2016;6(55):50267–77.
Najafi-Taher R, et al. Preparation of an ascorbic acid/PVA-chitosan electrospun mat: a core/shell transdermal delivery system. RSC Adv. 2015;5(62):50462–9.
Madhaiyan K, et al. Vitamin B12 loaded polycaprolactone nanofibers: a novel transdermal route for the water soluble energy supplement delivery. Int J Pharm. 2013;444(1–2):70–6.
Abubakr N, et al. Effects of encapsulation process parameters of calcium alginate beads on Vitamin B12 drug release kinetics. Asia Pac J Chem Eng. 2010;5(5):804–10.
Kim G, et al. Effects of vitamin B12 on cell proliferation and cellular alkaline phosphatase activity in human bone marrow stromal osteoprogenitor cells and UMR106 osteoblastic cells. Metabolism. 1996;45(12):1443–6.
Urban K, et al. Influence of B vitamins on proliferation and differentiation of osteoblastic bovine cell cultures: an in vitro study. In: Urban K, Auer J, Bürklein S, Plate U, editors. Biomineralization. Springer; 2018. p. 121–8.
Cantatore P, et al. Alteration of mitochondrial DNA and RNA level in human fibroblasts with impaired vitamin B12 coenzyme synthesis. FEBS Lett. 1998;432(3):173–8.
Wang Y-P, et al. High frequencies of vitamin B12 and folic acid deficiencies and gastric parietal cell antibody positivity in oral submucous fibrosis patients. J Formos Med Assoc. 2015;114(9):813–9.
Rembe J-D, Fromm-Dornieden C, Stuermer EK. Effects of vitamin B complex and vitamin C on human skin cells: is the perceived effect measurable? Adv Skin Wound Care. 2018;31(5):225–33.
Saghiri MA, et al. Vitamins and regulation of angiogenesis:[A, B1, B2, B3, B6, B9, B12, C, D, E, K]. J Funct Foods. 2017;38:180–96.
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This study was supported by AJA University of Medical Sciences, Tehran, Iran.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of institutional research committee. Informed consent was obtained from all participants included in the study.
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Farzanfar, S., kouzekonan, G.S., Mirjani, R. et al. Vitamin B12-loaded polycaprolacton/gelatin nanofibrous scaffold as potential wound care material. Biomed. Eng. Lett. 10, 547–554 (2020). https://doi.org/10.1007/s13534-020-00165-6
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DOI: https://doi.org/10.1007/s13534-020-00165-6