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Light sheet microscopy for histopathology applications

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Abstract

Light sheet microscopy (LSM) is an evolving optical imaging technique with a plane illumination for optical sectioning and volumetric imaging spanning cell biology, embryology, and in vivo live imaging. Here, we focus on emerging biomedical applications of LSM for tissue samples. Decoupling of the light sheet illumination from detection enables high-speed and large field-of-view imaging with minimal photobleaching and phototoxicity. These unique characteristics of the LSM technique can be easily adapted and potentially replace conventional histopathological procedures. In this review, we cover LSM technology from its inception to its most advanced technology; in particular, we highlight the human histopathological imaging applications to demonstrate LSM’s rapid diagnostic ability in comparison with conventional histopathological procedures. We anticipate that the LSM technique can become a useful three-dimensional imaging tool for assessing human biopsies in the near future.

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Acknowledgements

This research was supported by a GIST Research Institute grant funded by GIST in 2019 and grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (NRF-2016R1A2B4015381), by the Brain Research Program through the NRF funded by the Ministry of Science, ICT & Future Planning (NRF-2017M3C7A1044964), and also by the Bio & Medical Technology Development Program of NRF funded by the Korean government (MSIT) (NRF-2015M3A9E2030125). The authors thank Mr. Ripon Kumar Saha for preparing schematic drawings.

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  1. Department of Biomedical Science and Engineering (BMSE), Gwangju Institute of Science and Technology (GIST), Gwangju, 61005, Republic of Korea

    Praveen Kumar Poola, Muhammad Imran Afzal, Youngseung Yoo & Euiheon Chung

  2. Institute of Integrated Technology, GIST, Gwangju, 61005, Republic of Korea

    Euiheon Chung

  3. School of Mechanical Engineering, GIST, Gwangju, 61005, Republic of Korea

    Euiheon Chung

  4. Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Republic of Korea

    Ki Hean Kim

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  1. Praveen Kumar Poola
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Correspondence to Euiheon Chung.

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Poola, P.K., Afzal, M.I., Yoo, Y. et al. Light sheet microscopy for histopathology applications. Biomed. Eng. Lett. 9, 279–291 (2019). https://doi.org/10.1007/s13534-019-00122-y

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