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Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

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Abstract

This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation via decellularized human amniotic membrane (DAM), for the promotion of skin excisional wound repair. The DAM was seeded with MenSCs at the density of 3 × 104 cells/cm2 and implanted onto a rat’s 1.50 × 1.50 cm2 full-thickness excisional wound defect. The results of wound closure and histopathological examinations demonstrated that the MenSC-seeded DAM could significantly improve the wound healing compared with DAM-treatment. All in all, our data indicated that the MenSCs can be a potential source for cell-based therapies to regenerate skin injuries.

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Author information

Author notes

  1. Saeed Farzamfar, Majid Salehi, Arian Ehterami and Mahdi Naseri-Nosar are co-first authors and contributed equally to this work.

Authors and Affiliations

  1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Saeed Farzamfar & Ahmad Vaez

  2. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Majid Salehi & Arian Ehterami

  3. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran

    Majid Salehi & Mahdi Naseri-Nosar

  4. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Amir Hassan Zarnani & Mehdi Aleahmad

  5. Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

    Amir Hassan Zarnani

  6. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Hamed Sahrapeyma

  7. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mohammad-Reza Shokri

Authors
  1. Saeed Farzamfar
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  2. Majid Salehi
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  3. Arian Ehterami
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  4. Mahdi Naseri-Nosar
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  5. Ahmad Vaez
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  6. Amir Hassan Zarnani
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  7. Hamed Sahrapeyma
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  8. Mohammad-Reza Shokri
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  9. Mehdi Aleahmad
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Corresponding author

Correspondence to Majid Salehi.

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The authors declare that they have no conflict of interest.

Ethical statement

Animal experiments were approved by the ethical committee of Shahroud University of Medical Sciences and were carried out in accordance with the university’s guidelines.

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Farzamfar, S., Salehi, M., Ehterami, A. et al. Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane. Biomed. Eng. Lett. 8, 393–398 (2018). https://doi.org/10.1007/s13534-018-0084-1

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  • DOI: https://doi.org/10.1007/s13534-018-0084-1

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