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First demonstration of velocity selective recording from the pig vagus using a nerve cuff shows respiration afferents

Abstract

Neural interfaces have great potential to treat disease and disability by modulating the electrical signals within the nervous system. However, whilst neural stimulation is a well-established technique, current neural interfaces are limited by poor recording ability. Low signal amplitudes necessitate the use of highly invasive techniques that divide or penetrate the nerve, and as such are unsuitable for chronic implantation. In this paper, we present the first application of the velocity selective recording technique to the detection of respiration activity in the vagus nerve, which is involved with treatments for epilepsy, depression, and rheumatoid arthritis. Further, we show this using a chronically implantable interface that does not divide the nerve. We also validate our recording setup using electrical stimulation and we present an analysis of the recorded signal amplitudes. The recording interface was formed from a cuff containing ten electrodes implanted around the intact right vagus nerve of a Danish Landrace pig. Nine differential amplifiers were connected to adjacent electrodes, and the resulting signals were processed to discriminate neural activity based on conduction velocity. Despite the average single channel signal-to-noise ratio of − 5.8 dB, it was possible to observe distinct action potentials travelling in both directions along the nerve. Further, contrary to expectation given the low signal-to-noise ratio, we have shown that it was possible to identify afferent neural activity that encoded respiration. The significance of this is the demonstration of a chronically implantable method for neural recording, a result that will transform the capabilities of future neuroprostheses.

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Correspondence to B. W. Metcalfe.

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We wish to declare that we have no commercial affiliations, consultancies, stock or equity interests, or patent-licensing arrangements that could be considered to pose a conflict of interest regarding the submitted manuscript.

Ethical approval

All animal procedures were performed in accordance with the Danish Animal Experiments Inspectorate (approval no. 2013-15-2934-00753), as well as the care and use of laboratory animals as described by the U.S National Institutes of Health.

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Metcalfe, B.W., Nielsen, T.N., Donaldson, N.N. et al. First demonstration of velocity selective recording from the pig vagus using a nerve cuff shows respiration afferents. Biomed. Eng. Lett. 8, 127–136 (2018). https://doi.org/10.1007/s13534-017-0054-z

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Keywords

  • ENG
  • VSR
  • Neural interface
  • MEC
  • CAP