Skip to main content
SpringerLink
Log in

Gene expression regulating the cell cycle and gene of antioxidant protection system in toxic hepatitis

  • Rapid Communication
  • Published:
Toxicology and Environmental Health Sciences Aims and scope Submit manuscript

Abstract

Background

In the present study, we examined the changes in gene expression regulating the cell cycle and the gene of antioxidant protection system in carbon tetrachloride-induced toxic hepatitis after 24 and 72 h of treatment with carbon tetrachloride.

Objective

Toxic hepatitis was modeled in male albino mongrel rats divided into seven groups depending on the dose of carbon tetrachloride administered. After 24 and 72 h, the rats were anesthetized and mRNA levels of Ripk1, Cdkn1a, Gclc genes were examined in a liver homogenate.

Results

There was a significant dose-dependent change in the Ripk1 gene expression after 24 and 72 h of carbon tetrachloride administration (p < 0.001), as well as a dose-dependent change in the expression of the Cdkn1a (p < 0.05) and Gclc (p < 0.001) genes on the 3rd day after the carbon tetrachloride administration.

Conclusion

This data may indicate the involvement of the investigated genes in carbon tetrachloride metabolism in liver cells at various stages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Asrani SK, Devarbhavi H, Eaton J et al (2019) Burden of liver diseases in the world. J Hepatol 70(1):151–171

    Article  Google Scholar 

  2. Antonenko OM (2013) Toxic liver lesions: ways of pharmacological correction. Meditsinskiy Sovet 6:45–51

    Google Scholar 

  3. Bolshakov OP et al (2008) Didactic and ethic aspects of conducting studies on biomodels and laboratory animals. Quality Clin Practice 1:58–61

    Google Scholar 

  4. Buitrago-Molina LE, Marhenke S, Longerich T et al (2013) The degree of liver injury determines the role of p21 in liver regeneration and hepatocarcinogenesis in mice. Hepatology 58(3):1143–1152

    Article  CAS  Google Scholar 

  5. Espinosa-Díez C, Miguel V et al (2018) Role of glutathione biosynthesis in endothelial dysfunction and fibrosis. Redox Biol 14:88–99

    Article  Google Scholar 

  6. Cheng ML, Lu YF, Chen H et al (2015) Liver expression of Nrf2-related genes in different liver diseases. Hepatobiliary Pancreat Dis Int 14(5):485–491

    Article  Google Scholar 

  7. Dillon CP, Weinlich R, Rodriguez DA et al (2014) RIPK1 blocks early postnatal lethality mediated by caspase-8 and RIPK3. Cell 157(5):1189–1202

    Article  CAS  Google Scholar 

  8. Ehedego H, Trautwein C (2016) p21 in chronic and acute liver injury. Oncoscience 3(2):56

    Article  Google Scholar 

  9. Fan X, Jiang Y, Wang Y et al (2014) Wuzhi tablet (Schisandrasphenanthera extract) protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of NRF2-ARE and p53/p21 pathways. Drug Metab Dispos 42(12):1982–1990

    Article  Google Scholar 

  10. Feoktistova M, Leverkus M (2015) Programmed necrosis and necroptosis signalling. FEBS J 282(1):19–31

    Article  CAS  Google Scholar 

  11. Filliol A, Piquet-Pellorce C, Le Seyec J et al (2016) RIPK1 protects from TNF-α-mediated liver damage during hepatitis. Cell Death Disease 7(11):e2462

    Article  CAS  Google Scholar 

  12. Golikov SN, Sanotskiy IV, Tiunov LA (1986) General mechanisms of toxic effects. L.: Meditsina, pp 54–57

  13. Hydbring P, Malumbres M, Sicinski P (2016) Non-canonical functions of cell cycle cyclins and cyclin-dependent kinases. Nat Rev Mol Cell Biol 17(5):280–292

    Article  CAS  Google Scholar 

  14. Karimian A, Ahmadi Y, Yousefi B (2016) Multiple functions of p21 in cell cycle, apoptosis and transcriptional regulation after DNA damage. DNA Repair 42:63–71

    Article  CAS  Google Scholar 

  15. Kravchenko LV, Trusov NV, Uskova MA et al (2009) Characteristics of acute toxic effects of four chlor carbon as a model of oxidative stress. Toxicologicheskiy Vestnik 1:12–18

    Google Scholar 

  16. Kurzawski M, Dziedziejko V, Urasińska E et al (2012) Nuclear factor erythroid 2- like 2 (Nrf2) expression in end-stage liver disease. Environ Toxicol Pharmacol 34(1):87–95

    Article  CAS  Google Scholar 

  17. Linkermann A, Green DR (2014) Necroptosis. N Engl J Med 370(5):455–465

    Article  CAS  Google Scholar 

  18. McConnachie LA, Mohar I, Hudson FN et al (2007) Glutamate cysteine ligase modifier subunit deficiency and gender as determinants of acetaminophen-induced hepatotoxicity in mice. Toxicol Sci 99(2):628–636

    Article  CAS  Google Scholar 

  19. Pasparakis M, Vandenabeele P (2015) Necroptosis and its role in inflammation. Nature 517(7534):311–320

    Article  CAS  Google Scholar 

  20. Teskey G, Abrahem R et al (2018) Glutathione as a marker for human disease. Adv Clin Chem 87:141–159

    Article  CAS  Google Scholar 

  21. Li ZX, Xu C et al (2017) Schisandrasphenanthera extract (WuzhiTablet) protects against chronic-binge and acute alcohol-induced liver injury by regulating the NRF2-ARE pathway in mice. Acta Pharm Sin B 7:583–592

    Article  Google Scholar 

  22. Ning H et al (2009) MicroRNA regulation of neuron-like differentiation of adipose tissue-derived stem cells. Differ Res Biol Divers 78(5):253–259

    Article  CAS  Google Scholar 

  23. Gonsky Y I, Korda M M, Klishch I N (1992) Antioxidative effect of dimethyl sulfoxide in acute impairment of liver tissue with tetrachloromethane. Voprosy medizinskoi khimii 38(2):43–44.

Download references

Author information

Authors and Affiliations

  1. Ufa Research Institute of Occupational Health and Human Ecology, Ufa, Russian Federation

    Y. V. Valova, M. M. Ziatdinova, G. F. Mukhammadieva, D. O. Karimov, A. S. Fazlyeva, N. Yu. Khusnutdinova, T. G. Yakupova & E. F. Repina

  2. The Faculty of Biology, Genetics and Fundamental Medicine Department, Bashkir State University, Ufa, Russian Federation

    Y. V. Valova

Authors
  1. Y. V. Valova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. M. Ziatdinova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. F. Mukhammadieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. O. Karimov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. S. Fazlyeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. Yu. Khusnutdinova
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T. G. Yakupova
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. E. F. Repina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. V. Valova.

Ethics declarations

Conflict of interest

Y.V. Valova, G.F. Mukhammadieva, T.G. Yakupova, D.O. Karimov, M.M. Ziatdinova, A.S. Fazlyeva, N.Yu. Khusnutdinova, E.F. Repina, I.I. Zaidullin declare that they have no conflict of interests.

Ethical approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Ufa Research Institute of Occupational Medicine and Human Ecology (Date 30.01.2019/No.1-01).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Valova, Y.V., Ziatdinova, M.M., Mukhammadieva, G.F. et al. Gene expression regulating the cell cycle and gene of antioxidant protection system in toxic hepatitis. Toxicol. Environ. Health Sci. 14, 13–18 (2022). https://doi.org/10.1007/s13530-021-00114-5

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13530-021-00114-5

Keywords

Search

Navigation