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Effect of Food Colorants and Additives on the Hematological and Histological Characteristics of Albino Rats

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Abstract

Objective

Food colorants (synthetic, inorganic and natural) represent one of the major categories of food additives. Synthetic colorants were banned due to their adverse effects on animals and human. However, synthetic dyes are still in use because they are cheap, and stable.

Methods

In the present in vivo study conducted on female albino rats (Rattus norvegicus), the effect of sunset yellow (SY) and sodium benzoate (NaB) combinations on the hematological and histological profile was assessed. Different combinations of SY plus NaB were dissolved in water and administered daily to experimental rat groups for 12 weeks. Group 1 (control) received only water, group 2 received 5 mg SY plus 10 mg NaB, group 3 received 5 mg SY plus 100 mg NaB, group 4 received 50 mg SY plus 100 mg NaB, group 5 received 50 mg SY plus 10 mg NaB, group 6 received 200 mg SY plus 750 mg NaB, and group 7 received 20 mg SY plus 75 mg NaB. Histopathological examinations were performed on liver and kidney of rats at the end of the experiment.

Results

The results revealed a decrease in RBCs count, hematocrit, WBCs, MCV and Hb levels upon the administration of SY plus NaB. The results also showed no increase in MCH, MCHC and platelet count. Liver and kidney tissues showed some lesions due to the administration of the tested compounds in comparison to the control animals.

Conclusion

The chemical stress caused by the SY and NaB combinations caused some degenerative changes in the liver and kidneys of rats. It could be concluded that SY and NaB combinations causes some damage in liver and kidney tissues of experimental animals. Therefore, using SY and NaB combinations should be limited.

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References

  1. Public Law 85-929, Food Additives Amendment of 1958. USA, pp. 1784–1789, https://www.govinfo.gov/content/pkg/STATUTE-72/pdf/STATUTE-72-Pg1784.pdf (1958).

    Google Scholar 

  2. FAO/WHO. Evaluation of certain food additives and contaminants, thirty-seventh sixth report of the Joint FAO/WHO Expert Committee on Food Additives, WHO Technical Report Series, No. 806, Geneva (1991).

    Google Scholar 

  3. Federal Register. 62 FR 18937, April 17. In Department of Agribusiness and Applied Economics, North Dakota State University, Fargo, ND 58105-5636 (1997).

    Google Scholar 

  4. Amchova, P., Kotolova, H. & Ruda-Kucerova, J. Health safety issues of synthetic food colorants. Reg. Toxicol. Pharmacol. 73, 914–922 (2015).

    Article  CAS  Google Scholar 

  5. FAO/WHO. Class names and the international numbering system for food additives, http://www.codexalimentarius.org (2014).

    Google Scholar 

  6. Helal, E. G. E., Zaahkouk, S. A. M. & Mekkawy, H. A. Effect of some food colourants (synthetic and natural products) of young albino rats. Egypt J. Hospital Med. 1, 103–113 (2000).

    CAS  Google Scholar 

  7. Soltan, S. S. A. & Shehata, M. M. E. M. The effects of using color foods of children on immunity properties and liver, kidney on rats. Food Nutr. Sci. 3, 897–904 (2012).

    CAS  Google Scholar 

  8. Sharma, U. K., Kumar, R., Gupta, A., Ganguly, R. & Pandey, A. K. Renoprotective effect of cinnamaldehyde in food color induced toxicity. 3 Biotech. 8, 212 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  9. Sharma, U. K. et al. Ameliorating efficacy of eugenol against metanil yellow induced toxicity in albino Wistar rats. Food Chem. Toxicol. 126, 34–40 (2019).

    Article  CAS  PubMed  Google Scholar 

  10. Garfield, S. in Mauve: How One Man Invented a Color that Changed the World (W.W. Norton & Company, New York, 2002).

    Google Scholar 

  11. Chaitanya, L. G. Food coloring: the natural way. Res. J. Chem. Sci. 4, 87–96 (2014).

    Google Scholar 

  12. Ali, M. Y., Hassan, G. M., Hassan, A. M. S., Mohamed, Z. A. & Ramadan, M. F. In vivo genotoxicity assessment of sunset yellow and sodium benzoate in female rats. Drug Chem. Toxicol. in press, https://doi.org/10.1080.01480545.2018.1510416 (2019).

    Google Scholar 

  13. Kobylewski, S. & Jacobson, M. F. Toxicology of food dyes. Inter. J. Occupat. Environ. Health 18, 220–246 (2012).

    Article  CAS  Google Scholar 

  14. Radwan, S. A., El-Sayed, A. R., Al-Shinnawy, M. S. & Mohamed, O. N. A. Hematological and biochemical changes induced by amaranth impact on male albino rats. Egypt J. Hospital Med. 40, 335–349 (2010).

    Google Scholar 

  15. Kusic, H., Juretic, D., Koprivanac, N., Marin, V. & Božic, A. L. Photooxidation processes for an azo dye in aqueous media: Modeling of degradation kinetic and ecological parameters evaluation. J. Hazard. Mat. 185, 1558–1568 (2011).

    Article  CAS  Google Scholar 

  16. Committee on Food Chemicals Codex. Food Chemicals Codex. Fifth edition. National Academy Press, Washington DC, p. 463 (2003).

    Google Scholar 

  17. Wood, R., Foster, L., Damant, A. & Key, P. in Analytical Methods for Food Additives, 1–14 (CRC Press, Boca Raton, 2004).

    Book  Google Scholar 

  18. EFSA European Food Safety Authority. Scientific opinion on the re-evaluation of sunset yellow FCF (E110) as a food additive. EFSA panel on food additives and nutrient sources added to food (ANS). EFSA Journal, Parma, pp. 1330 (2009).

    Google Scholar 

  19. Kus, E. & Eroglu, H. E. Genotoxic and cytotoxic effects of sunset yellow and brilliant blue, colorant food additives, on human blood lymphocytes. Pak. J. Pharm. Sci. 28, 227–230 (2015).

    CAS  PubMed  Google Scholar 

  20. Bhattacharjee, M. Evaluation of mitodepressive effect of sunset yellow using Allium sativum assay. Inter. J. Sci. Environ. Technol. 3, 1120–1130 (2014).

    Google Scholar 

  21. Dwivedi, K. & Kumar, G. Genetic damage induced by a food coloring dye (sunset yellow) on meristematic cells of Brassica campestris L. J. Environ. Public Health. Article ID 319727, http://dx.doi.org/10.1155/2015/319 727 (2015).

    Google Scholar 

  22. Paumgartten, F. J. R. et al. Evaluation of the developmental toxicity of annatto in the rat. Food Chem. Toxicol. 40, 1595–1601 (2002).

    Article  CAS  PubMed  Google Scholar 

  23. Kitamura, Y. et al. A subchronic toxicity study of shea nut color in Wister rats. Food Chem. Toxicol. 41, 1537–1542.(2003).

    Article  CAS  PubMed  Google Scholar 

  24. Bautista, A. R. P. L., Moreira, E. L. T., Batista, M. S., Miranda, M. S. & Gomes, I. C. S. Subacute toxicity assessment of annatto in rat. Food Chem. Toxicol. 42, 625–629 (2004).

    Article  CAS  PubMed  Google Scholar 

  25. Attia, Z. I., Basyuni, M. A., Hegazi, M. A. & Okba, S. G. Effect of benzene sulfonic acid, the degraded derivative of the sunset yellow beverages, coloring dyes on the physiology and growth rate of young rats. Egypt. J. Zool. 45, 373–387 (2005).

    Google Scholar 

  26. Bordagaray, A., Garcia-Arrona, R., Vidal, M. & Ostra, M. Determination of food colorants in a wide variety of food matrices by microemulsion electrokinetic capillary chromatography. Considerations on the found concentrations and regulated consumption limits. Food Chem. 262, 129–133 (2018).

    Article  CAS  PubMed  Google Scholar 

  27. Mathiyalagan, S., Mandal, B. K. & Ling, Y. C. Determination of synthetic and natural colorants in selected green colored foodstuffs through reverse phase-high performance liquid chromatography. Food Chem. 278, 381–387 (2019).

    Article  CAS  PubMed  Google Scholar 

  28. Sasaki, Y. F. et al. The Comet assay with 8 mouse organs: results with 39 currently used food additives. Mut. Res. Gene. Toxicol. Environ. Mutagen. 519, 103–119 (2002).

    Article  CAS  Google Scholar 

  29. Shimada, C., Kiyoshi, K., Sasaki, Yu. F., Sato, I. & Tsuda, S. Differential colon DNA damage induced by azo food additives between rats and mice. J. Toxicol. Sci. 35, 547–554 (2010).

    Article  CAS  PubMed  Google Scholar 

  30. Sayed, H. M., Fouad, D., Ataya, F. S., Hassanf, N. H. A. & Fahmy, M. A. The modifying effect of selenium and vitamins A, C, and E on the genotoxicity induced by sunset yellow in male mice. Mut. Res. 744, 145–153 (2012).

    Article  CAS  Google Scholar 

  31. Seetaramaiah, K., Anton Smith, A., Murali, R. & Manavalan, R. Preservatives in food products-review. Inter. J. Pharma. Biol. Arch. 2, 583–599 (2011).

    Google Scholar 

  32. Gülçin, I. Antioxidant activity of food constituents: an overview. Arch. Toxicol. 86, 345–391 (2012).

    Article  CAS  PubMed  Google Scholar 

  33. Gülçin, I., Elmastas, M. & Aboul-Eneinc, H. Y. Antioxidant activity of clove oil-A powerful antioxidant source. Arab. J. Chem. 5, 489–499 (2012).

    Article  CAS  Google Scholar 

  34. Gülçin, I. & Beydemir, S. Phenolic compounds as antioxidants: carbonic anhydrase isoenzymes inhibitors. Mini Rev. Med. Chem. 13, 408–430 (2013).

    PubMed  Google Scholar 

  35. Abdulmumeen, H. A., Risikat, A. N. & Sururah, A. R. Food: Its preservatives, additives and applications. IJCBS 1, 36–47 (2012).

    Google Scholar 

  36. Bursal, E., Köksal, E., Gülçin, I., Bilsel, G. & Gören, A. C. Antioxidant activity and polyphenol content of cherry stem (Cerasus avium L.) determined by LC-MS/MS. Food Res. Inter. 51, 66–74 (2013).

    Article  CAS  Google Scholar 

  37. Köse, L. P. et al. LC-MS/MS analysis, antioxidant and anticholinergic properties of galanga (Alpinia officinarum Hance) rhizomes. Ind. Crops Prod. 74, 712–721 (2015).

    Article  CAS  Google Scholar 

  38. Tohma, H. et al. Antioxidant activity and phenolic compounds of ginger (Zingiber officinale Rosc.) determined by HPLC-MS/MS. Food Meas. 11, 556–566 (2017).

    Article  Google Scholar 

  39. Lennerz, B. S. et al. Effects of sodium benzoate, a widely used food preservative, on glucose homeostasis and metabolic profiles in humans. Mol. Gene. Metabol. 114, 73–79 (2015).

    Article  CAS  Google Scholar 

  40. Zengin, N., Yüzbasioḡlu, D., Ünal, F., Yilmaz, S. & Aksoy, H. The evaluation of the genotoxicity of two food preservatives: Sodium benzoate and potassium benzoate. Food Chem. Toxicol. 49, 763–769 (2011).

    Article  CAS  PubMed  Google Scholar 

  41. Prival, J. M., Simmon, F. V. & Mortelmans, E. K. Bacterial mutagenicity testing of 49 food ingredients gives very few positive results. Mut. Res. 260, 321–329 (1991).

    Article  CAS  Google Scholar 

  42. Ishidate, M. et al. Primary mutagenicity screening of food additives currently used in Japan. Food Chem. Toxicol. 22, 623–636 (1984).

    Article  CAS  PubMed  Google Scholar 

  43. Xing, W. & Zhang, Z. A comparison of SCE test in human lymphocytes and Vicia faba: a hopeful technique using plants to detect mutagens and carcinogens. Mut. Res. 241, 109–113 (1990).

    Article  CAS  Google Scholar 

  44. Türkoglu, S. Genotoxicity of five food preservatives tested on root tips of Allium cepa L. Mut. Res. 626, 4–14.(1990).

    Article  CAS  Google Scholar 

  45. Mpountoukas, P., Vantarakis, A., Sivridis, E. & Lialiaris, T. Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives. Food Chem. Toxicol. 46, 2390–2393 (2008).

    Article  CAS  PubMed  Google Scholar 

  46. Demir, E., Kocaoglu, S. & Kaya, B. Assessment of genotoxic effects of benzyl derivatives by the Comet assay. Food Chem. Toxicol. 48, 1239–1242 (2010).

    Article  CAS  PubMed  Google Scholar 

  47. Uçar, A., Yilmaz, S., Yilmaz, S. & Kiliç, M. S. A research on the genotoxicity of stevia in human lymphocytes. Drug Chem. Toxicol. 41, 221–224 (2017).

    Article  CAS  PubMed  Google Scholar 

  48. Vijayan, V. & Mazumder, A. In vitro inhibition of food borne mutagens induced mutagenicity by cinnamon (Cinnamomum cassia) bark extract. Drug Chem. Toxicol. 41, 385–393 (2018).

    Article  CAS  PubMed  Google Scholar 

  49. Kaslow, J. E. Red blood cell count, hemoglobin, hematocrit, MCV, MCH, MCHC, platelet count, and random distribution of width, http://www.drkaslow.com/html/blood_cell_counts.html (2015).

    Google Scholar 

  50. Knezevich, A. L. & Hogan, G. K. A long-term oral toxicity/carcinogenicity study of green No. 3 in rats. The 29 h meeting of the joint FAO/WHO Expert on food additives committee, University Press Cambridge, Cambridge, UK (1981).

    Google Scholar 

  51. Sharma, A., Goyal, R. P., Chakravarty, G. & Sharma, S. Haemotoxic effect of chocolate brown, a commonly used blend of permitted food colour on Swiss albino mice. Asian J. Exper. Sci. 19, 93–103 (2005).

    CAS  Google Scholar 

  52. Sharma, A., Goyal, R. P., Chakravarty, G. & Sharma, S. Orange-red a permitted food colors induced hematological changes in albino mice, Mus musculus. Bull. Pure App. Sci. 24A, 99–103 (2005).

    CAS  Google Scholar 

  53. Sharma, S. D. & Iqbal, M. Lithium induced toxicity in rats: A hematological, biochemical and histopathological study. Biol. Pharm. Bull. 28, 834–837 (2005).

    Article  CAS  PubMed  Google Scholar 

  54. Sharma, S., Goyal, R. P., Chakravarty, G. & Sharma, A. Hematological and serological changes in the blood of albino mice, Mus Musculus. Ind. J. Environ. Sci. 10, 145–148 (2006).

    Google Scholar 

  55. Chakravarty, G., Goyal, R. P., Sharma, S. & Sharma, A. Hematological changes induced by a common non-permitted food colour, malachite green (MG) in Swiss albino mice. Ind. J. Environ. Sci. 9, 113–117 (2005).

    Google Scholar 

  56. Ashour, A. A. & Abdel-Aziz, I. Role of fast green on the blood of rats and the therapeutic action of vitamins C or E. Inter. J. Integ. Biol. 6, 6–11 (2009).

    CAS  Google Scholar 

  57. Khanna, S. K., Singh, G. B. & Singh, S. B. Non-permitted colours in food and their toxicity. J. Food Sci. Technol. 10, 33–36 (1973).

    CAS  Google Scholar 

  58. Lee, G. R. et al. Wintrobe’s Clinical Hematology (10th ed.), Baltimore, Maryland. Williams and Wilkins, Waverly Company (1998).

    Google Scholar 

  59. Helal, E. G. E. et al. Biochemical studies on the effect of ponceau 4r and/or vitamin E treatment on young male albino rats. Egypt. J. Hosp. Med. 31, 233–243 (2008).

    CAS  Google Scholar 

  60. Robert, A. & Budinsky, J. R. in Principles of Toxicology, Environmental and Industrial Applications. Hematotoxicity: Chemically Induced Toxicity of the Blood (eds Williams, P. L., James, R. C. & Roberts, S. M.) 87–110 (Wiley-Interscience Publication, New York, 2000).

  61. Hashem, M. M., Atta, A. H., Arbid, M. S., Somaia, A. N. M. & Gihan, F. A. Immunological studies on amaranth, sunset yellow and curcumin as food coloring agents in albino rats. Food Chem. Toxicol. 48, 1581–1586 (2010).

    Article  CAS  PubMed  Google Scholar 

  62. Himri, I. et al. A 90-day oral toxicity of tartrazine, a synthetic food dye, in rats. Inter. J. Pharm. Pharm. Sci. 3, 159–169 (2011).

    CAS  Google Scholar 

  63. Aboel-Zahab, H. et al. Physiological effects of some synthetic food colouring additives on rats. Bollettino Chimico Farmaceutico 136, 615–627 (1997).

    CAS  PubMed  Google Scholar 

  64. Ching, F. P., Akpan, J. O., Ekpo, M. D. & Ekanem, J. A. Acute in-vivo histological effect of food colorants on some rat tissues. Global J. Pure Appl. Sci. 11, 241–247 (2005).

    CAS  Google Scholar 

  65. Sarkar, R. & Ghosh, A. R. Metanil yellow an azo dye induced histopathological and ultrastructural changes in albino rat (Rattus norvegicus). The Bioscan 7, 427–432 (2012).

    CAS  Google Scholar 

  66. Khidr, B. M., Makhlouf, M. M. & Ahmed, S. M. M. Histological and ultrastructural study on the effect of sodium benzoate on the liver of adult male albino rats. Assiut Univ. J. Zoo. 41, 11–39 (2012).

    CAS  Google Scholar 

  67. El-Shamy, K. I., Khadr, M. E., Morsy, F. A. & Hassanin, M. M. Toxic effect of some food additives on the vital activities of albino rats: green colour (tartrazine and brilliant blue). Egypt. J. Zoo. 32, 417–440 (1999).

    Google Scholar 

  68. Elwi, M. A., Saleh, A. S. & Kamel, A. I. in Textbook of Pathology. Necrosis, 31–46 (El-Naser Modern Bookshop, Cairo, Egypt, 1973).

    Google Scholar 

  69. El-Banhawy, M. A. & Ganzuri, M. A. Pathological effects of insecticides on acid phosphatase containing particles in mammalian liver cells. Proc. Egypt Soc. Environ. Sci. 55–70 (1980).

    Google Scholar 

  70. Popper, H. & Kent, G. Fibrosis in chronic liver disease. Clinical Gastroenterol. 4, 315–332 (1975).

    CAS  Google Scholar 

  71. Svegliati Baroni, G. et al. Fibrogenic effect of oxidative stress on rat hepatic stellate cells. Hepatol. 27, 720–726 (1998).

    Article  CAS  Google Scholar 

  72. Bakar, E. & Aktaç, T. Effects of sodium benzoate and citric acid on serum, liver and kidney tissue total sialic acid levels: an ultrastructural study. J. Appl. Biol. Sci. 8, 9–15 (2014).

    CAS  Google Scholar 

  73. Itokawa, Y., Abe, T., Tabei, R. & Tanaka, S. Renal and skeletal lesions in experimental cadmium poisoning. Arch. Environ. Health 28, 149–154 (1974).

    Article  CAS  PubMed  Google Scholar 

  74. Gerundo, N., Alderman, D. J., Clifton-Hadely, R. S. & Feist, S. W. Pathological effects of repeated doses of malachite green. A preliminary study. J. Fish Diseases 14, 20–29 (1991).

    Article  Google Scholar 

  75. Hook, J. B. in Toxic Response of the Kidney. Casarett and Doull’s Toxicology. The Basic Science of Poisons (2nd ed.) 237 (Macmillan Publishing Co., New York, 1975.

    Google Scholar 

  76. Jones, D. B. in Kidneys. Anderson’s Pathology (ed Kissane, J. M.) 730 (The C.V. Mosby Company, St. Louis, MO, 1985).

  77. Cameron, G. R. in Pathology of the Cell (Edinburgh & London, Oliver and Boyd, 1952).

    Google Scholar 

  78. FAO/WHO. Safety evaluation of certain food additives and contaminants, seventy-third meeting of the Joint FAO/WHO Expert Committee on Food Additives, WHO Technical Report Series, No. 966, Geneva (2011).

    Google Scholar 

  79. European Food Safety Authority. Scientific opinion on the re-evaluation of sunset yellow as a food additive on request from the European Commission: Question No EFSA-Q-2008-224. European Food Safety Authority. The EFSA Journal; No. 1330. 10.2903/j.efsa.2009.1330 (2009).

    Google Scholar 

  80. Drabkin, D. L. & Austin, J. M. Spectrophotometric studies, spectrophotometric constants for common hemoglobin derivatives in human, dog and rabbit blood. J. Biol. Chem. 98, 719–733 (1932).

    CAS  Google Scholar 

  81. Dacie, J. V. & Lewis, S. M. in Practical Hematology, 37–113 (Churchill Living Stone, UK, 1993).

    Google Scholar 

  82. Becton-Dickinson. in Unopette WBC/Platelet Determination for Manual Method (Rutherford, N. J., Becton, Dickinson, 1996).

    Google Scholar 

  83. Clinical Laboratory Standards Institute. Procedure for determining packed cell volume by the microhematocrit method; approved standard (3rd). CLSI document H7-A3 (ISBN 1-56238-413-9). CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania, 19087-1898, USA (2000).

    Google Scholar 

  84. Bancroft, J. D. & Stevens, A. in Theory and Practice of Histological Techniqu (4th ed.) 41–60 (Churchill living stone, Melbourne, New York, USA, 1982).

    Google Scholar 

  85. SPSS. Statistical Package for Social Science (SPSS) User’s Guide: Statistics. Version 12, Chicago, U. S. A SPSS Inc (2003).

    Google Scholar 

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Ali, M.Y., Hassan, A.M.S., Mohamed, Z.A. et al. Effect of Food Colorants and Additives on the Hematological and Histological Characteristics of Albino Rats. Toxicol. Environ. Health Sci. 11, 155–167 (2019). https://doi.org/10.1007/s13530-019-0400-x

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