Histopathological Effects of Bisphenol A on Soft Tissues of Corbicula fluminea Mull
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Bisphenol A (BPA), a commonly occurring industrial chemical that is present in polycarbonate plastics and epoxy resins is mechanistically shown to affect various bodily functions of organisms. However, very limited studies have been done on the histological effects of BPA on bivalves. In this study, the toxicity of BPA was analyzed through its histological effects on the gills, digestive glands and adductor muscles of Corbicula fluminea, a freshwater bivalve.
Forty C. fluminea were exposed to set-ups with 1 µg/L, 2 µg/L and 3 µg/L of BPA for twenty-one days. Afterwhich, histolopathological analysis were done in the adductor muscles, digestive glands and gills of the clam. Histological alterations such as vacuolations, necrosis, lamellar deformation, hyperplasia, loss of epithelium, necrosis, tubular alteration, neoplasia, hemocyte infiltration, hypertrophy and pyknosis were observed and percent histological aberrations were determined per organ.
Results showed that there was a significant difference in the histological alterations observed between the tissues of exposed and unexposed clams. Moreover, varying concentrations of BPA rendered differential degree of histological damage on the soft tissues of the clam. The digestive gland was the most affected tissues followed by the gill then the adductor muscles.
BPA were found to be toxic to C. fluminea as evidenced by histology. Moreover, the differential histological responses of the tissues of C. fluminea in different concentrations of BPA proves that they are good indicators of environmental stressors such as BPA.
KeywordsCorbicula fluminea Histology Adductor muscles Digestive gland Gills Bisphenol-A
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Authors are thankful to the Department of Biology, University of the Philippines Manila for providing required laboratory facilities for this work.
- 11.Mantecca, P., Vailati, G. & Bacchetta, R. Histological changes and Micronucleus induction in the Zebra mussel Dreissena polymorpha afterparaquat exposure. Histol. Histopathol. 21, 829–840 (2006).Google Scholar
- 13.Britton, J. C. & Morton, B. in A dissection guide, field and laboratory manual for the introduced bivalve Corbicula fluminea. Malacologia Rev. (Niwot, Colorado, U.S.A. 1982).Google Scholar
- 14.McMahon, R. F. in Ecology and classification of North American freshwater invertebrates (eds Thorp, J. H., Covich, A. P.) 331–430 2nd Edn. (Academic Press, San Diego, 2001).Google Scholar
- 15.Ruppert, E. E., Fox, R. S. & Barnes R. B. in Invertebrate Zoology, A functional evolutionary Approach 7th Edn. (Brooks Cole Thomson, Belmont California, 2004).Google Scholar
- 18.Colombo, J. C., Bilos, C., Campanaho, M., Presa, M. J. R. & Catoggio, J. A. Bioaccumulation of polychlorinated-biphenys and chlorinated pesticides by the Asiatic Clam Corbicula fluminea—its use as sentinelorganism in the Rio-De-La-Plata Estuary, Argentina. Environ. Sci. Technol. 29, 914–927 (1995).CrossRefGoogle Scholar
- 19.Labrot, F., Narbonne, J. F., Ville, P., Saint Denis, M. & Ribera, D. Acute toxicity, toxicokinetics, and tissue target of lead and uranium in the clam Corbicula fluminea and the worm Eisenia fetida: comparison with the fish Bradydanio rerio. Arch. Environ. Contam. Toxicol. 36, 167–178 (1999).CrossRefGoogle Scholar
- 23.Baudrimont, M., Lemaire-Gony, S, Ribeyre, F., Metivaud, J. & Boudou, A. Seasonal variations of metallothionine concentrations in the Asiatic clam (Corbicula fluminea). Comp. Biochem. Physiol. C 118, 361–367 (1997).Google Scholar
- 32.Arriola, F. J. & Villaluz, D. K. Snail fishing and duck raising in Laguna de Bay, Luzon. Phil. J. Scie. 69, 173–187 (1939).Google Scholar
- 36.Kanapala, V. & Arasada, S. P. Histopathological effect of paraquat (gramoxene) on the digestive gland of fresh-water snail Lymnaea luteola (Lamarck: 1799) (mollusca: gastropoda). Int. J. Scien. Res. Environ. Sci. 1, 224–230 (2013).Google Scholar
- 43.Kumar, S., Pandey, R. K. & Das, V. K. Dimethoate alters respiratory rate and gill histopathology in freshwater mussel Lamellidens marginalis (Lamarck). J. Appl. Biosci. 38, 154–158 (2012).Google Scholar
- 44.Abdel-Nabi, I. M., El-Shenawy, N. S., Taha, I. A. & Moawad, T. I. Oxidative stress biomarkers and bioconcentration of Reldan and Roundup by the edible clam, Ruditapes decussates. Curr. Zool. 53, 910–920 (2007).Google Scholar
- 47.Fuller, J. K. in Surgical technology: principles and practice 6th Edn. (Elsevier Saunders, Missouri, 2013).Google Scholar
- 52.Rodriguez-Ariza, A. et al. Uptake and clearance of PCB congeners in Chamaelea gallina: response of oxidative stress biomarkers. Compar. Biochem. Physiol. C 134, 57–67 (2003).Google Scholar