Abstract
Lactic acid bacteria (LAB) provide numerous beneficial effects on the host body, especially on the intestine. Two LAB strains isolated from Kimchi, Leuconostoc mesenteroides and Lactobacillus sakei, were studied for its anti-inflammatory activity in acidinduced acute colitis in mice. To induce acute colitis in model mice (C57BL/6), 3% of dextran sulfate sodium treatment was treated for 7 days. Assessment of necropsy and histopathology analysis showed that oral supplementation of both L. mesenteroides and L. sakei ameliorated the symptoms of acute colitis. Moreover, the mixture of L. meseneroides and L. sakei showed synergistic effect on colitis. The results suggest that the formulation of L. mesenteroides and L. sakei mixture could be used as an oral supplementation to decrease the inflammatory harmful environment associated with colitis.
Similar content being viewed by others
References
Masood, M. I. et al. Beneficial effects of lactic acid bacteria on human beings. Crit. Rev. Microbiol. 37, 91–98 (2011).
Wells, J. M. Immunomodulatory mechanisms of Lactobacilli. Microb. Cell Fact. 10, doi:10.1186/1475-2859-10-S1-S17 (2011).
Turroni, F. et al. Molecular dialogue between the human gut microbiota and the host: a Lactobacillus and Bifidobacterium perspective. Cell. Mol. Life Sci. 71, 183–203 (2014).
Gabriela Perdigón, R. F. & Raúl Raya. Lactic acid bacteria and their effect on the immune system. Curr. Issues Intest. Microbiol. 2, 27–42 (2001).
Hörmannsperger, G. & Haller, D. Molecular crosstalk of probiotic bacteria with the intestinal immune system: Clinical relevance in the context of inflammatory bowel disease. Int. J. Med. Microbiol. 300, 63–73 (2010).
Fuller, R. Probiotics in man and animals. J. Appl. Bacteriol. 66, 365–378 (1989).
Lee, J. et al. Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice. BMC Microbiol. 16, doi:10.1186/ s12866-016-0686-7 (2016).
Maslowski, K. M. et al. Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43. Nature 461, 1282–1286 (2009).
Lee, J. S. et al. Classification of isolates originating from Kimchi using carbon-source utilization patterns. J. Microbiol. Biotechnol. 7, 68–74 (1997).
Lee, J.-S. et al. Weissella koreensis sp. nov., isolated from kimchi. Int. J. Syst. Evol. Microbiol. 52, 1257–1261 (2002).
Bae, J. W. et al. Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. Appl. Environ. Microbiol. 71, 8825–8835 (2005).
Jung, J. Y. et al. Metagenomic analysis of kimchi, a traditional Korean fermented food. Appl. Environ. Microbiol. 77, 2264–2274 (2011).
Kekkonen, R. A. et al. Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production. World J. Gastroenterol. 14, 1192–1203 (2008).
Kim, J. Y. et al. Atopic dermatitis-mitigating effects of new Lactobacillus strain, Lactobacillus sakei probio 65 isolated from Kimchi. J. Appl. Microbiol. 115, 517–526 (2013).
Sayer, B. et al. Dextran sodium sulphate-induced colitis perturbs muscarinic cholinergic control of colonic epithelial ion transport. Br. J. Pharmacol. 135, 1794–1800 (2002).
Paradis, V. & Bedossa, P. Definition and natural history of metabolic steatosis: histology and cellular aspects. Diabetes Metab. 6, 638–642 (2008).
Carvalho, R. D. O. et al. Use of wild type or recombinant lactic acid bacteria as an alternative treatment for gastrointestinal inflammatory diseases: a focus on inflammatory bowel diseases and mucositis. Front Microbiol. 8, doi:10.3389/fmicb.2017.00800 (2017).
Ren, C. et al. Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation. Sci. Rep. 6, doi:10.1038/srep34561 (2016).
Mattar, A. et al. Probiotics up-regulate MUC-2 mucin gene expression in a Caco-2-cell culture mogel. Pediatr. Surg. Int. 18, 586–590 (2002).
Ng, S. C. et al. Mechanisms of action of probiotics: recent advances. Inflamm. Bowel. Dis. 15, 300–310 (2009).
Moal, V. L.-L., Sarrazin-Davila, L. E. & Servin, A. L. An experimental study and a randomized, double-blind, placebo-controlled clinical trial to evaluate the antisecretory activity of Lactobacillus acidophilus strain LB against nanrotavirus diarrhea. Pediatrics 120, e795–803 (2007).
Kang, M.-G. et al. Anti-inflammatory effects of sucrose-derived oligosaccharides produced by a constitutive mutant L. mesenteroides B-512FMCM dextransucrase in high fat-diet mice. Biochem. Biophys. Res. Commun. 477, 350–355 (2016).
Lim, S.-M. et al. Lactobacillus sakei OK67 ameliorates high-fat diet-induced blood glucose intolerance and obesity in mice by inhigiting gut microbiota lipopolysaccharide production and inducing colon tight junction protein expression. Nutr. Res. 36, 337–348 (2016).
Kim, J.-Y. Atopic dermatitis-mitigating effects of new Lactobacillus strain, Lactobacillus sackei probio 65 isolated from Kimchi. J. Appl. Microbiol. 115, 517–526 (2013).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Lee, S.Y., Sekhon, S.S., Kim, H.C. et al. Anti-inflammatory effect of lactic acid bacteria isolated from kimchi on acid-induced acute colitis in model mice. Toxicol. Environ. Health Sci. 9, 279–283 (2017). https://doi.org/10.1007/s13530-017-0332-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13530-017-0332-2